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Title: Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications

A near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. We achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb 3+ ) in the UCNP shell, which bridged the energy transfer from the dye to the UCNP core. As a result, we synergized the two most practical upconversion booster effectors (dye-sensitizing and core/shell enhancement) to amplify upconversion efficiency. We also demonstrated two biomedical applications using these UCNPs. By using dye-sensitized core/active shell UCNP embedded poly(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a biocompatible and deep tissue penetrable 800 nm wavelength. Furthermore, UCNPs were water-solubilized with Pluronic F127 with high upconversion efficiency and can be imaged in a mouse model.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [2] ;  [2] ;  [4] ;  [5] ;  [6] ;  [7] ;  [8] ;  [3] ;  [3] ;  [2]
  1. Univ. of Massachusetts Medical School, Worcester, MA (United States). Dept. of Biochemistry and Molecular Pharmacology; Dalian Univ. of Technology (People's Republic of China)
  2. Univ. of Massachusetts Medical School, Worcester, MA (United States). Dept. of Biochemistry and Molecular Pharmacology
  3. Univ. of Massachusetts Medical School, Worcester, MA (United States). Neurobiology Dept.
  4. Zhejiang Univ., Hangzhou (China).. Dept. of Materials Science and Engineering
  5. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  6. Univ. of Massachusetts, Amherst, MA (United States). Chemical Engineering Dept.
  7. Dalian Univ. of Technology (People's Republic of China). State Key Lab. of Fine Chemicals
  8. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). The Molecular Foundry
Publication Date:
Grant/Contract Number:
AC02-05CH11231; R01MH103133; RGY-0090/2014
Type:
Accepted Manuscript
Journal Name:
ACS Nano
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1936-0851
Publisher:
American Chemical Society (ACS)
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; bioimaging; core/active shell structure; dye-sensitizing; near-infrared; optogenetics; upconversion nanoparticles
OSTI Identifier:
1379046