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Title: An Enrichment of CRISPR and Other Defense-Related Features in Marine Sponge-Associated Microbial Metagenomes

Abstract

Many marine sponges are populated by dense and taxonomically diverse microbial consortia. We employed a metagenomics approach to unravel the differences in the functional gene repertoire among three Mediterranean sponge species, Petrosia ficiformis, Sarcotragus foetidus, Aplysina aerophoba and seawater. Different signatures were observed between sponge and seawater metagenomes with regard to microbial community composition, GC content, and estimated bacterial genome size. Our analysis showed further a pronounced repertoire for defense systems in sponge metagenomes. Specifically, clustered regularly interspaced short palindromic repeats, restriction modification, DNA phosphorothioation and phage growth limitation systems were enriched in sponge metagenomes. These data suggest that defense is an important functional trait for an existence within sponges that requires mechanisms to defend against foreign DNA from microorganisms and viruses. Furthermore, this study contributes to an understanding of the evolutionary arms race between viruses/phages and bacterial genomes and it sheds light on the bacterial defenses that have evolved in the context of the sponge holobiont.

Authors:
 [1];  [1];  [1];  [2];  [3];  [4];  [4];  [5]
  1. GEOMAR Helmholtz Centre for Ocean Research, Kiel (Germany); Univ. of Wurzburg, Wurzburg (Germany)
  2. GEOMAR Helmholtz Centre for Ocean Research, Kiel (Germany)
  3. Univ. of New South Wales, Sydney, NSW (Australia)
  4. Univ. of Wurzburg, Wurzburg (Germany)
  5. GEOMAR Helmholtz Centre for Ocean Research, Kiel (Germany); Christian-Albrechts-Univ. zu Kiel, Kiel (Germany)
Publication Date:
Research Org.:
GEOMAR Helmholtz Centre for Ocean Research, Kiel (Germany). Marine Microbiology
Sponsoring Org.:
USDOE
OSTI Identifier:
1378945
Grant/Contract Number:  
311932
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; metagenomes; defense; CRISPR; restriction modification; sponge microbiome; seawater

Citation Formats

Horn, Hannes, Slaby, Beate M., Jahn, Martin T., Bayer, Kristina, Moitinho-Silva, Lucas, Forster, Frank, Abdelmohsen, Usama R., and Hentschel, Ute. An Enrichment of CRISPR and Other Defense-Related Features in Marine Sponge-Associated Microbial Metagenomes. United States: N. p., 2016. Web. doi:10.3389/fmicb.2016.01751.
Horn, Hannes, Slaby, Beate M., Jahn, Martin T., Bayer, Kristina, Moitinho-Silva, Lucas, Forster, Frank, Abdelmohsen, Usama R., & Hentschel, Ute. An Enrichment of CRISPR and Other Defense-Related Features in Marine Sponge-Associated Microbial Metagenomes. United States. doi:10.3389/fmicb.2016.01751.
Horn, Hannes, Slaby, Beate M., Jahn, Martin T., Bayer, Kristina, Moitinho-Silva, Lucas, Forster, Frank, Abdelmohsen, Usama R., and Hentschel, Ute. Tue . "An Enrichment of CRISPR and Other Defense-Related Features in Marine Sponge-Associated Microbial Metagenomes". United States. doi:10.3389/fmicb.2016.01751. https://www.osti.gov/servlets/purl/1378945.
@article{osti_1378945,
title = {An Enrichment of CRISPR and Other Defense-Related Features in Marine Sponge-Associated Microbial Metagenomes},
author = {Horn, Hannes and Slaby, Beate M. and Jahn, Martin T. and Bayer, Kristina and Moitinho-Silva, Lucas and Forster, Frank and Abdelmohsen, Usama R. and Hentschel, Ute},
abstractNote = {Many marine sponges are populated by dense and taxonomically diverse microbial consortia. We employed a metagenomics approach to unravel the differences in the functional gene repertoire among three Mediterranean sponge species, Petrosia ficiformis, Sarcotragus foetidus, Aplysina aerophoba and seawater. Different signatures were observed between sponge and seawater metagenomes with regard to microbial community composition, GC content, and estimated bacterial genome size. Our analysis showed further a pronounced repertoire for defense systems in sponge metagenomes. Specifically, clustered regularly interspaced short palindromic repeats, restriction modification, DNA phosphorothioation and phage growth limitation systems were enriched in sponge metagenomes. These data suggest that defense is an important functional trait for an existence within sponges that requires mechanisms to defend against foreign DNA from microorganisms and viruses. Furthermore, this study contributes to an understanding of the evolutionary arms race between viruses/phages and bacterial genomes and it sheds light on the bacterial defenses that have evolved in the context of the sponge holobiont.},
doi = {10.3389/fmicb.2016.01751},
journal = {Frontiers in Microbiology},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {11}
}

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Cited by: 12 works
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    Works referencing / citing this record:

    Phylogeny and genomics of SAUL, an enigmatic bacterial lineage frequently associated with marine sponges: Phylogeny and genomics of SAUL
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    Phylogeny and genomics of SAUL, an enigmatic bacterial lineage frequently associated with marine sponges: Phylogeny and genomics of SAUL
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