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Title: Neurospora crassa transcriptomics reveals oxidative stress and plasma membrane homeostasis biology genes as key targets in response to chitosan

Chitosan is a natural polymer with antimicrobial activity. Chitosan causes plasma membrane permeabilization and induction of intracellular reactive oxygen species (ROS) in Neurospora crassa. In this paper, we have determined the transcriptional profile of N. crassa to chitosan and identified the main gene targets involved in the cellular response to this compound. Global network analyses showed membrane, transport and oxidoreductase activity as key nodes affected by chitosan. Activation of oxidative metabolism indicates the importance of ROS and cell energy together with plasma membrane homeostasis in N. crassa response to chitosan. Deletion strain analysis of chitosan susceptibility pointed NCU03639 encoding a class 3 lipase, involved in plasma membrane repair by lipid replacement, and NCU04537 a MFS monosaccharide transporter related to assimilation of simple sugars, as main gene targets of chitosan. NCU10521, a glutathione S-transferase-4 involved in the generation of reducing power for scavenging intracellular ROS is also a determinant chitosan gene target. Ca 2+ increased tolerance to chitosan in N. crassa. Growth of NCU10610 (fig 1 domain) and SYT1 (a synaptotagmin) deletion strains was significantly increased by Ca 2+ in the presence of chitosan. Both genes play a determinant role in N. crassa membrane homeostasis. Finally, our results are of paramountmore » importance for developing chitosan as an antifungal.« less
Authors:
ORCiD logo [1] ;  [2] ;  [3] ;  [2] ;  [2] ;  [1]
  1. Univ. of Alicante (Spain). Lab. of Plant Pathology, Multidisciplinary Inst. for Environmental Studies (MIES) Ramon Margalef, Dept. of Marine Sciences and Applied Biology
  2. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
  3. Univ. of Alicante (Spain). Statistics and Operation Research Dept.
Publication Date:
Grant/Contract Number:
AC02-05CH11231; GM060468; 2011-29297/AGR
Type:
Accepted Manuscript
Journal Name:
Molecular BioSystems
Additional Journal Information:
Journal Volume: 12; Journal Issue: 2; Journal ID: ISSN 1742-206X
Publisher:
Royal Society of Chemistry
Research Org:
Univ. of California, Berkeley, CA (United States); Univ. of Alicante (Spain)
Sponsoring Org:
USDOE; National Inst. of Health (NIH) (United States); Ministry of Economy and Competitiveness (Spain)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; drug targets; time-series analysis; ROS; membrane remodeling; calcium
OSTI Identifier:
1378742