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Title: Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae

Abstract

Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogenXanthomonas oryzae pv.oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunityin planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higherXoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed inUbi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.

Authors:
 [1];  [2];  [2];  [1]
  1. Univ. of California, Davis, CA (United States). Department of Plant Pathology and the Genome Center; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Feedstocks Division, Joint BioEnergy Institute and Biological Systems and Engineering Division
  2. Univ. of California, Davis, CA (United States). Department of Plant Pathology and the Genome Center
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1378728
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
PeerJ
Additional Journal Information:
Journal Volume: 4; Journal Issue: 10; Journal ID: ISSN 2167-8359
Publisher:
PeerJ Inc.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Nuclear localization; Bacterial blight disease; Xanthomonas oryzae pv. oryzae; XA21-mediated immunity

Citation Formats

Wei, Tong, Chen, Tsung-Chi, Ho, Yuen Ting, and Ronald, Pamela C. Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae. United States: N. p., 2016. Web. doi:10.7717/peerj.2507.
Wei, Tong, Chen, Tsung-Chi, Ho, Yuen Ting, & Ronald, Pamela C. Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae. United States. doi:10.7717/peerj.2507.
Wei, Tong, Chen, Tsung-Chi, Ho, Yuen Ting, and Ronald, Pamela C. Wed . "Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae". United States. doi:10.7717/peerj.2507. https://www.osti.gov/servlets/purl/1378728.
@article{osti_1378728,
title = {Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae},
author = {Wei, Tong and Chen, Tsung-Chi and Ho, Yuen Ting and Ronald, Pamela C.},
abstractNote = {Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogenXanthomonas oryzae pv.oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunityin planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higherXoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed inUbi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.},
doi = {10.7717/peerj.2507},
journal = {PeerJ},
number = 10,
volume = 4,
place = {United States},
year = {2016},
month = {10}
}

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Works referenced in this record:

Rice XB15, a Protein Phosphatase 2C, Negatively Regulates Cell Death and XA21-Mediated Innate Immunity
journal, September 2008


A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21
journal, December 1995


Towards Establishment of a Rice Stress Response Interactome
journal, April 2011


Plant and Animal Sensors of Conserved Microbial Signatures
journal, November 2010


Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice
journal, February 2010


Rice NRR, a negative regulator of disease resistance, interacts with Arabidopsis NPR1 and rice NH1: NRR, a negative regulator of resistance
journal, August 2005


Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA
journal, August 1994


The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium
journal, July 2015

  • Pruitt, Rory N.; Schwessinger, Benjamin; Joe, Anna
  • Science Advances, Vol. 1, Issue 6
  • DOI: 10.1126/sciadv.1500245

Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21 -Mediated Disease Resistance
journal, December 2006


An ATPase promotes autophosphorylation of the pattern recognition receptor XA21 and inhibits XA21-mediated immunity
journal, April 2010

  • Chen, Xuewei; Chern, Mawsheng; Canlas, Patrick E.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 17
  • DOI: 10.1073/pnas.0912311107