Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
Abstract
In this paper, we describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. Finally, we used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).
- Authors:
-
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- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst.
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
- Humboldt Univ. of Berlin (Germany). Inst. of Biology
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
- Stanford Univ., CA (United States). School of Medicine. Dept. of Structural Biology
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst. Stanford Synchrotron Radiation Lightsource
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Laboratory Directed Research and Development (LDRD) Program; National Inst. of Health (NIH) (United States); Human Frontier Science Program (HFSP) (France)
- Contributing Org.:
- Humboldt Univ. of Berlin (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
- OSTI Identifier:
- 1378710
- Grant/Contract Number:
- AC02-05CH11231; AC02-76SF00515; P41GM103393; P41RR001209; GM51266; GM082545; GM055302; GM110501; GM095887; GM102520; RGP0005/2011; RGP0063/2013 310
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Methods
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 1; Journal ID: ISSN 1548-7091
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; microfluidics; molecular biophysics; nanocrystallography; proteins
Citation Formats
Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., and DeMirci, Hasan. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. United States: N. p., 2015.
Web. doi:10.1038/nmeth.3667.
Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., & DeMirci, Hasan. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. United States. doi:10.1038/nmeth.3667.
Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., and DeMirci, Hasan. Mon .
"Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II". United States. doi:10.1038/nmeth.3667. https://www.osti.gov/servlets/purl/1378710.
@article{osti_1378710,
title = {Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II},
author = {Sierra, Raymond G. and Gati, Cornelius and Laksmono, Hartawan and Dao, E. Han and Gul, Sheraz and Fuller, Franklin and Kern, Jan and Chatterjee, Ruchira and Ibrahim, Mohamed and Brewster, Aaron S. and Young, Iris D. and Michels-Clark, Tara and Aquila, Andrew and Liang, Mengning and Hunter, Mark S. and Koglin, Jason E. and Boutet, Sébastien and Junco, Elia A. and Hayes, Brandon and Bogan, Michael J. and Hampton, Christina Y. and Puglisi, Elisabetta V. and Sauter, Nicholas K. and Stan, Claudiu A. and Zouni, Athina and Yano, Junko and Yachandra, Vittal K. and Soltis, S. Michael and Puglisi, Joseph D. and DeMirci, Hasan},
abstractNote = {In this paper, we describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. Finally, we used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).},
doi = {10.1038/nmeth.3667},
journal = {Nature Methods},
number = 1,
volume = 13,
place = {United States},
year = {2015},
month = {11}
}
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