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Title: Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran

Abstract

A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). Finally, none of the samplesmore » were positive for Pestiviruses.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6]
+ Show Author Affiliations
  1. Univ. of Adelaide, SA (Australia). School of Animal and Veterinary Sciences
  2. Univ. of Adelaide, SA (Australia). School of Animal and Veterinary Sciences; Univ. of South Australia, Adelaide, SA (Australia). School of Pharmacy and Medical Sciences
  3. Univ. of Tehran, Tehran (Iran). Faculty of Veterinary Medicine
  4. Islamic Azad Univ., Shahrekord (Iran)
  5. Iran Dept. of Environment, Tehran (Iran)
  6. Katholieke Universiteit Leuven Rega Institute for Medical Research, Belgium
Publication Date:
Research Org.:
Univ. of Adelaide, SA (Australia). School of Animal and Veterinary Sciences
Sponsoring Org.:
Iran DoE
OSTI Identifier:
1378484
Grant/Contract Number:  
01-1384
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 12; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Hemmatzadeh, Farhid, Boardman, Wayne, Alinejad, Arezo, Hematzade, Azar, Moghadam, Majid Kharazian, and Andrei, Graciela. Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0168756.
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Hemmatzadeh, Farhid, Boardman, Wayne, Alinejad, Arezo, Hematzade, Azar, Moghadam, Majid Kharazian, & Andrei, Graciela. Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran. United States. doi:10.1371/journal.pone.0168756.
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Hemmatzadeh, Farhid, Boardman, Wayne, Alinejad, Arezo, Hematzade, Azar, Moghadam, Majid Kharazian, and Andrei, Graciela. Tue . "Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran". United States. doi:10.1371/journal.pone.0168756. https://www.osti.gov/servlets/purl/1378484.
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@article{osti_1378484,
title = {Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran},
author = {Hemmatzadeh, Farhid and Boardman, Wayne and Alinejad, Arezo and Hematzade, Azar and Moghadam, Majid Kharazian and Andrei, Graciela},
abstractNote = {A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). Finally, none of the samples were positive for Pestiviruses.},
doi = {10.1371/journal.pone.0168756},
journal = {PLoS ONE},
number = 12,
volume = 11,
place = {United States},
year = {2016},
month = {12}
}
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DOI:  10.1371/journal.pone.0168756
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