Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran

doi:10.1371/journal.pone.0168756

Title: Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran

Abstract

A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). Finally, none of the samplesmore » were positive for Pestiviruses.« less

Authors:

Hemmatzadeh, Farhid ^[1]; Boardman, Wayne ^[2]; Alinejad, Arezo ^[3]; Hematzade, Azar ^[4]; Moghadam, Majid Kharazian ^[5]; Andrei, Graciela ^[6]

Univ. of Adelaide, SA (Australia). School of Animal and Veterinary Sciences
Univ. of Adelaide, SA (Australia). School of Animal and Veterinary Sciences; Univ. of South Australia, Adelaide, SA (Australia). School of Pharmacy and Medical Sciences
Univ. of Tehran, Tehran (Iran). Faculty of Veterinary Medicine
Islamic Azad Univ., Shahrekord (Iran)
Iran Dept. of Environment, Tehran (Iran)
Katholieke Universiteit Leuven Rega Institute for Medical Research, Belgium

Publication Date:: 2016-12-20

Research Org.:: Univ. of Adelaide, SA (Australia). School of Animal and Veterinary Sciences

Sponsoring Org.:: Iran DoE

OSTI Identifier:: 1378484

Grant/Contract Number:: 01-1384

Resource Type:: Accepted Manuscript

Journal Name:: PLoS ONE

Additional Journal Information:: Journal Volume: 11; Journal Issue: 12; Journal ID: ISSN 1932-6203

Publisher:: Public Library of Science

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Hemmatzadeh, Farhid, Boardman, Wayne, Alinejad, Arezo, Hematzade, Azar, Moghadam, Majid Kharazian, and Andrei, Graciela. Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran.  United States: N. p., 2016. 
        Web.  doi:10.1371/journal.pone.0168756.

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                    Hemmatzadeh, Farhid, Boardman, Wayne, Alinejad, Arezo, Hematzade, Azar, Moghadam, Majid Kharazian, & Andrei, Graciela. Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran.  United States.  doi:10.1371/journal.pone.0168756.

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                    Hemmatzadeh, Farhid, Boardman, Wayne, Alinejad, Arezo, Hematzade, Azar, Moghadam, Majid Kharazian, and Andrei, Graciela. Tue .  
        "Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran".  United States.  doi:10.1371/journal.pone.0168756.  https://www.osti.gov/servlets/purl/1378484.

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@article{osti_1378484,

  title        = {Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran},

  author       = {Hemmatzadeh, Farhid and Boardman, Wayne and Alinejad, Arezo and Hematzade, Azar and Moghadam, Majid Kharazian and Andrei, Graciela},

  abstractNote = {A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). Finally, none of the samples were positive for Pestiviruses.},

  doi          = {10.1371/journal.pone.0168756},

  journal      = {PLoS ONE},

  number       = 12,

  volume       = 11,

  place        = {United States},

  year         = {2016},

  month        = {12}

}

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DOI: 10.1371/journal.pone.0168756

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Works referenced in this record:

Aspects of the persistence of foot-and-mouth disease virus in animals—the carrier problem
journal, August 2002

Alexandersen, Soren; Zhang, Zhidong; Donaldson, Alex I.
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Alexandersen, S.; Zhang, Z.; Donaldson, A. I.
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Foot and Mouth Disease: a look from the wild side
journal, October 2013

Weaver, Genevieve V.; Domenech, Joseph; Thiermann, Alex R.
Journal of Wildlife Diseases, Vol. 49, Issue 4
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Similar Records

Title: Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran

Abstract

Citation Formats

Aspects of the persistence of foot-and-mouth disease virus in animals—the carrier problem journal, August 2002

Emerging Infectious Diseases of Wildlife-- Threats to Biodiversity and Human Health journal, January 2000

The Pathogenesis and Diagnosis of Foot-and-Mouth Disease journal, July 2003

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journal, August 2002

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journal, July 2003

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journal, January 2003

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journal, August 2013

Foot and Mouth Disease: a look from the wild side
journal, October 2013