Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions
Abstract
Foot-and-mouth disease (FMD) is a highly contagious and economically important viral disease of cloven-hoofed animals, including domestic and wild host species. During recent FMD outbreaks in India, spontaneous abortions were reported amongst FMD-affected and asymptomatic cows. The current study was an opportunistic investigation of these naturally occurring bovine abortions to assess causality of abortion and vertical transmission of FMDV from infected cows to fetuses. For this purpose, fetal tissue samples of eight abortuses (heart, liver, kidney, spleen, palatine tonsil, umbilical cord, soft palate, tongue, lungs, and submandibular lymph node) were collected and screened by various detection methods, including viral genome detection, virus isolation, and immunomicroscopy. Amongst these cases, gross pathological changes were observed in 3 abortuses. Gross pathological findings included blood-tinged peritoneal and pleural effusions and myocarditis. Hearts of infected calves had mild to moderate degeneration and necrosis of the myocardium with moderate infiltration by mixed inflammatory cells. Localization of FMDV antigen was demonstrated in lungs and soft palate by immunomicroscopy. FMDV serotype O viral genome was recovered from 7 of 8 cases. Infectious FMDV serotype O was rescued by chemical transfection of the total RNA extracted from three soft palate samples and was sequenced to confirm 100% identity ofmore »
- Authors:
-
- ICAR- Directorate of Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand (India)
- Indian Veterinary Research Inst. (IVRI), Izatnagar, Bareilly, Uttar Pradesh (India). Centre for Animal Disease Research and Diagnosis (CADRAD)
- US Dept. of Agriculture (USDA), Greenport, NY (United States). Agricultural Research Service, Plum Island Animal Disease Center, Foreign Animal Disease Research Unit
- US Dept. of Agriculture (USDA), Greenport, NY (United States). Agricultural Research Service, Plum Island Animal Disease Center, Foreign Animal Disease Research Unit; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
- Foreign Animal Disease Research Unit, USDA-ARS, Plum Island Animal Disease Center, Greenport, New York (United States)
- Publication Date:
- Research Org.:
- US Dept. of Agriculture (USDA), Greenport, NY (United States). Agricultural Research Service
- Sponsoring Org.:
- USDOE; USDA
- OSTI Identifier:
- 1378471
- Grant/Contract Number:
- 1940-32000-057-00D; HSHQDC-12-X-0060
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 12; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Ranjan, Rajeev, Biswal, Jitendra K., Subramaniam, Saravanan, Singh, Karam Pal, Stenfeldt, Carolina, Rodriguez, Luis L., Pattnaik, Bramhadev, and Arzt, Jonathan. Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions. United States: N. p., 2016.
Web. doi:10.1371/journal.pone.0167163.
Ranjan, Rajeev, Biswal, Jitendra K., Subramaniam, Saravanan, Singh, Karam Pal, Stenfeldt, Carolina, Rodriguez, Luis L., Pattnaik, Bramhadev, & Arzt, Jonathan. Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions. United States. doi:10.1371/journal.pone.0167163.
Ranjan, Rajeev, Biswal, Jitendra K., Subramaniam, Saravanan, Singh, Karam Pal, Stenfeldt, Carolina, Rodriguez, Luis L., Pattnaik, Bramhadev, and Arzt, Jonathan. Thu .
"Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions". United States. doi:10.1371/journal.pone.0167163. https://www.osti.gov/servlets/purl/1378471.
@article{osti_1378471,
title = {Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions},
author = {Ranjan, Rajeev and Biswal, Jitendra K. and Subramaniam, Saravanan and Singh, Karam Pal and Stenfeldt, Carolina and Rodriguez, Luis L. and Pattnaik, Bramhadev and Arzt, Jonathan},
abstractNote = {Foot-and-mouth disease (FMD) is a highly contagious and economically important viral disease of cloven-hoofed animals, including domestic and wild host species. During recent FMD outbreaks in India, spontaneous abortions were reported amongst FMD-affected and asymptomatic cows. The current study was an opportunistic investigation of these naturally occurring bovine abortions to assess causality of abortion and vertical transmission of FMDV from infected cows to fetuses. For this purpose, fetal tissue samples of eight abortuses (heart, liver, kidney, spleen, palatine tonsil, umbilical cord, soft palate, tongue, lungs, and submandibular lymph node) were collected and screened by various detection methods, including viral genome detection, virus isolation, and immunomicroscopy. Amongst these cases, gross pathological changes were observed in 3 abortuses. Gross pathological findings included blood-tinged peritoneal and pleural effusions and myocarditis. Hearts of infected calves had mild to moderate degeneration and necrosis of the myocardium with moderate infiltration by mixed inflammatory cells. Localization of FMDV antigen was demonstrated in lungs and soft palate by immunomicroscopy. FMDV serotype O viral genome was recovered from 7 of 8 cases. Infectious FMDV serotype O was rescued by chemical transfection of the total RNA extracted from three soft palate samples and was sequenced to confirm 100% identity of the VP1 (capsid) coding region with isolates collected from infected cattle during the acute phase of infection. Based upon these findings, it may be concluded that FMDV-associated abortion occurred among the infected pregnant cows included within this study and FMDV was subsequently transmitted vertically to fetuses. This is the first documentation of FMDV-associated abortions in cattle.},
doi = {10.1371/journal.pone.0167163},
journal = {PLoS ONE},
number = 12,
volume = 11,
place = {United States},
year = {2016},
month = {12}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 7 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Foot-and-Mouth Disease
journal, April 2004
- Grubman, M. J.; Baxt, B.
- Clinical Microbiology Reviews, Vol. 17, Issue 2
The Early Pathogenesis of Foot-and-Mouth Disease in Cattle After Aerosol Inoculation: Identification of the Nasopharynx as the Primary Site of Infection
journal, June 2010
- Arzt, J.; Pacheco, J. M.; Rodriguez, L. L.
- Veterinary Pathology, Vol. 47, Issue 6
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle
journal, November 2015
- Stenfeldt, Carolina; Eschbaumer, Michael; Pacheco, Juan M.
- PLOS ONE, Vol. 10, Issue 11
Early events in the pathogenesis of foot-and-mouth disease in cattle after controlled aerosol exposure
journal, January 2010
- Pacheco, Juan M.; Arzt, Jonathan; Rodriguez, Luis L.
- The Veterinary Journal, Vol. 183, Issue 1
Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A
journal, July 2014
- Stenfeldt, Carolina; Pacheco, Juan M.; Borca, Manuel V.
- Acta Veterinaria Scandinavica, Vol. 56, Issue 1
Persistent Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Cattle; Tissue-Specific Distribution and Local Cytokine Expression
journal, May 2015
- Pacheco, Juan M.; Smoliga, George R.; O’Donnell, Vivian
- PLOS ONE, Vol. 10, Issue 5
Molecular epidemiology of foot-and-mouth disease virus
journal, January 2003
- Knowles, N. J.; Samuel, A. R.
- Virus Research, Vol. 91, Issue 1
Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues
journal, July 1999
- Prato Murphy, Mariana L.; Forsyth, Morag A.; Belsham, Graham J.
- Virus Research, Vol. 62, Issue 1
Foot-and-Mouth Disease Virus Persists in the Light Zone of Germinal Centres
journal, October 2008
- Juleff, Nicholas; Windsor, Miriam; Reid, Elizabeth
- PLoS ONE, Vol. 3, Issue 10
Relationship Between Clinical Signs and Transmission of an Infectious Disease and the Implications for Control
journal, May 2011
- Charleston, B.; Bankowski, B. M.; Gubbins, S.
- Science, Vol. 332, Issue 6030
The Pathogenesis of Foot-and-Mouth Disease II: Viral Pathways in Swine, Small Ruminants, and Wildlife; Myotropism, Chronic Syndromes, and Molecular Virus-Host Interactions: Foot-and-Mouth Disease Pathogenesis: Atypical Syndromes
journal, June 2011
- Arzt, J.; Baxt, B.; Grubman, M. J.
- Transboundary and Emerging Diseases, Vol. 58, Issue 4
The Pathogenesis of Foot-and-Mouth Disease I: Viral Pathways in Cattle: Foot-and-Mouth Disease Pathogenesis in Cattle
journal, March 2011
- Arzt, J.; Juleff, N.; Zhang, Z.
- Transboundary and Emerging Diseases, Vol. 58, Issue 4
Studies of Quantitative Parameters of Virus Excretion and Transmission in Pigs and Cattle Experimentally Infected with Foot-and-Mouth Disease Virus
journal, November 2003
- Alexandersen, S.; Quan, M.; Murphy, C.
- Journal of Comparative Pathology, Vol. 129, Issue 4
The Pathogenesis and Diagnosis of Foot-and-Mouth Disease
journal, July 2003
- Alexandersen, S.; Zhang, Z.; Donaldson, A. I.
- Journal of Comparative Pathology, Vol. 129, Issue 1
The Localization of Persistent Foot and Mouth Disease Virus in the Epithelial Cells of the Soft Palate and Pharynx
journal, February 2001
- Zhang, Z. D.; Kitching, R. P.
- Journal of Comparative Pathology, Vol. 124, Issue 2-3
The pathogenesis of natural and simulated natural foot-and-mouth disease infection in cattle
journal, October 1981
- Burrows, R.; Mann, J. A.; Garland, A. J. M.
- Journal of Comparative Pathology, Vol. 91, Issue 4
Studies on the carrier state of cattle exposed to foot-and-mouth disease virus
journal, March 1966
- Burrows, R.
- Journal of Hygiene, Vol. 64, Issue 1
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
journal, October 2013
- Tamura, Koichiro; Stecher, Glen; Peterson, Daniel
- Molecular Biology and Evolution, Vol. 30, Issue 12
The Foot-and-Mouth Disease Carrier State Divergence in Cattle
journal, May 2016
- Stenfeldt, Carolina; Eschbaumer, Michael; Rekant, Steven I.
- Journal of Virology, Vol. 90, Issue 14
Foot and mouth disease virus transmission during the incubation period of the disease in piglets, lambs, calves, and dairy cows
journal, February 2009
- Orsel, K.; Bouma, A.; Dekker, A.
- Preventive Veterinary Medicine, Vol. 88, Issue 2