The Need for Integrated Approaches in Metabolic Engineering
Abstract
Highlights include state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. A combination of different approaches over multiple time and size scales must be considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the “system” that is being manipulated: transcriptome, translatome, proteome, or reactome. Here, by bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.
- Authors:
-
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Chemical & Biomolecular Engineering, Dept. of Bioengineering
- Univ. of California, San Diego, CA (United States). Dept. of Bioengineering
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Chemical & Biomolecular Engineering, Dept. of Bioengineering; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory, E-Scholarship Repository, Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1378363
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Cold Spring Harbor Perspectives in Biology
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 11; Journal ID: ISSN 1943-0264
- Publisher:
- Cold Spring Harbor
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Lechner, Anna, Brunk, Elizabeth, and Keasling, Jay D. The Need for Integrated Approaches in Metabolic Engineering. United States: N. p., 2016.
Web. doi:10.1101/cshperspect.a023903.
Lechner, Anna, Brunk, Elizabeth, & Keasling, Jay D. The Need for Integrated Approaches in Metabolic Engineering. United States. https://doi.org/10.1101/cshperspect.a023903
Lechner, Anna, Brunk, Elizabeth, and Keasling, Jay D. Mon .
"The Need for Integrated Approaches in Metabolic Engineering". United States. https://doi.org/10.1101/cshperspect.a023903. https://www.osti.gov/servlets/purl/1378363.
@article{osti_1378363,
title = {The Need for Integrated Approaches in Metabolic Engineering},
author = {Lechner, Anna and Brunk, Elizabeth and Keasling, Jay D.},
abstractNote = {Highlights include state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. A combination of different approaches over multiple time and size scales must be considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the “system” that is being manipulated: transcriptome, translatome, proteome, or reactome. Here, by bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.},
doi = {10.1101/cshperspect.a023903},
journal = {Cold Spring Harbor Perspectives in Biology},
number = 11,
volume = 8,
place = {United States},
year = {Mon Aug 15 00:00:00 EDT 2016},
month = {Mon Aug 15 00:00:00 EDT 2016}
}
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journal, July 2018
- Crook, Nathan; Ferreiro, Aura; Gasparrini, Andrew J.
- Cell Host & Microbe
Gas fermentation: cellular engineering possibilities and scale up
journal, April 2017
- Heijstra, Björn D.; Leang, Ching; Juminaga, Alex
- Microbial Cell Factories, Vol. 16, Issue 1