Dear microtubule, I see you

Nogales, Eva

doi:10.1091/mbc.E16-06-0372

Title: Dear microtubule, I see you

Abstract

This essay summarizes my personal journey toward the atomic visualization of microtubules and a mechanistic understanding of how these amazing polymers work. During this journey, I have been witness and partaker in the blooming of a technique I love—cryo-electron microscopy.

Authors:

Nogales, Eva ^[1]

Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst. Molecular and Cell Biology Dept.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging

Publication Date:: Tue Nov 01 00:00:00 EDT 2016

Research Org.:: Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE; National Inst. of Health (NIH) (United States)

OSTI Identifier:: 1377552

Grant/Contract Number:: AC02-05CH11231; GM051487

Resource Type:: Accepted Manuscript

Journal Name:: Molecular Biology of the Cell

Additional Journal Information:: Journal Volume: 27; Journal Issue: 21; Journal ID: ISSN 1059-1524

Publisher:: American Society for Cell Biology

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Nogales, Eva. Dear microtubule, I see you.  United States: N. p., 2016. 
Web.  doi:10.1091/mbc.E16-06-0372.

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                    Nogales, Eva. Dear microtubule, I see you.  United States.  https://doi.org/10.1091/mbc.E16-06-0372

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                    Nogales, Eva. Tue .  
"Dear microtubule, I see you".  United States.  https://doi.org/10.1091/mbc.E16-06-0372.  https://www.osti.gov/servlets/purl/1377552.

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@article{osti_1377552,

  title        = {Dear microtubule, I see you},

  author       = {Nogales, Eva},

  abstractNote = {This essay summarizes my personal journey toward the atomic visualization of microtubules and a mechanistic understanding of how these amazing polymers work. During this journey, I have been witness and partaker in the blooming of a technique I love—cryo-electron microscopy.},

  doi          = {10.1091/mbc.E16-06-0372},

  journal      = {Molecular Biology of the Cell},

  number       = 21,

  volume       = 27,

  place        = {United States},

  year         = {Tue Nov 01 00:00:00 EDT 2016},

  month        = {Tue Nov 01 00:00:00 EDT 2016}

}

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Journal Article:

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Accepted Manuscript (DOE)

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https://doi.org/10.1091/mbc.E16-06-0372

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Figures / Tables:

FIGURE 1: Microtubule and tubulin structures then and now. (A) A 20 Å cryo-EM density map (gray mesh) shown for three protofilaments, with the alpha trace of the docked electron crystallographic structure of the protofilament shown in green, except for one tubulin dimer highlighted in blue (modified from Nogales etmore »

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Works referenced in this record:

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Figures / Tables found in this record:

FIGURE 1 (p. 3)

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Similar Records

Title: Dear microtubule, I see you

Abstract

Citation Formats

Figures / Tables:

Microtubule Structure at 8 Å Resolution journal, October 2002

Refined structure of αβ-tubulin at 3.5 Å resolution 1 1Edited by I. A. Wilson journal, November 2001

Crystal structure of the kinesin motor domain reveals a structural similarity to myosin journal, April 1996

EBs Recognize a Nucleotide-Dependent Structural Cap at Growing Microtubule Ends journal, April 2012

Multimodal microtubule binding by the Ndc80 kinetochore complex journal, October 2012

Tubulin and FtsZ form a distinct family of GTPases journal, June 1998

Three-dimensional structure of functional motor proteins on microtubules journal, October 1996

Electron microscopy of frozen biological suspensions journal, January 1983

An atomic-level mechanism for activation of the kinesin molecular motors journal, February 2010

Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy journal, June 1990

Dynamic instability of microtubule growth journal, November 1984

Structural Basis for Microtubule Binding and Release by Dynein journal, September 2012

Characterization of microtubule protofilament numbers journal, April 1990

Structure of the αβ tubulin dimer by electron crystallography journal, January 1998

Structural Basis of Interprotofilament Interaction and Lateral Deformation of Microtubules journal, August 2010

High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis journal, May 2014

Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules. journal, September 1996

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins journal, August 2015

High-Resolution Model of the Microtubule journal, January 1999

Microtubule Structure at 8 Å Resolution
journal, October 2002

Refined structure of αβ-tubulin at 3.5 Å resolution 1 1Edited by I. A. Wilson
journal, November 2001

Crystal structure of the kinesin motor domain reveals a structural similarity to myosin
journal, April 1996

EBs Recognize a Nucleotide-Dependent Structural Cap at Growing Microtubule Ends
journal, April 2012

Multimodal microtubule binding by the Ndc80 kinetochore complex
journal, October 2012

Tubulin and FtsZ form a distinct family of GTPases
journal, June 1998

Three-dimensional structure of functional motor proteins on microtubules
journal, October 1996

Electron microscopy of frozen biological suspensions
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An atomic-level mechanism for activation of the kinesin molecular motors
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Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy
journal, June 1990

Dynamic instability of microtubule growth
journal, November 1984

Structural Basis for Microtubule Binding and Release by Dynein
journal, September 2012

Characterization of microtubule protofilament numbers
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Structure of the αβ tubulin dimer by electron crystallography
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Structural Basis of Interprotofilament Interaction and Lateral Deformation of Microtubules
journal, August 2010

High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis
journal, May 2014

Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules.
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Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins
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