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Title: OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice

Abstract

Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Several dozen CESA mutants have been reported since cellulose synthase (CESA) gene was first identified, but almost all mutants exhibit the defective phenotypes in plant growth and development. Here, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P-CR conserved site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%–41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co-IP detection, together with implementations of a proteasome inhibitor (MG132) and two distinct cellulose inhibitors (Calcofluor, CGA), shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low-DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3-fold and ethanol productivity by 34%–42%. Our study has for the first time reported a direct modification for the low-DP cellulose production that has broadmore » applications in biomass industries.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]; ORCiD logo [2];  [1]
  1. Huazhong Agricultural Univ. Wuhan (China)
  2. Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1376598
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 15; Journal Issue: 9; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; biomass saccharification; cellulose; CESA; lodging resistance; rice

Citation Formats

Li, Fengcheng, Xie, Guosheng, Huang, Jiangfeng, Zhang, Ran, Li, Yu, Zhang, Miaomiao, Wang, Yanting, Li, Ao, Li, Xukai, Xia, Tao, Qu, Chengcheng, Hu, Fan, Ragauskas, Arthur J., and Peng, Liangcai. OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice. United States: N. p., 2017. Web. doi:10.1111/pbi.12700.
Li, Fengcheng, Xie, Guosheng, Huang, Jiangfeng, Zhang, Ran, Li, Yu, Zhang, Miaomiao, Wang, Yanting, Li, Ao, Li, Xukai, Xia, Tao, Qu, Chengcheng, Hu, Fan, Ragauskas, Arthur J., & Peng, Liangcai. OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice. United States. doi:10.1111/pbi.12700.
Li, Fengcheng, Xie, Guosheng, Huang, Jiangfeng, Zhang, Ran, Li, Yu, Zhang, Miaomiao, Wang, Yanting, Li, Ao, Li, Xukai, Xia, Tao, Qu, Chengcheng, Hu, Fan, Ragauskas, Arthur J., and Peng, Liangcai. Wed . "OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice". United States. doi:10.1111/pbi.12700. https://www.osti.gov/servlets/purl/1376598.
@article{osti_1376598,
title = {OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice},
author = {Li, Fengcheng and Xie, Guosheng and Huang, Jiangfeng and Zhang, Ran and Li, Yu and Zhang, Miaomiao and Wang, Yanting and Li, Ao and Li, Xukai and Xia, Tao and Qu, Chengcheng and Hu, Fan and Ragauskas, Arthur J. and Peng, Liangcai},
abstractNote = {Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Several dozen CESA mutants have been reported since cellulose synthase (CESA) gene was first identified, but almost all mutants exhibit the defective phenotypes in plant growth and development. Here, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P-CR conserved site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%–41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co-IP detection, together with implementations of a proteasome inhibitor (MG132) and two distinct cellulose inhibitors (Calcofluor, CGA), shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low-DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3-fold and ethanol productivity by 34%–42%. Our study has for the first time reported a direct modification for the low-DP cellulose production that has broad applications in biomass industries.},
doi = {10.1111/pbi.12700},
journal = {Plant Biotechnology Journal},
number = 9,
volume = 15,
place = {United States},
year = {2017},
month = {3}
}

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Works referenced in this record:

Calcofluor white ST Alters the in vivo assembly of cellulose microfibrils
journal, November 1980


The new frontier of genome engineering with CRISPR-Cas9
journal, November 2014


Plant cell wall reconstruction toward improved lignocellulosic production and processability
journal, February 2010


Cell-wall carbohydrates and their modification as a resource for biofuels
journal, May 2008


Sitosterol-beta -glucoside as Primer for Cellulose Synthesis in Plants
journal, January 2002


Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1A903V and CESA3T942I of cellulose synthase
journal, February 2012

  • Harris, D. M.; Corbin, K.; Wang, T.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 11
  • DOI: 10.1073/pnas.1200352109

Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem
journal, March 2007

  • Islam, M. Sirajul; Peng, Shaobing; Visperas, Romeo M.
  • Field Crops Research, Vol. 101, Issue 2
  • DOI: 10.1016/j.fcr.2006.12.002

Determination of ethanol in distilled liquors using sequential injection analysis with spectrophotometric detection
journal, December 2003


Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

  • Chen, Fang; Dixon, Richard A.
  • Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
  • DOI: 10.1038/nbt1316

Disruption of Secondary Wall Cellulose Biosynthesis Alters Cadmium Translocation and Tolerance in Rice Plants
journal, May 2013

  • Song, Xue-Qin; Liu, Li-Feng; Jiang, Yi-Jun
  • Molecular Plant, Vol. 6, Issue 3
  • DOI: 10.1093/mp/sst025

Control of tillering in rice
journal, April 2003


A Gibberellin-Mediated DELLA-NAC Signaling Cascade Regulates Cellulose Synthesis in Rice
journal, May 2015

  • Huang, Debao; Wang, Shaogan; Zhang, Baocai
  • The Plant Cell, Vol. 27, Issue 6
  • DOI: 10.1105/tpc.15.00015

How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


Interactions among three distinct CesA proteins essential for cellulose synthesis
journal, January 2003

  • Taylor, N. G.; Howells, R. M.; Huttly, A. K.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 3
  • DOI: 10.1073/pnas.0337628100

Pausing of Golgi Bodies on Microtubules Regulates Secretion of Cellulose Synthase Complexes in Arabidopsis
journal, April 2009

  • Crowell, E. F.; Bischoff, V.; Desprez, T.
  • The Plant Cell, Vol. 21, Issue 4, p. 1141-1154
  • DOI: 10.1105/tpc.108.065334

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

The Structure of the Catalytic Domain of a Plant Cellulose Synthase and Its Assembly into Dimers
journal, July 2014


An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer
journal, October 1959


Stem and root characteristics associated with lodging resistance in four winter wheat cultivars
journal, October 1994


Hemicelluloses negatively affect lignocellulose crystallinity for high biomass digestibility under NaOH and H2SO4 pretreatments in Miscanthus
journal, January 2012

  • Xu, Ning; Zhang, Wei; Ren, Shuangfeng
  • Biotechnology for Biofuels, Vol. 5, Issue 1
  • DOI: 10.1186/1754-6834-5-58

Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies
journal, September 2009


Tertiary model of a plant cellulose synthase
journal, April 2013

  • Sethaphong, L.; Haigler, C. H.; Kubicki, J. D.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 18
  • DOI: 10.1073/pnas.1301027110

The Ubiquitin 26s Proteasome Proteolytic Pathway
journal, June 2004


Cellulose Synthesis in Higher Plants
journal, November 2006


Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall
journal, August 2003

  • Tanaka, Katsuyuki; Murata, Kazumasa; Yamazaki, Muneo
  • Plant Physiology, Vol. 133, Issue 1
  • DOI: 10.1104/pp.103.022442

Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase.
journal, October 1996

  • Pear, J. R.; Kawagoe, Y.; Schreckengost, W. E.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 22
  • DOI: 10.1073/pnas.93.22.12637

Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014


Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

  • Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
  • Nature, Vol. 509, Issue 7500, p. 376-380
  • DOI: 10.1038/nature13084

Dimerization of cotton fiber cellulose synthase catalytic subunits occurs via oxidation of the zinc-binding domains
journal, August 2002

  • Kurek, I.; Kawagoe, Y.; Jacob-Wilk, D.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 17
  • DOI: 10.1073/pnas.162077099

Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils
journal, August 2013


Expression profiling and integrative analysis of the CESA/CSL superfamily in rice
journal, January 2010


Plant cell wall engineering: applications in biofuel production and improved human health
journal, April 2014


XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan
journal, October 2012

  • Chiniquy, D.; Sharma, V.; Schultink, A.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 42
  • DOI: 10.1073/pnas.1202079109

DWARF3 Participates in an SCF Complex and Associates with DWARF14 to Suppress Rice Shoot Branching
journal, April 2014

  • Zhao, Jinfeng; Wang, Tao; Wang, Minxia
  • Plant and Cell Physiology, Vol. 55, Issue 6
  • DOI: 10.1093/pcp/pcu045

    Works referencing / citing this record:

    Combined mild chemical pretreatments for complete cadmium release and cellulosic ethanol co-production distinctive in wheat mutant straw
    journal, January 2019

    • Cheng, Liangliang; Wang, Lingqiang; Wei, Luyao
    • Green Chemistry, Vol. 21, Issue 13
    • DOI: 10.1039/c9gc00686a

    A novel FC17/CESA4 mutation causes increased biomass saccharification and lodging resistance by remodeling cell wall in rice
    journal, November 2018


    Combined mild chemical pretreatments for complete cadmium release and cellulosic ethanol co-production distinctive in wheat mutant straw
    journal, January 2019

    • Cheng, Liangliang; Wang, Lingqiang; Wei, Luyao
    • Green Chemistry, Vol. 21, Issue 13
    • DOI: 10.1039/c9gc00686a

    A novel FC17/CESA4 mutation causes increased biomass saccharification and lodging resistance by remodeling cell wall in rice
    journal, November 2018