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Title: Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina

Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammonium sulfate as carbon and nitrogen sources, respectively. No oxidation of As(III) to As(V) was observed under any conditions. Here, three copies of the ars operon comprising arsenic resistance genes were found on B. linens AE038-8 genome. In addition to the well known arsC, ACR3 and arsR, two copies of the arsO gene of unknown function were detected.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [5] ;  [2]
  1. PROIMI-CONICET- Univ. Nacional de Tucuman, Tucuman (Argentina)
  2. U.S. Geological Survey, Menlo Park, CA (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  5. Florida Intl Univ., Miami, FL (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
International Biodeterioration and Biodegradation
Additional Journal Information:
Journal Volume: 107; Journal Issue: C; Journal ID: ISSN 0964-8305
Publisher:
Elsevier
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; Arsenic-resistance; Brevibacterium linens; Groundwater
OSTI Identifier:
1376317
Alternate Identifier(s):
OSTI ID: 1359168

Maizel, Daniela, Blum, Jodi Switzer, Ferrero, Marcela A., Utturkar, Sagar M., Brown, Steven D., Rosen, Barry P., and Oremland, Ronald S.. Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina. United States: N. p., Web. doi:10.1016/j.ibiod.2015.11.022.
Maizel, Daniela, Blum, Jodi Switzer, Ferrero, Marcela A., Utturkar, Sagar M., Brown, Steven D., Rosen, Barry P., & Oremland, Ronald S.. Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina. United States. doi:10.1016/j.ibiod.2015.11.022.
Maizel, Daniela, Blum, Jodi Switzer, Ferrero, Marcela A., Utturkar, Sagar M., Brown, Steven D., Rosen, Barry P., and Oremland, Ronald S.. 2015. "Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina". United States. doi:10.1016/j.ibiod.2015.11.022. https://www.osti.gov/servlets/purl/1376317.
@article{osti_1376317,
title = {Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina},
author = {Maizel, Daniela and Blum, Jodi Switzer and Ferrero, Marcela A. and Utturkar, Sagar M. and Brown, Steven D. and Rosen, Barry P. and Oremland, Ronald S.},
abstractNote = {Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammonium sulfate as carbon and nitrogen sources, respectively. No oxidation of As(III) to As(V) was observed under any conditions. Here, three copies of the ars operon comprising arsenic resistance genes were found on B. linens AE038-8 genome. In addition to the well known arsC, ACR3 and arsR, two copies of the arsO gene of unknown function were detected.},
doi = {10.1016/j.ibiod.2015.11.022},
journal = {International Biodeterioration and Biodegradation},
number = C,
volume = 107,
place = {United States},
year = {2015},
month = {12}
}