Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina
Abstract
Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammonium sulfate as carbon and nitrogen sources, respectively. No oxidation of As(III) to As(V) was observed under any conditions. Here, three copies of the ars operon comprising arsenic resistance genes were found on B. linens AE038-8 genome. In addition to the well known arsC, ACR3 and arsR, two copies of the arsO gene of unknown function were detected.
- Authors:
-
- PROIMI-CONICET- Univ. Nacional de Tucuman, Tucuman (Argentina)
- U.S. Geological Survey, Menlo Park, CA (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Florida Intl Univ., Miami, FL (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1376317
- Alternate Identifier(s):
- OSTI ID: 1359168
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Biodeterioration and Biodegradation
- Additional Journal Information:
- Journal Volume: 107; Journal Issue: C; Journal ID: ISSN 0964-8305
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; Arsenic-resistance; Brevibacterium linens; Groundwater
Citation Formats
Maizel, Daniela, Blum, Jodi Switzer, Ferrero, Marcela A., Utturkar, Sagar M., Brown, Steven D., Rosen, Barry P., and Oremland, Ronald S. Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina. United States: N. p., 2015.
Web. doi:10.1016/j.ibiod.2015.11.022.
Maizel, Daniela, Blum, Jodi Switzer, Ferrero, Marcela A., Utturkar, Sagar M., Brown, Steven D., Rosen, Barry P., & Oremland, Ronald S. Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina. United States. https://doi.org/10.1016/j.ibiod.2015.11.022
Maizel, Daniela, Blum, Jodi Switzer, Ferrero, Marcela A., Utturkar, Sagar M., Brown, Steven D., Rosen, Barry P., and Oremland, Ronald S. Thu .
"Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina". United States. https://doi.org/10.1016/j.ibiod.2015.11.022. https://www.osti.gov/servlets/purl/1376317.
@article{osti_1376317,
title = {Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina},
author = {Maizel, Daniela and Blum, Jodi Switzer and Ferrero, Marcela A. and Utturkar, Sagar M. and Brown, Steven D. and Rosen, Barry P. and Oremland, Ronald S.},
abstractNote = {Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammonium sulfate as carbon and nitrogen sources, respectively. No oxidation of As(III) to As(V) was observed under any conditions. Here, three copies of the ars operon comprising arsenic resistance genes were found on B. linens AE038-8 genome. In addition to the well known arsC, ACR3 and arsR, two copies of the arsO gene of unknown function were detected.},
doi = {10.1016/j.ibiod.2015.11.022},
journal = {International Biodeterioration and Biodegradation},
number = C,
volume = 107,
place = {United States},
year = {Thu Dec 10 00:00:00 EST 2015},
month = {Thu Dec 10 00:00:00 EST 2015}
}
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