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Title: Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85

Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60–5.65, which is lower than has been reported previously. Wild type and acid tolerant strains digested corn stover with equal efficiency in batch culture at low pH. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.10 and 5.65 compared to pH 6.70, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored E. coli wild-type levels of acid tolerance, suggesting a possible role in acid homeostasis. Here, recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this bacterium.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [3]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Wisconsin, Madison, WI (United States)
  2. Biota Biosciences, Inc., Cambridge City, IN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Elanco Animal Health, Greenfield, IN (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Microbiology; Systems biology
OSTI Identifier:
1376309
Alternate Identifier(s):
OSTI ID: 1408644

Wu, Chia-wei, Spike, Thomas, Klingeman, Dawn M., Rodriguez, Miguel, Bremer, Virgil R., and Brown, Steven D.. Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85. United States: N. p., Web. doi:10.1038/s41598-017-02628-w.
Wu, Chia-wei, Spike, Thomas, Klingeman, Dawn M., Rodriguez, Miguel, Bremer, Virgil R., & Brown, Steven D.. Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85. United States. doi:10.1038/s41598-017-02628-w.
Wu, Chia-wei, Spike, Thomas, Klingeman, Dawn M., Rodriguez, Miguel, Bremer, Virgil R., and Brown, Steven D.. 2017. "Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85". United States. doi:10.1038/s41598-017-02628-w. https://www.osti.gov/servlets/purl/1376309.
@article{osti_1376309,
title = {Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85},
author = {Wu, Chia-wei and Spike, Thomas and Klingeman, Dawn M. and Rodriguez, Miguel and Bremer, Virgil R. and Brown, Steven D.},
abstractNote = {Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60–5.65, which is lower than has been reported previously. Wild type and acid tolerant strains digested corn stover with equal efficiency in batch culture at low pH. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.10 and 5.65 compared to pH 6.70, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored E. coli wild-type levels of acid tolerance, suggesting a possible role in acid homeostasis. Here, recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this bacterium.},
doi = {10.1038/s41598-017-02628-w},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {5}
}

Works referenced in this record:

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006
  • Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
  • Molecular Systems Biology, Vol. 2, Article No. 2006.0008
  • DOI: 10.1038/msb4100050

The Path Forward for Biofuels and Biomaterials
journal, January 2006
  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
journal, July 1995

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002