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Title: The role of Rnf in ion gradient formation in Desulfovibrio alaskensis

Abstract

Rnf is a membrane protein complex that has been shown to be important in energy conservation. Here, Desulfovibrio alaskensis G20 and Rnf mutants of G20 were grown with different electron donor and acceptor combinations to determine the importance of Rnf in energy conservation and the type of ion gradient generated. The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore ETH2120 had no effect, indicating a role for the proton gradient during growth. Mutants in rnfA and rnfD were more sensitive to the protonophore at 5 µM than the parental strain, suggesting the importance of Rnf in the generation of a proton gradient. The electrical potential (ΔΨ), ΔpH and proton motive force were lower in thernfAmutant than in the parental strain of D.alaskensis G20. In conclusion, these results provide evidence that the Rnf complex in D. alaskensis functions as a primary proton pump whose activity is important for growth.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Univ. of Oklahoma, Norman, OK (United States)
Publication Date:
Research Org.:
Office of Scientific and Technical Information, Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1375918
Resource Type:
Accepted Manuscript
Journal Name:
PeerJ
Additional Journal Information:
Journal Volume: 4; Journal ID: ISSN 2167-8359
Publisher:
PeerJ Inc.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Desulfovibrio; Rnf; proton motive force; ionophore; energetics

Citation Formats

Wang, Luyao, Bradstock, Peter, Li, Chuang, McInerney, Michael J., and Krumholz, Lee R. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. United States: N. p., 2016. Web. doi:10.7717/peerj.1919.
Wang, Luyao, Bradstock, Peter, Li, Chuang, McInerney, Michael J., & Krumholz, Lee R. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. United States. doi:10.7717/peerj.1919.
Wang, Luyao, Bradstock, Peter, Li, Chuang, McInerney, Michael J., and Krumholz, Lee R. Thu . "The role of Rnf in ion gradient formation in Desulfovibrio alaskensis". United States. doi:10.7717/peerj.1919. https://www.osti.gov/servlets/purl/1375918.
@article{osti_1375918,
title = {The role of Rnf in ion gradient formation in Desulfovibrio alaskensis},
author = {Wang, Luyao and Bradstock, Peter and Li, Chuang and McInerney, Michael J. and Krumholz, Lee R.},
abstractNote = {Rnf is a membrane protein complex that has been shown to be important in energy conservation. Here, Desulfovibrio alaskensis G20 and Rnf mutants of G20 were grown with different electron donor and acceptor combinations to determine the importance of Rnf in energy conservation and the type of ion gradient generated. The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore ETH2120 had no effect, indicating a role for the proton gradient during growth. Mutants in rnfA and rnfD were more sensitive to the protonophore at 5 µM than the parental strain, suggesting the importance of Rnf in the generation of a proton gradient. The electrical potential (ΔΨ), ΔpH and proton motive force were lower in thernfAmutant than in the parental strain of D.alaskensis G20. In conclusion, these results provide evidence that the Rnf complex in D. alaskensis functions as a primary proton pump whose activity is important for growth.},
doi = {10.7717/peerj.1919},
journal = {PeerJ},
number = ,
volume = 4,
place = {United States},
year = {2016},
month = {4}
}

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