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Title: Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Here, we present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a MetagenomeAssembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.
Authors:
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  1. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  2. Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
  3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); United States Dept. of Agriculture, Gainesville, FL (United States)
  4. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Merced, CA (United States)
  5. Broad Institute, Cambridge, MA (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  7. European Molecular Biology Lab., Heidelberg (Germany)
  8. The Jackson Lab. for Genomic Medicine, Farmington, CT (United States)
  9. Michigan State Univ., East Lansing, MI (United States)
  10. California State Univ., San Bernardino, CA (United States)
  11. J. Craig Venter Institute, San Diego, CA (United States)
  12. J. Craig Venter Institute, Rockville, MD (United States)
  13. Max Planck Institute for Marine Microbiology, Bremen (Germany)
  14. Univ. of Chicago, Chicago, IL (United States)
  15. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  16. Univ. of British Columbia, Vancouver, BC (Canada)
  17. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of Southern California, Los Angeles, CA (United States)
  18. Uppsala Univ., Uppsala (Sweden)
  19. Univ. of Vermont Cancer Center, Burlington, VT (United States)
  20. Georgia Inst. of Technology, Atlanta, GA (United States)
  21. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  22. Univ. of Texas-Austin, Austin, TX (United States)
  23. Univ. of Vienna, Vienna (Austria)
  24. Univ. of California, Davis, CA (United States)
  25. Univ. of Nevada, Las Vegas, NV (United States)
  26. Univ. of Wisconsin, Madison, WI (United States)
  27. Univ. of Colorado, Boulder, CO (United States)
  28. Univ. of California San Diego, La Jolla, CA (United States)
  29. European Molecular Biology Lab., Cambridge (United Kingdom)
  30. National Institutes of Health, Bethesda, MD (United States)
  31. The Univ. of Queensland, Queensland (Australia)
  32. National Cancer Institute, Frederick, MD (United States)
  33. Argonne National Lab. (ANL), Argonne, IL (United States)
  34. Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, Chicago, IL (United States)
  35. St. Petersburg State Univ., St. Petersburg (Russia)
  36. Knapp Center for Biomedical Discovery, Chicago, IL (United States)
  37. Univ. of California, Berkeley, CA (United States)
Publication Date:
Report Number(s):
PNNL-SA-125978
Journal ID: ISSN 1087-0156; 137394
Grant/Contract Number:
AC02-06CH11357; AC05-00OR22725; AC05-76RL01830; AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Nature Biotechnology
Additional Journal Information:
Journal Volume: 35; Journal Issue: 8; Journal ID: ISSN 1087-0156
Publisher:
Springer Nature
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
OSTI Identifier:
1375044
Alternate Identifier(s):
OSTI ID: 1394303; OSTI ID: 1414520; OSTI ID: 1459393