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Title: Intracellular distribution and stability of a luminescent rhenium(I) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

Optical fluorescence microscopy was used in conjunction with X-ray fluorescence microscopy to monitor the stability and intracellular distribution of the luminescent rhenium(I) complex fac-[Re(CO) 3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex, in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence techniques. Furthermore, X-ray fluorescence also showed that the Re-I complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.
Authors:
 [1] ; ORCiD logo [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [2] ;  [5] ;  [5] ;  [4] ;  [4] ;  [6] ;  [7]
  1. The Univ. of Adelaide (Australia)
  2. Univ. of South Australia, Adelaide (Australia)
  3. The Univ. of Sydney (Australia)
  4. Dept. of Chemistry, Perth (Australia)
  5. Argonne National Lab. (ANL), Argonne, IL (United States)
  6. Univ. of Western Australia, Crawley (Australia)
  7. Univ. of Bologna, Bologna (Italy)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
Metallomics
Additional Journal Information:
Journal Volume: 9; Journal Issue: 4; Journal ID: ISSN 1756-5901
Publisher:
Royal Society of Chemistry
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
Australian Research Council; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
OSTI Identifier:
1374605