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Title: Multifunctional, Tunable Metal–Organic Framework Materials Platform for Bioimaging Applications [A Multifunctional Tunable MOF Materials Platform for Bio-Imaging Applications]

Herein, we describe a novel multifunctional metal–organic framework (MOF) materials platform that displays both porosity and tunable emission properties as a function of the metal identity (Eu, Nd, and tuned compositions of Nd/Yb). Their emission collectively spans the deep red to near-infrared (NIR) spectral region (~614–1350 nm), which is highly relevant for in vivo bioimaging. These new materials meet important prerequisites as relevant to biological processes: they are minimally toxic to living cells and retain structural integrity in water and phosphate-buffered saline. To assess their viability as optical bioimaging agents, we successfully synthesized the nanoscale Eu analog as a proof-of-concept system in this series. In vitro studies show that it is cell-permeable in individual RAW 264.7 mouse macrophage and HeLa human cervical cancer tissue culture cells. The efficient discrimination between the Eu emission and cell autofluorescence was achieved with hyperspectral confocal fluorescence microscopy, used here for the first time to characterize MOF materials. Importantly, this is the first report that documents the long-term conservation of the intrinsic emission in live cells of a fluorophore-based MOF to date (up to 48 h). As a result this finding, in conjunction with the materials’ very low toxicity, validates the biocompatibility in these systemsmore » and qualifies them as promising for use in long-term tracking and biodistribution studies.« less
Authors:
ORCiD logo [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
ACS Applied Materials and Interfaces
Additional Journal Information:
Journal Volume: 9; Journal Issue: 27; Journal ID: ISSN 1944-8244
Publisher:
American Chemical Society (ACS)
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22), Scientific User Facilities Division
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; metal-organic framework; bio-imaging; emission; lanthanide; near-infrared
OSTI Identifier:
1373947

Sava Gallis, Dorina F., Rohwer, Lauren E. S., Rodriguez, Mark A., Barnhart-Dailey, Meghan C., Butler, Kimberly S., Luk, Ting S., Timlin, Jerilyn A., and Chapman, Karena W.. Multifunctional, Tunable Metal–Organic Framework Materials Platform for Bioimaging Applications [A Multifunctional Tunable MOF Materials Platform for Bio-Imaging Applications]. United States: N. p., Web. doi:10.1021/acsami.7b05859.
Sava Gallis, Dorina F., Rohwer, Lauren E. S., Rodriguez, Mark A., Barnhart-Dailey, Meghan C., Butler, Kimberly S., Luk, Ting S., Timlin, Jerilyn A., & Chapman, Karena W.. Multifunctional, Tunable Metal–Organic Framework Materials Platform for Bioimaging Applications [A Multifunctional Tunable MOF Materials Platform for Bio-Imaging Applications]. United States. doi:10.1021/acsami.7b05859.
Sava Gallis, Dorina F., Rohwer, Lauren E. S., Rodriguez, Mark A., Barnhart-Dailey, Meghan C., Butler, Kimberly S., Luk, Ting S., Timlin, Jerilyn A., and Chapman, Karena W.. 2017. "Multifunctional, Tunable Metal–Organic Framework Materials Platform for Bioimaging Applications [A Multifunctional Tunable MOF Materials Platform for Bio-Imaging Applications]". United States. doi:10.1021/acsami.7b05859. https://www.osti.gov/servlets/purl/1373947.
@article{osti_1373947,
title = {Multifunctional, Tunable Metal–Organic Framework Materials Platform for Bioimaging Applications [A Multifunctional Tunable MOF Materials Platform for Bio-Imaging Applications]},
author = {Sava Gallis, Dorina F. and Rohwer, Lauren E. S. and Rodriguez, Mark A. and Barnhart-Dailey, Meghan C. and Butler, Kimberly S. and Luk, Ting S. and Timlin, Jerilyn A. and Chapman, Karena W.},
abstractNote = {Herein, we describe a novel multifunctional metal–organic framework (MOF) materials platform that displays both porosity and tunable emission properties as a function of the metal identity (Eu, Nd, and tuned compositions of Nd/Yb). Their emission collectively spans the deep red to near-infrared (NIR) spectral region (~614–1350 nm), which is highly relevant for in vivo bioimaging. These new materials meet important prerequisites as relevant to biological processes: they are minimally toxic to living cells and retain structural integrity in water and phosphate-buffered saline. To assess their viability as optical bioimaging agents, we successfully synthesized the nanoscale Eu analog as a proof-of-concept system in this series. In vitro studies show that it is cell-permeable in individual RAW 264.7 mouse macrophage and HeLa human cervical cancer tissue culture cells. The efficient discrimination between the Eu emission and cell autofluorescence was achieved with hyperspectral confocal fluorescence microscopy, used here for the first time to characterize MOF materials. Importantly, this is the first report that documents the long-term conservation of the intrinsic emission in live cells of a fluorophore-based MOF to date (up to 48 h). As a result this finding, in conjunction with the materials’ very low toxicity, validates the biocompatibility in these systems and qualifies them as promising for use in long-term tracking and biodistribution studies.},
doi = {10.1021/acsami.7b05859},
journal = {ACS Applied Materials and Interfaces},
number = 27,
volume = 9,
place = {United States},
year = {2017},
month = {6}
}