skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Harmonization of experimental approach and data collection to streamline analysis of biomass composition from algae in an inter-laboratory setting

Abstract

In order to establish and design long-term algae cultivation experiments, inter-laboratory projects need to harmonize the requirements of techno-economic and life-cycle analysis models, with standardized data inputs. In order to provide a consistent foundation and allow for integration and analysis of the results in computational technical and resource analysis models, we implemented closely coordinated, harmonized and objective analytical protocols along with a common language for measuring growth and productivity for the major algal components. We describe here the process by which we developed a harmonization framework for analysis across five geographically diverse testbed sites. Our goal was to align analytical procedures to ensure consistent reporting on biomass and lipid content, quality and yields to eliminate measurement variability as a source of uncertainty in production data. Developing standards for analysis that streamline reporting on composition and expected fuel yields from biomass is one of the major outcomes of this work and this provides a starting place for further advanced characterization of algae to support the techno-economical process analyses and account for the mass balance accounting of algal biomass. Initial analysis of data obtained from field studies shows trends in compositional shifts of lipid and protein content of the biomass that aremore » in support of the physiological experiments demonstrated in the first geographically distributed unified outdoor cultivation trials.« less

Authors:
 [1];  [1];  [1];  [2];  [3]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Harmon Consulting, Kamuela, HI (United States)
  3. Arizona State Univ., Mesa, AZ (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Arizona State Univ., Tempe, AZ (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1373264
Alternate Identifier(s):
OSTI ID: 1550511; OSTI ID: 1785543
Report Number(s):
NREL/JA-5100-68598
Journal ID: ISSN 2211-9264
Grant/Contract Number:  
AC36-08GO28308; EE0005996; AC36-08-GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 25; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; algae; biomass; composition; lipids; carbohydrates; protein; harmonization; Algae, Biomass, Composition, Lipids, Carbohydrates, Protein, Harmonization

Citation Formats

Laurens, Lieve M. L., Van Wychen, Stefanie, Pienkos, Philip T., Harmon, Valerie L., and McGowen, John. Harmonization of experimental approach and data collection to streamline analysis of biomass composition from algae in an inter-laboratory setting. United States: N. p., 2017. Web. https://doi.org/10.1016/j.algal.2017.03.029.
Laurens, Lieve M. L., Van Wychen, Stefanie, Pienkos, Philip T., Harmon, Valerie L., & McGowen, John. Harmonization of experimental approach and data collection to streamline analysis of biomass composition from algae in an inter-laboratory setting. United States. https://doi.org/10.1016/j.algal.2017.03.029
Laurens, Lieve M. L., Van Wychen, Stefanie, Pienkos, Philip T., Harmon, Valerie L., and McGowen, John. Wed . "Harmonization of experimental approach and data collection to streamline analysis of biomass composition from algae in an inter-laboratory setting". United States. https://doi.org/10.1016/j.algal.2017.03.029. https://www.osti.gov/servlets/purl/1373264.
@article{osti_1373264,
title = {Harmonization of experimental approach and data collection to streamline analysis of biomass composition from algae in an inter-laboratory setting},
author = {Laurens, Lieve M. L. and Van Wychen, Stefanie and Pienkos, Philip T. and Harmon, Valerie L. and McGowen, John},
abstractNote = {In order to establish and design long-term algae cultivation experiments, inter-laboratory projects need to harmonize the requirements of techno-economic and life-cycle analysis models, with standardized data inputs. In order to provide a consistent foundation and allow for integration and analysis of the results in computational technical and resource analysis models, we implemented closely coordinated, harmonized and objective analytical protocols along with a common language for measuring growth and productivity for the major algal components. We describe here the process by which we developed a harmonization framework for analysis across five geographically diverse testbed sites. Our goal was to align analytical procedures to ensure consistent reporting on biomass and lipid content, quality and yields to eliminate measurement variability as a source of uncertainty in production data. Developing standards for analysis that streamline reporting on composition and expected fuel yields from biomass is one of the major outcomes of this work and this provides a starting place for further advanced characterization of algae to support the techno-economical process analyses and account for the mass balance accounting of algal biomass. Initial analysis of data obtained from field studies shows trends in compositional shifts of lipid and protein content of the biomass that are in support of the physiological experiments demonstrated in the first geographically distributed unified outdoor cultivation trials.},
doi = {10.1016/j.algal.2017.03.029},
journal = {Algal Research},
number = C,
volume = 25,
place = {United States},
year = {2017},
month = {4}
}

Journal Article:

Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

A mechanistic model for describing dynamic multi-nutrient, light, temperature interactions in phytoplankton
journal, September 2001


Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics
journal, January 2010

  • Williams, Peter J. le B.; Laurens, Lieve M. L.
  • Energy & Environmental Science, Vol. 3, Issue 5
  • DOI: 10.1039/b924978h

Integrated Evaluation of Cost, Emissions, and Resource Potential for Algal Biofuels at the National Scale
journal, May 2014

  • Davis, Ryan E.; Fishman, Daniel B.; Frank, Edward D.
  • Environmental Science & Technology, Vol. 48, Issue 10
  • DOI: 10.1021/es4055719

Siting Algae Cultivation Facilities for Biofuel Production in the United States: Trade-Offs between Growth Rate, Site Constructability, Water Availability, and Infrastructure
journal, March 2014

  • Venteris, Erik R.; McBride, Robert C.; Coleman, Andre M.
  • Environmental Science & Technology, Vol. 48, Issue 6
  • DOI: 10.1021/es4045488

Algal Biomass Constituent Analysis: Method Uncertainties and Investigation of the Underlying Measuring Chemistries
journal, January 2012

  • Laurens, Lieve M. L.; Dempster, Thomas A.; Jones, Howland D. T.
  • Analytical Chemistry, Vol. 84, Issue 4
  • DOI: 10.1021/ac202668c

Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition
journal, May 2014

  • Laurens, Lieve M. L.; Van Wychen, Stefanie; McAllister, Jordan P.
  • Analytical Biochemistry, Vol. 452
  • DOI: 10.1016/j.ab.2014.02.009

Patterns of carbohydrate and fatty acid changes under nitrogen starvation in the microalgae Haematococcus pluvialis and Nannochloropsis sp.
journal, February 2012

  • Recht, Lee; Zarka, Aliza; Boussiba, Sammy
  • Applied Microbiology and Biotechnology, Vol. 94, Issue 6
  • DOI: 10.1007/s00253-012-3940-4

Assessment of key biological and engineering design parameters for production of Chlorella zofingiensis (Chlorophyceae) in outdoor photobioreactors
journal, May 2013

  • Zemke, Peter E.; Sommerfeld, Milton R.; Hu, Qiang
  • Applied Microbiology and Biotechnology, Vol. 97, Issue 12
  • DOI: 10.1007/s00253-013-4919-5

A flexible culture process for production of the green microalga Scenedesmus dimorphus rich in protein, carbohydrate or lipid
journal, February 2013


Effect of Nutrient Limitation on Fatty acid and Lipid Content of Marine Microalgae1
journal, December 1994


Effects of nutrient and light limitation on the biochemical composition of phytoplankton
journal, March 1990

  • Harrison, P. J.; Thompson, P. A.; Calderwood, G. S.
  • Journal of Applied Phycology, Vol. 2, Issue 1
  • DOI: 10.1007/BF02179768

MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates
journal, February 2017


Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification
journal, February 2012

  • Laurens, Lieve M. L.; Quinn, Matthew; Van Wychen, Stefanie
  • Analytical and Bioanalytical Chemistry, Vol. 403, Issue 1
  • DOI: 10.1007/s00216-012-5814-0

Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors
journal, February 2004

  • Lourenço, Sergio O.; Barbarino, Elisabete; Lavín, Paris L.
  • European Journal of Phycology, Vol. 39, Issue 1
  • DOI: 10.1080/0967026032000157156

    Works referencing / citing this record:

    Unified field studies of the algae testbed public-private partnership as the benchmark for algae agronomics
    journal, November 2018

    • Knoshaug, Eric P.; Wolfrum, Ed; Laurens, Lieve M. L.
    • Scientific Data, Vol. 5, Issue 1
    • DOI: 10.1038/sdata.2018.267

    Development of algae biorefinery concepts for biofuels and bioproducts; a perspective on process-compatible products and their impact on cost-reduction
    journal, January 2017

    • Laurens, Lieve M. L.; Markham, Jennifer; Templeton, David W.
    • Energy & Environmental Science, Vol. 10, Issue 8
    • DOI: 10.1039/c7ee01306j