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Title: Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.
 [1] ;  [1] ;  [2] ; ORCiD logo [1]
  1. Georg-August-Univ., Gottingen (Germany). Inst. for X-ray Physics
  2. Max Planck Inst. for Experimental Medicine and Univ. Medical Center, Gottingen (Germany)
Publication Date:
Report Number(s):
Journal ID: ISSN 2053-2733; ACSAD7; R&D Project: PO011; KC0201060
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section A, Foundations and Advances (Online)
Additional Journal Information:
Journal Name: Acta Crystallographica. Section A, Foundations and Advances (Online); Journal Volume: 73; Journal Issue: 4; Journal ID: ISSN 2053-2733
International Union of Crystallography
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; x-ray holography; phase retrieval; x-ray tomography; x-ray waveguides; coherent imaging
OSTI Identifier: