skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

Abstract

X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.

Authors:
 [1];  [1];  [2]; ORCiD logo [1]
  1. Georg-August-Univ., Gottingen (Germany). Inst. for X-ray Physics
  2. Max Planck Inst. for Experimental Medicine and Univ. Medical Center, Gottingen (Germany)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1372448
Report Number(s):
BNL-114046-2017-JA
Journal ID: ISSN 2053-2733; ACSAD7; R&D Project: PO011; KC0201060
Grant/Contract Number:  
SC00112704
Resource Type:
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section A, Foundations and Advances (Online)
Additional Journal Information:
Journal Name: Acta Crystallographica. Section A, Foundations and Advances (Online); Journal Volume: 73; Journal Issue: 4; Journal ID: ISSN 2053-2733
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; x-ray holography; phase retrieval; x-ray tomography; x-ray waveguides; coherent imaging

Citation Formats

Krenkel, Martin, Toepperwien, Mareike, Alves, Frauke, and Salditt, Tim. Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime. United States: N. p., 2017. Web. doi:10.1107/S2053273317007902.
Krenkel, Martin, Toepperwien, Mareike, Alves, Frauke, & Salditt, Tim. Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime. United States. doi:10.1107/S2053273317007902.
Krenkel, Martin, Toepperwien, Mareike, Alves, Frauke, and Salditt, Tim. Thu . "Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime". United States. doi:10.1107/S2053273317007902. https://www.osti.gov/servlets/purl/1372448.
@article{osti_1372448,
title = {Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime},
author = {Krenkel, Martin and Toepperwien, Mareike and Alves, Frauke and Salditt, Tim},
abstractNote = {X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.},
doi = {10.1107/S2053273317007902},
journal = {Acta Crystallographica. Section A, Foundations and Advances (Online)},
number = 4,
volume = 73,
place = {United States},
year = {2017},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 6 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

X-Ray Holographic Imaging of Hydrated Biological Cells in Solution
journal, January 2015


Low-dose three-dimensional hard x-ray imaging of bacterial cells
journal, January 2012

  • Bartels, Matthias; Priebe, Marius; Wilke, Robin N.
  • Optical Nanoscopy, Vol. 1, Issue 1
  • DOI: 10.1186/2192-2853-1-10

Phase retrieval in X-ray phase-contrast imaging suitable for tomography
journal, January 2011

  • Burvall, Anna; Lundström, Ulf; Takman, Per A. C.
  • Optics Express, Vol. 19, Issue 11
  • DOI: 10.1364/OE.19.010359

Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network
journal, September 2006

  • Cloetens, P.; Mache, R.; Schlenker, M.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 39
  • DOI: 10.1073/pnas.0603490103

Phase retrieval algorithms: a comparison
journal, January 1982


A New Microscopic Principle
journal, May 1948


Single-shot Femtosecond X-Ray Holography Using Extended References
journal, August 2010


Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging
journal, November 2015


X-ray propagation microscopy of biological cells using waveguides as a quasipoint source
journal, February 2011


Holographic and diffractive x-ray imaging using waveguides as quasi-point sources
journal, March 2010


Algebraic Reconstruction Techniques (ART) for three-dimensional electron microscopy and X-ray photography
journal, December 1970


Quantitative interior x-ray nanotomography by a hybrid imaging technique
journal, January 2015


Optical phase retrieval by use of first Born- and Rytov-type approximations
journal, January 2004

  • Gureyev, Timur E.; Davis, Timothy J.; Pogany, Andrew
  • Applied Optics, Vol. 43, Issue 12
  • DOI: 10.1364/AO.43.002418

Reconstruction of wave front and object for inline holography from a set of detection planes
journal, January 2014

  • Hagemann, J.; Robisch, A. -L.; Luke, D. R.
  • Optics Express, Vol. 22, Issue 10
  • DOI: 10.1364/OE.22.011552

Signal-to-noise and radiation exposure considerations in conventional and diffraction x-ray microscopy
journal, January 2009

  • Huang, Xiaojing; Miao, Huijie; Steinbrener, Jan
  • Optics Express, Vol. 17, Issue 16
  • DOI: 10.1364/OE.17.013541

Transport of intensity phase reconstruction to solve the twin image problem in holographic x-ray imaging
journal, January 2013


Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs
journal, May 2015

  • Krenkel, Martin; Markus, Andrea; Bartels, Matthias
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep09973

Sub-10 nm beam confinement by X-ray waveguides: design, fabrication and characterization of optical properties
journal, January 2012

  • Krüger, S. P.; Neubauer, H.; Bartels, M.
  • Journal of Synchrotron Radiation, Vol. 19, Issue 2
  • DOI: 10.1107/S0909049511051983

Quantitative comparison of direct phase retrieval algorithms in in-line phase tomography: Quantitative comparison of direct phase retrieval algorithms
journal, September 2008

  • Langer, Max; Cloetens, Peter; Guigay, Jean-Pierre
  • Medical Physics, Vol. 35, Issue 10
  • DOI: 10.1118/1.2975224

Imaging cellular architecture with X-rays
journal, October 2010

  • Larabell, Carolyn A.; Nugent, Keith A.
  • Current Opinion in Structural Biology, Vol. 20, Issue 5
  • DOI: 10.1016/j.sbi.2010.08.008

Solution to the Twin Image Problem in Holography
journal, June 2007


Cryogenic X-Ray Diffraction Microscopy for Biological Samples
journal, November 2009


Regularized Newton methods for x-ray phase contrast and general imaging problems
journal, January 2016

  • Maretzke, Simon; Bartels, Matthias; Krenkel, Martin
  • Optics Express, Vol. 24, Issue 6
  • DOI: 10.1364/OE.24.006490

Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens
journal, July 1999

  • Miao, Jianwei; Charalambous, Pambos; Kirz, Janos
  • Nature, Vol. 400, Issue 6742
  • DOI: 10.1038/22498

Nonlinear phase retrieval from single-distance radiograph
journal, January 2010

  • Moosmann, Julian; Hofmann, Ralf; Bronnikov, Andrei
  • Optics Express, Vol. 18, Issue 25
  • DOI: 10.1364/OE.18.025771

Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
journal, January 2009


Coherence filtering of x-ray waveguides: analytical and numerical approach
journal, October 2011


Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object
journal, April 2002


Noninterferometric Phase Imaging with Partially Coherent Light
journal, March 1998


Near-field ptychography using lateral and longitudinal shifts
journal, July 2015


Three-dimensional phase retrieval in propagation-based phase-contrast imaging
journal, March 2014


Three-dimensional propagation in near-field tomographic X-ray phase retrieval
journal, January 2016

  • Ruhlandt, Aike; Salditt, Tim
  • Acta Crystallographica Section A Foundations and Advances, Vol. 72, Issue 2
  • DOI: 10.1107/S2053273315022469

Compound focusing mirror and X-ray waveguide optics for coherent imaging and nano-diffraction
journal, June 2015

  • Salditt, Tim; Osterhoff, Markus; Krenkel, Martin
  • Journal of Synchrotron Radiation, Vol. 22, Issue 4
  • DOI: 10.1107/S1600577515007742

Biological imaging by soft x-ray diffraction microscopy
journal, October 2005

  • Shapiro, D.; Thibault, P.; Beetz, T.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 43
  • DOI: 10.1073/pnas.0503305102

Quantitative Imaging of Single, Unstained Viruses with Coherent X Rays
journal, October 2008


X-ray nanotomography using near-field ptychography
journal, January 2015

  • Stockmar, Marco; Hubert, Maxime; Dierolf, Martin
  • Optics Express, Vol. 23, Issue 10
  • DOI: 10.1364/OE.23.012720

Deterministic phase retrieval: a Green’s function solution
journal, January 1983

  • Teague, Michael Reed
  • Journal of the Optical Society of America, Vol. 73, Issue 11
  • DOI: 10.1364/JOSA.73.001434

High-Resolution Scanning X-ray Diffraction Microscopy
journal, July 2008


Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT
conference, October 2016

  • Töpperwien, Mareike; Krenkel, Martin; Müller, Kristin
  • SPIE Optical Engineering + Applications, SPIE Proceedings
  • DOI: 10.1117/12.2238496

X-ray phase imaging: Demonstration of extended conditions with homogeneous objects
journal, January 2004


Transport of Intensity imaging with higher order derivatives
journal, January 2010

  • Waller, Laura; Tian, Lei; Barbastathis, George
  • Optics Express, Vol. 18, Issue 12
  • DOI: 10.1364/OE.18.012552

Quantitative X-ray phase contrast waveguide imaging of bacterial endospores
journal, March 2015


Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction
journal, January 2012


Optimization of phase contrast imaging using hard x rays
journal, July 2005

  • Zabler, S.; Cloetens, P.; Guigay, J. -P.
  • Review of Scientific Instruments, Vol. 76, Issue 7
  • DOI: 10.1063/1.1960797

    Works referencing / citing this record:

    Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
    text, January 2017

    • Krenkel, Martin; Toepperwien, Mareike; Alves, Frauke
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2017-11602

    A beamline-compatible STED microscope for combined visible-light and X-ray studies of biological matter
    journal, May 2019

    • Bernhardt, Marten; Nicolas, Jan-David; Osterhoff, Markus
    • Journal of Synchrotron Radiation, Vol. 26, Issue 4
    • DOI: 10.1107/s1600577519004089

    Phase retrieval for near-field X-ray imaging beyond linearisation or compact support
    journal, July 2018

    • Hagemann, J.; Töpperwien, M.; Salditt, T.
    • Applied Physics Letters, Vol. 113, Issue 4
    • DOI: 10.1063/1.5029927

    Phase retrieval for near-field X-ray imaging beyond linearisation or compact support
    journal, July 2018

    • Hagemann, J.; Töpperwien, M.; Salditt, T.
    • Applied Physics Letters, Vol. 113, Issue 4
    • DOI: 10.1063/1.5029927

    A beamline-compatible STED microscope for combined visible-light and X-ray studies of biological matter
    journal, May 2019

    • Bernhardt, Marten; Nicolas, Jan-David; Osterhoff, Markus
    • Journal of Synchrotron Radiation, Vol. 26, Issue 4
    • DOI: 10.1107/s1600577519004089

    Evaluation of different heavy-metal stains and embedding media for phase contrast tomography of neuronal tissue
    conference, September 2019

    • Eckermann, Marina; Töpperwien, Mareike; Ruhwedel, Torben
    • Developments in X-Ray Tomography XII
    • DOI: 10.1117/12.2528432