An Enzyme-Free Signal Amplification Technique for Ultrasensitive Colorimetric Assay of Disease Biomarkers

Ye, Haihang; Yang, Kuikun; Tao, Jing; Liu, Yijing; Zhang, Qian; Habibi, Sanaz; Nie, Zhihong; Xia, Xiaohu

doi:10.1021/acsnano.6b08232

Title: An Enzyme-Free Signal Amplification Technique for Ultrasensitive Colorimetric Assay of Disease Biomarkers

Journal Article · 29 January 2017 · ACS Nano

DOI: https://doi.org/10.1021/acsnano.6b08232 · OSTI ID:1372434

Ye, Haihang ^[1]; Yang, Kuikun ^[2]; Tao, Jing ^[3]; Liu, Yijing ^[2]; Zhang, Qian ^[2]; Habibi, Sanaz ^[4];

^[2];

^[1]

Michigan Technological Univ., Houghton, MI (United States). Dept. of Chemistry
Univ. of Maryland, College Park, MD (United States). Dept. of Chemistry and Biochemistry
Brookhaven National Lab. (BNL), Upton, NY (United States). Condensed Matter Physics and Materials Science Dept.
Brookhaven National Lab. (BNL), Upton, NY (United States). Condensed Matter Physics and Materials Science Dept.; Michigan Technological Univ., Houghton, MI (United States). Dept. of Chemical Engineering

Enzyme-based colorimetric assays have been widely used in research labs and clinical diagnosis for decades. Nevertheless, as constrained by the performance of enzymes, their detection sensitivity has not been substantially improved in recent years, which inhibits many critical applications such as early detection of cancers. In this work, we demonstrate an enzyme-free signal amplification technique, based on gold vesicles encapsulated with Pd-Ir nanoparticles as peroxidase mimics, for colorimetric assay of disease biomarkers with significantly enhanced sensitivity. This technique overcomes the intrinsic limitations of enzymes, thanks to the superior catalytic efficiency of peroxidase mimics and the efficient loading and release of these mimics. Using human prostate surface antigen as a model biomarker, we demonstrated that the enzyme-free assay could reach a limit of detection at the femtogram/mL level, which is over 10³-fold lower than that of conventional enzyme-based assay when the same antibodies and similar procedure were used.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0012704

OSTI ID:: 1372434

Report Number(s):: BNL--113910-2017-JA; KC0201010

Journal Information:: ACS Nano, Journal Name: ACS Nano Journal Issue: 2 Vol. 11; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Title: An Enzyme-Free Signal Amplification Technique for Ultrasensitive Colorimetric Assay of Disease Biomarkers

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