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Title: Characterization of zein assemblies by ultra-small-angle X-ray scattering

Abstract

Zein, a protein of corn, has an amphiphilic molecule capable of self-assembly into distinctly different structures. In this work, ultra-small-angle X-ray scattering (USAXS) was applied to investigate the formation of self-assembled zein structures in binary solvent systems of ethanol and water. Our study included observing structural changes due to aging. Three hierarchical structures were identified in zein-solvent systems, molecular zein 2D films, believed to be formed by zein rods assembled first into one-dimensional fibers and then into two-dimensional films, and 3D spherical aggregates. Aging did not change the size or shape of primary units, but promoted their self-assembly into intermediate 2D structures and shaped 3D structures into well19 defined spheres. We found that the rheological parameters, consistency index (K) and behavior index (n), storage and loss moduli (G’ and G”) were also measured. K and n, changed markedly with aging, from nearly Newtonian low consistency fresh samples to highly viscous pseudoplastic aged samples. G’ and G” increased with aging for all samples reflecting increased interactions among zein self-assembled structures. Furthermore, viscoelastic parameters indicated that zein dispersions formed gels upon aging. It was observed that USAX reported on molecular scale self-assembly processes, while rheological measurements reported on the macroscale interaction betweenmore » self-assembled particles. Raman spectra suggested that α-helix to β-sheet transformations prompted zein self-assembly, which influenced the size and morphology of molecular assemblies and ultimately the rheological properties of zein dispersions.« less

Authors:
; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); U.S. Department of Agriculture (USDA) - National Institute of Food and Agriculture (NIFA)
OSTI Identifier:
1372296
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Soft Matter
Additional Journal Information:
Journal Volume: 13; Journal Issue: 16; Journal ID: ISSN 1744-683X
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; USAXS; aging; hierarchical nanostructures; self-assembly; zein

Citation Formats

Uzun, Suzan, Ilavsky, Jan, and Padua, Graciela Wild. Characterization of zein assemblies by ultra-small-angle X-ray scattering. United States: N. p., 2017. Web. doi:10.1039/c6sm02717b.
Uzun, Suzan, Ilavsky, Jan, & Padua, Graciela Wild. Characterization of zein assemblies by ultra-small-angle X-ray scattering. United States. doi:10.1039/c6sm02717b.
Uzun, Suzan, Ilavsky, Jan, and Padua, Graciela Wild. Thu . "Characterization of zein assemblies by ultra-small-angle X-ray scattering". United States. doi:10.1039/c6sm02717b. https://www.osti.gov/servlets/purl/1372296.
@article{osti_1372296,
title = {Characterization of zein assemblies by ultra-small-angle X-ray scattering},
author = {Uzun, Suzan and Ilavsky, Jan and Padua, Graciela Wild},
abstractNote = {Zein, a protein of corn, has an amphiphilic molecule capable of self-assembly into distinctly different structures. In this work, ultra-small-angle X-ray scattering (USAXS) was applied to investigate the formation of self-assembled zein structures in binary solvent systems of ethanol and water. Our study included observing structural changes due to aging. Three hierarchical structures were identified in zein-solvent systems, molecular zein 2D films, believed to be formed by zein rods assembled first into one-dimensional fibers and then into two-dimensional films, and 3D spherical aggregates. Aging did not change the size or shape of primary units, but promoted their self-assembly into intermediate 2D structures and shaped 3D structures into well19 defined spheres. We found that the rheological parameters, consistency index (K) and behavior index (n), storage and loss moduli (G’ and G”) were also measured. K and n, changed markedly with aging, from nearly Newtonian low consistency fresh samples to highly viscous pseudoplastic aged samples. G’ and G” increased with aging for all samples reflecting increased interactions among zein self-assembled structures. Furthermore, viscoelastic parameters indicated that zein dispersions formed gels upon aging. It was observed that USAX reported on molecular scale self-assembly processes, while rheological measurements reported on the macroscale interaction between self-assembled particles. Raman spectra suggested that α-helix to β-sheet transformations prompted zein self-assembly, which influenced the size and morphology of molecular assemblies and ultimately the rheological properties of zein dispersions.},
doi = {10.1039/c6sm02717b},
journal = {Soft Matter},
number = 16,
volume = 13,
place = {United States},
year = {2017},
month = {3}
}

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Works referenced in this record:

Nanoscale Characterization of Zein Self-Assembly
journal, January 2012


Studies on egg albumen and whey protein interactions by FT-Raman spectroscopy and rheology
journal, January 2004


Supramolecular Nanostructure Formation of Coassembled Amyloid Inspired Peptides
journal, June 2016


Effect of Hydrophilic and Lipophilic Compounds on Zein Microstructures
journal, April 2008


Production and characterization of polycaprolactone- hyaluronic acid/chitosan- zein electrospun bilayer nanofibrous membrane for tissue regeneration
journal, December 2016

  • Figueira, Daniela R.; Miguel, Sónia P.; de Sá, Kevin D.
  • International Journal of Biological Macromolecules, Vol. 93
  • DOI: 10.1016/j.ijbiomac.2016.09.080

In-situ synthesis of AgNPs in the natural/synthetic hybrid nanofibrous scaffolds: Fabrication, characterization and antimicrobial activities
journal, January 2017

  • Maharjan, Bikendra; Joshi, Mahesh Kumar; Tiwari, Arjun Prasad
  • Journal of the Mechanical Behavior of Biomedical Materials, Vol. 65
  • DOI: 10.1016/j.jmbbm.2016.07.034

Dual responsive zein hydrogel membrane with selective protein adsorption and sustained release property
journal, January 2017


Three-dimensional structure of maize α-zein proteins studied by small-angle X-ray scattering
journal, April 1997

  • Matsushima, Norio; Danno, Gen-ichi; Takezawa, Hikaru
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1339, Issue 1
  • DOI: 10.1016/S0167-4838(96)00212-9

X-ray diffraction characterization of the structure of zein-Oleic acid films
journal, February 1999


Raman Spectroscopic Characterization of Secondary Structure in Natively Unfolded Proteins:  α-Synuclein
journal, March 2004

  • Maiti, Nakul C.; Apetri, Mihaela M.; Zagorski, Michael G.
  • Journal of the American Chemical Society, Vol. 126, Issue 8
  • DOI: 10.1021/ja0356176

Supramolecular Hydrogel of a d -Amino Acid Dipeptide for Controlled Drug Release in Vivo
journal, August 2009

  • Liang, Gaolin; Yang, Zhimou; Zhang, Rongjun
  • Langmuir, Vol. 25, Issue 15
  • DOI: 10.1021/la804271d

Raman spectroscopy of heat-induced fine-stranded and particulate β-lactoglobulin gels
journal, May 2004


Structural Investigation of Edible Zein Films/Coatings and Directly Determining Their Thickness by FT-Raman Spectroscopy
journal, June 2005

  • Hsu, Bao-Lian; Weng, Yih-Ming; Liao, Yu-Hsiu
  • Journal of Agricultural and Food Chemistry, Vol. 53, Issue 13
  • DOI: 10.1021/jf0501490

Cellular Uptake and Transport of Zein Nanoparticles: Effects of Sodium Caseinate
journal, July 2013

  • Luo, Yangchao; Teng, Zi; Wang, Thomas T. Y.
  • Journal of Agricultural and Food Chemistry, Vol. 61, Issue 31
  • DOI: 10.1021/jf402198r

Trace Solvent as a Predominant Factor To Tune Dipeptide Self-Assembly
journal, January 2016


Ultra-Small-Angle X-ray Scattering Instrument at the Advanced Photon Source: History, Recent Development, and Current Status
journal, October 2012

  • Ilavsky, J.; Zhang, F.; Allen, A. J.
  • Metallurgical and Materials Transactions A, Vol. 44, Issue 1
  • DOI: 10.1007/s11661-012-1431-y

Aggregate formation of zein and its structural inversion in aqueous ethanol
journal, January 2008


Cyclic compressive creep-elastoplastic behaviors of in situ TiB2/Al-reinforced composite
journal, June 2016


Rheology of Zein Solutions in Aqueous Ethanol
journal, May 1999

  • Fu, Dejing; Weller, Curtis L.
  • Journal of Agricultural and Food Chemistry, Vol. 47, Issue 5
  • DOI: 10.1021/jf9811121

Irena : tool suite for modeling and analysis of small-angle scattering
journal, February 2009


Triglyceride nanocrystal aggregation into polycrystalline colloidal networks: Ultra-small angle X-ray scattering, models and computer simulation
journal, October 2014

  • Peyronel, Fernanda; Pink, David A.; Marangoni, Alejandro G.
  • Current Opinion in Colloid & Interface Science, Vol. 19, Issue 5
  • DOI: 10.1016/j.cocis.2014.07.001

Zein: the industrial protein from corn
journal, May 2001


Self-assembling peptides form nanodiscs that stabilize membrane proteins
journal, January 2014

  • Midtgaard, Søren Roi; Pedersen, Martin Cramer; Kirkensgaard, Jacob Judas Kain
  • Soft Matter, Vol. 10, Issue 5
  • DOI: 10.1039/C3SM51727F

Effects of the environmental factors on the casein micelle structure studied by cryo transmission electron microscopy and small-angle x-ray scattering/ultrasmall-angle x-ray scattering
journal, January 2007

  • Marchin, Stéphane; Putaux, Jean-Luc; Pignon, Frédéric
  • The Journal of Chemical Physics, Vol. 126, Issue 4
  • DOI: 10.1063/1.2409933

Enzyme-Instructed Molecular Self-assembly Confers Nanofibers and a Supramolecular Hydrogel of Taxol Derivative
journal, September 2009

  • Gao, Yuan; Kuang, Yi; Guo, Zu-Feng
  • Journal of the American Chemical Society, Vol. 131, Issue 38
  • DOI: 10.1021/ja904411z

Understanding the Dissolution of α-Zein in Aqueous Ethanol and Acetic Acid Solutions
journal, September 2012

  • Li, Yunqi; Li, Ji; Xia, Qiuyang
  • The Journal of Physical Chemistry B, Vol. 116, Issue 39
  • DOI: 10.1021/jp305709y

Unraveling the Biomolecular Snapshots of Mitosis in Healthy and Cancer Cells Using Plasmonically-Enhanced Raman Spectroscopy
journal, November 2014

  • Panikkanvalappil, Sajanlal R.; Hira, Steven M.; Mahmoud, Mahmoud A.
  • Journal of the American Chemical Society, Vol. 136, Issue 45
  • DOI: 10.1021/ja506289u

Characterization of the nanoscale structure of milk fat
journal, July 2016