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Title: Population-level coordination of pigment response in individual cyanobacterial cells under altered nitrogen levels

Journal Article · · Photosynthesis Research
 [1]; ORCiD logo [2]; ORCiD logo [3];  [4]; ORCiD logo [5]; ORCiD logo [4]; ORCiD logo [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
  2. Washington Univ., St. Louis, MO (United States); Univ. of Colorado, Boulder, CO (United States)
  3. Washington Univ., St. Louis, MO (United States); United States Environmental Protection Agency, Washington, D.C. (United States)
  4. Washington Univ., St. Louis, MO (United States)
  5. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Old Dominion Univ., Norfolk, VA (United States)

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. We characterized the response of four key photosynthetic pigments to nitrogen depletion and repletion at the subcellular level in individual, live Synechocystis sp. PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. Our results revealed that PBS degradation and re-synthesis comprise a rapid response to nitrogen fluctuations, with coordinated populations of cells undergoing pigment modifications. Chlorophyll fluorescence originating from photosystem I and II decreased during nitrogen starvation, but no alteration in subcellular chlorophyll localization was found. Lastly, we observed differential rod and core pigment responses to nitrogen deprivation, suggesting that PBS complexes undergo a stepwise degradation process.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE
Grant/Contract Number:
AC04-94AL85000; SC0001035; NA0003525
OSTI ID:
1372115
Alternate ID(s):
OSTI ID: 1374747
Report Number(s):
SAND-2017-7775J; PII: 422
Journal Information:
Photosynthesis Research, Vol. 134, Issue 2; ISSN 0166-8595
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English

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