Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
Abstract
In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Furthermore, our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.
- Authors:
-
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), NREL Laboratory Directed Research and Development (LDRD)
- OSTI Identifier:
- 1372043
- Report Number(s):
- NREL/JA-2700-67215
Journal ID: ISSN 2045-2322
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; plant cell wall; recalcitrance; AcCel5A endoglucanase; biomass; in planta expression; bioalcohols; molecular engineering in plants
Citation Formats
Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., and Brunecky, Roman. Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase. United States: N. p., 2017.
Web. doi:10.1038/s41598-017-04502-1.
Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., & Brunecky, Roman. Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase. United States. https://doi.org/10.1038/s41598-017-04502-1
Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., and Brunecky, Roman. Thu .
"Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase". United States. https://doi.org/10.1038/s41598-017-04502-1. https://www.osti.gov/servlets/purl/1372043.
@article{osti_1372043,
title = {Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase},
author = {Donohoe, Bryon S. and Wei, Hui and Mittal, Ashutosh and Shollenberger, Todd and Lunin, Vladimir V. and Himmel, Michael E. and Brunecky, Roman},
abstractNote = {In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Furthermore, our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.},
doi = {10.1038/s41598-017-04502-1},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {Thu Jun 29 00:00:00 EDT 2017},
month = {Thu Jun 29 00:00:00 EDT 2017}
}
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