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Title: Complex biomembrane mimetics on the sub-nanometer scale

Biomimetic lipid vesicles are indispensable tools for gaining insight into the biophysics of cell physiology on the molecular level. The level of complexity of these model systems has steadily increased, and now spans from domain forming lipid mixtures to asymmetric lipid bilayers. We review recent progress in the development and application of elastic neutron and X-ray scattering techniques for studying these systems in situ and under physiologically relevant conditions on the nanometer to sub-nanometer length scales. Particularly we focus on: (i) structural details of coexisting liquid-ordered and liquid-disordered domains, including their thickness and lipid packing mismatch as a function of a size transition from nanoscopic to macroscopic domains; (ii) membrane-mediated protein partitioning into lipid domains; (iii) the role of the aqueous medium in tuning interactions between membranes and domains; and (iv) leaflet specific structure in asymmetric bilayers and passive lipid flip-flop.
 [1] ; ORCiD logo [2]
  1. Univ. of Tennessee, Knoxville, TN (United States). The Bredesen Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences, Biology and Soft Matter Division
  2. Univ. of Graz (Austria). Inst. of Molecular Biosciences, Soft Matter Division; BioTechMed, Graz (Austria)
Publication Date:
Grant/Contract Number:
Published Article
Journal Name:
Biophysical Reviews
Additional Journal Information:
Journal Volume: 9; Journal Issue: 4; Journal ID: ISSN 1867-2450
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; asymmetric bilayers; lipid domains; lipid flip-flog; lipid-protein interactions; intermembrane interactions; small-angle neutron and x-ray scattering
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1408573