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Title: Complex biomembrane mimetics on the sub-nanometer scale

Abstract

Biomimetic lipid vesicles are indispensable tools for gaining insight into the biophysics of cell physiology on the molecular level. The level of complexity of these model systems has steadily increased, and now spans from domain forming lipid mixtures to asymmetric lipid bilayers. We review recent progress in the development and application of elastic neutron and X-ray scattering techniques for studying these systems in situ and under physiologically relevant conditions on the nanometer to sub-nanometer length scales. Particularly we focus on: (i) structural details of coexisting liquid-ordered and liquid-disordered domains, including their thickness and lipid packing mismatch as a function of a size transition from nanoscopic to macroscopic domains; (ii) membrane-mediated protein partitioning into lipid domains; (iii) the role of the aqueous medium in tuning interactions between membranes and domains; and (iv) leaflet specific structure in asymmetric bilayers and passive lipid flip-flop.

Authors:
 [1]; ORCiD logo [2]
  1. Univ. of Tennessee, Knoxville, TN (United States). The Bredesen Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences, Biology and Soft Matter Division
  2. Univ. of Graz (Austria). Inst. of Molecular Biosciences, Soft Matter Division; BioTechMed, Graz (Austria)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1371488
Alternate Identifier(s):
OSTI ID: 1408573
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Biophysical Reviews
Additional Journal Information:
Journal Volume: 9; Journal Issue: 4; Journal ID: ISSN 1867-2450
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; asymmetric bilayers; lipid domains; lipid flip-flog; lipid-protein interactions; intermembrane interactions; small-angle neutron and x-ray scattering

Citation Formats

Heberle, Frederick A., and Pabst, Georg. Complex biomembrane mimetics on the sub-nanometer scale. United States: N. p., 2017. Web. doi:10.1007/s12551-017-0275-5.
Heberle, Frederick A., & Pabst, Georg. Complex biomembrane mimetics on the sub-nanometer scale. United States. doi:10.1007/s12551-017-0275-5.
Heberle, Frederick A., and Pabst, Georg. Mon . "Complex biomembrane mimetics on the sub-nanometer scale". United States. doi:10.1007/s12551-017-0275-5.
@article{osti_1371488,
title = {Complex biomembrane mimetics on the sub-nanometer scale},
author = {Heberle, Frederick A. and Pabst, Georg},
abstractNote = {Biomimetic lipid vesicles are indispensable tools for gaining insight into the biophysics of cell physiology on the molecular level. The level of complexity of these model systems has steadily increased, and now spans from domain forming lipid mixtures to asymmetric lipid bilayers. We review recent progress in the development and application of elastic neutron and X-ray scattering techniques for studying these systems in situ and under physiologically relevant conditions on the nanometer to sub-nanometer length scales. Particularly we focus on: (i) structural details of coexisting liquid-ordered and liquid-disordered domains, including their thickness and lipid packing mismatch as a function of a size transition from nanoscopic to macroscopic domains; (ii) membrane-mediated protein partitioning into lipid domains; (iii) the role of the aqueous medium in tuning interactions between membranes and domains; and (iv) leaflet specific structure in asymmetric bilayers and passive lipid flip-flop.},
doi = {10.1007/s12551-017-0275-5},
journal = {Biophysical Reviews},
number = 4,
volume = 9,
place = {United States},
year = {2017},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1007/s12551-017-0275-5

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Works referenced in this record:

Lateral sorting in model membranes by cholesterol-mediated hydrophobic matching
journal, September 2011

  • Kaiser, H. -J.; Orlowski, A.; Rog, T.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 40
  • DOI: 10.1073/pnas.1103742108

Closed-Loop Miscibility Gap and Quantitative Tie-Lines in Ternary Membranes Containing Diphytanoyl PC
journal, June 2006


Microdomains in Lipid Vesicles: Structure and Distribution Assessed by Small-Angle Neutron Scattering
journal, April 2010

  • Vogtt, Karsten; Jeworrek, Christoph; Garamus, Vasil M.
  • The Journal of Physical Chemistry B, Vol. 114, Issue 16
  • DOI: 10.1021/jp101167n

Direct chemical evidence for sphingolipid domains in the plasma membranes of fibroblasts
journal, January 2013

  • Frisz, J. F.; Lou, K.; Klitzing, H. A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 8
  • DOI: 10.1073/pnas.1216585110

Energetics of Hydrophobic Matching in Lipid-Protein Interactions
journal, May 2008


Ternary Lipid Bilayers Containing Cholesterol in a High Curvature Silica Xerogel Environment
journal, June 2010

  • Goksu, Emel I.; Longo, Marjorie L.
  • Langmuir, Vol. 26, Issue 11
  • DOI: 10.1021/la9046885

Three-dimensional segregation of supramolecular activation clusters in T cells
journal, September 1998

  • Monks, Colin R. F.; Freiberg, Benjamin A.; Kupfer, Hannah
  • Nature, Vol. 395, Issue 6697
  • DOI: 10.1038/25764

Ternary Phase Diagram of Dipalmitoyl-PC/Dilauroyl-PC/Cholesterol: Nanoscopic Domain Formation Driven by Cholesterol
journal, June 2001


Engineering asymmetric vesicles
journal, September 2003

  • Pautot, S.; Frisken, B. J.; Weitz, D. A.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 19
  • DOI: 10.1073/pnas.1931005100

‘Zur Lehre von der Wirkung der Salze’ (about the science of the effect of salts): Franz Hofmeister's historical papers
journal, August 2004

  • Kunz, W.; Henle, J.; Ninham, B. W.
  • Current Opinion in Colloid & Interface Science, Vol. 9, Issue 1-2
  • DOI: 10.1016/j.cocis.2004.05.005

Comparison of Three Ternary Lipid Bilayer Mixtures: FRET and ESR Reveal Nanodomains
journal, November 2010


A new method for the evaluation of small-angle scattering data
journal, October 1977


Structure of lipid bilayers
journal, November 2000

  • Nagle, John F.; Tristram-Nagle, Stephanie
  • Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, Vol. 1469, Issue 3
  • DOI: 10.1016/S0304-4157(00)00016-2

Theory of the structure factor of lipid bilayers
journal, December 1994


A detailed analysis of partial molecular volumes in DPPC/cholesterol binary bilayers
journal, December 2014

  • Miyoshi, Tsubasa; Lönnfors, Max; Peter Slotte, J.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1838, Issue 12
  • DOI: 10.1016/j.bbamem.2014.07.004

Synthetic peptides as models for intrinsic membrane proteins
journal, October 2003


Small angle X-ray scattering as a high-throughput method to classify antimicrobial modes of action
journal, May 2016

  • von Gundlach, A. R.; Garamus, V. M.; Gorniak, T.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 5
  • DOI: 10.1016/j.bbamem.2015.12.022

Partition profile of the nicotinic acetylcholine receptor in lipid domains upon reconstitution
journal, June 2010

  • Bermúdez, Vicente; Antollini, Silvia S.; Nievas, Gaspar A. Fernández
  • Journal of Lipid Research, Vol. 51, Issue 9
  • DOI: 10.1194/jlr.M005132

Phase diagram of a polyunsaturated lipid mixture: Brain sphingomyelin/1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine/cholesterol
journal, January 2016

  • Konyakhina, Tatyana M.; Feigenson, Gerald W.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 1
  • DOI: 10.1016/j.bbamem.2015.10.016

Scattering from laterally heterogeneous vesicles. I. Model-independent analysis
journal, November 2006

  • Pencer, Jeremy; Anghel, Vinicius N. P.; Kučerka, Norbert
  • Journal of Applied Crystallography, Vol. 39, Issue 6
  • DOI: 10.1107/S0021889806035163

Phase Separation in Biological Membranes: Integration of Theory and Experiment
journal, April 2010


Location of Cholesterol in DMPC Membranes. A Comparative Study by Neutron Diffraction and Molecular Mechanics Simulation
journal, March 2001

  • Léonard, Alain; Escrive, Céline; Laguerre, Michel
  • Langmuir, Vol. 17, Issue 6
  • DOI: 10.1021/la001382p

Control of a Nanoscopic-to-Macroscopic Transition: Modulated Phases in Four-Component DSPC/DOPC/POPC/Chol Giant Unilamellar Vesicles
journal, July 2011

  • Konyakhina, Tatyana M.; Goh, Shih Lin; Amazon, Jonathan
  • Biophysical Journal, Vol. 101, Issue 2
  • DOI: 10.1016/j.bpj.2011.06.019

Relationships between plasma membrane microdomains and HIV-1 assembly
journal, June 2010


Global small-angle X-ray scattering data analysis for multilamellar vesicles: the evolution of the scattering density profile model
journal, December 2013

  • Heftberger, Peter; Kollmitzer, Benjamin; Heberle, Frederick A.
  • Journal of Applied Crystallography, Vol. 47, Issue 1
  • DOI: 10.1107/S1600576713029798

Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles
journal, February 2017

  • Eicher, Barbara; Heberle, Frederick A.; Marquardt, Drew
  • Journal of Applied Crystallography, Vol. 50, Issue 2
  • DOI: 10.1107/S1600576717000656

The atomic force microscope as a tool for studying phase separation in lipid membranes (Review)
journal, January 2006


Measuring shape fluctuations in biological membranes
journal, May 2016


Lateral pressure-mediated protein partitioning into liquid-ordered/liquid-disordered domains
journal, January 2016

  • Frewein, Moritz; Kollmitzer, Benjamin; Heftberger, Peter
  • Soft Matter, Vol. 12, Issue 13
  • DOI: 10.1039/C6SM00042H

Critical Particle Sizes for the Engulfment of Nanoparticles by Membranes and Vesicles with Bilayer Asymmetry
journal, February 2015


Plasma membrane organization and function: moving past lipid rafts
journal, September 2013


Lipid Bilayer Structure Determined by the Simultaneous Analysis of Neutron and X-Ray Scattering Data
journal, September 2008


Phosphatidylserine, a death knell
journal, June 2001


Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes
journal, August 2012

  • Shim, S. -H.; Xia, C.; Zhong, G.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 35
  • DOI: 10.1073/pnas.1201882109

Membrane mechanical properties of synthetic asymmetric phospholipid vesicles
journal, January 2016

  • Lu, Li; Doak, William J.; Schertzer, Jeffrey W.
  • Soft Matter, Vol. 12, Issue 36
  • DOI: 10.1039/c6sm01349j

Structural information from multilamellar liposomes at full hydration: Full q -range fitting with high quality x-ray data
journal, September 2000


Are specific nonannular cholesterol binding sites present in G-protein coupled receptors?
journal, February 2009

  • Paila, Yamuna Devi; Tiwari, Shrish; Chattopadhyay, Amitabha
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1788, Issue 2
  • DOI: 10.1016/j.bbamem.2008.11.020

Membrane-protein topology
journal, December 2006

  • von Heijne, Gunnar
  • Nature Reviews Molecular Cell Biology, Vol. 7, Issue 12
  • DOI: 10.1038/nrm2063

The dependence of lipid asymmetry upon phosphatidylcholine acyl chain structure
journal, October 2012

  • Son, Mijin; London, Erwin
  • Journal of Lipid Research, Vol. 54, Issue 1
  • DOI: 10.1194/jlr.M032722

Partial molecular volumes of lipids and cholesterol
journal, September 2006


Using small-angle neutron scattering to detect nanoscopic lipid domains
journal, May 2013


Structure, Composition, and Peptide Binding Properties of Detergent Soluble Bilayers and Detergent Resistant Rafts
journal, March 2002


Preparation and Properties of Asymmetric Vesicles That Mimic Cell Membranes
journal, January 2009

  • Cheng, Hui-Ting; London, Erwin
  • Journal of Biological Chemistry, Vol. 284, Issue 10
  • DOI: 10.1074/jbc.M806077200

Lipid sorting in epithelial cells
journal, August 1988


Lipid bilayers: clusters, domains and phases
journal, February 2015

  • Ackerman, David G.; Feigenson, Gerald W.
  • Essays in Biochemistry, Vol. 57
  • DOI: 10.1042/BSE0570033

Phase studies of model biomembranes: Complex behavior of DSPC/DOPC/Cholesterol
journal, November 2007

  • Zhao, Jiang; Wu, Jing; Heberle, Frederick A.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1768, Issue 11
  • DOI: 10.1016/j.bbamem.2007.07.008

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
journal, February 2008


Fluid phase lipid areas and bilayer thicknesses of commonly used phosphatidylcholines as a function of temperature
journal, November 2011

  • Kučerka, Norbert; Nieh, Mu-Ping; Katsaras, John
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1808, Issue 11
  • DOI: 10.1016/j.bbamem.2011.07.022

Lipid rafts: contentious only from simplistic standpoints
journal, April 2006

  • Hancock, John F.
  • Nature Reviews Molecular Cell Biology, Vol. 7, Issue 6
  • DOI: 10.1038/nrm1925

1,2-Diacyl-Phosphatidylcholine Flip-Flop Measured Directly by Sum-Frequency Vibrational Spectroscopy
journal, October 2005


Molecular Structures of Fluid Phosphatidylethanolamine Bilayers Obtained from Simulation-to-Experiment Comparisons and Experimental Scattering Density Profiles
journal, December 2014

  • Kučerka, Norbert; van Oosten, Brad; Pan, Jianjun
  • The Journal of Physical Chemistry B, Vol. 119, Issue 5
  • DOI: 10.1021/jp511159q

Triton Promotes Domain Formation in Lipid Raft Mixtures
journal, November 2002


Regulation of transbilayer plasma membrane phospholipid asymmetry
journal, December 2002


Alamethicin in lipid bilayers: Combined use of X-ray scattering and MD simulations
journal, June 2009

  • Pan, Jianjun; Tieleman, D. Peter; Nagle, John F.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1788, Issue 6
  • DOI: 10.1016/j.bbamem.2009.02.013

Small-scale composition fluctuations and microdomain formation in lipid raft models as revealed by small-angle neutron scattering
journal, January 2004

  • Nicolini, Chiara; Thiyagarajan, Pappannan; Winter, Roland
  • Physical Chemistry Chemical Physics, Vol. 6, Issue 24
  • DOI: 10.1039/b408928f

Lipid segregation and IgE receptor signaling: A decade of progress
journal, December 2005

  • Holowka, David; Gosse, Julie A.; Hammond, Adam T.
  • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1746, Issue 3
  • DOI: 10.1016/j.bbamcr.2005.06.007

Cholesterol-induced fluid membrane domains: A compendium of lipid-raft ternary phase diagrams
journal, October 2009


Detection of submicron-sized raft-like domains in membranes by small-angle neutron scattering
journal, November 2005


Applications of neutron and X-ray scattering to the study of biologically relevant model membranes
journal, June 2010


Sphingomyelin/Phosphatidylcholine/Cholesterol Phase Diagram: Boundaries and Composition of Lipid Rafts
journal, October 2003


Co-existence of gel and fluid lipid domains in single-component phospholipid membranes
journal, January 2012

  • Armstrong, C. L.; Barrett, M. A.; Toppozini, L.
  • Soft Matter, Vol. 8, Issue 17
  • DOI: 10.1039/c2sm07158d

Effect of cholesterol on structural and mechanical properties of membranes depends on lipid chain saturation
journal, August 2009


Flip-Flop of Phospholipids in Vesicles: Kinetic Analysis with Time-Resolved Small-Angle Neutron Scattering
journal, May 2009

  • Nakano, Minoru; Fukuda, Masakazu; Kudo, Takayuki
  • The Journal of Physical Chemistry B, Vol. 113, Issue 19
  • DOI: 10.1021/jp900913w

Measuring Lipid Asymmetry in Planar Supported Bilayers by Fluorescence Interference Contrast Microscopy
journal, February 2005

  • Crane, Jonathan M.; Kiessling, Volker; Tamm, Lukas K.
  • Langmuir, Vol. 21, Issue 4
  • DOI: 10.1021/la047654w

Functional rafts in cell membranes
journal, June 1997

  • Simons, Kai; Ikonen, Elina
  • Nature, Vol. 387, Issue 6633
  • DOI: 10.1038/42408

Domain Shapes and Patterns: The Phenomenology of Modulated Phases
journal, January 1995


Atomically detailed lipid bilayer models for the interpretation of small angle neutron and X-ray scattering data
journal, February 2015

  • Fogarty, Joseph C.; Arjunwadkar, Mihir; Pandit, Sagar A.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1848, Issue 2
  • DOI: 10.1016/j.bbamem.2014.10.041

GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane
journal, April 2015

  • Sevcsik, Eva; Brameshuber, Mario; Fölser, Martin
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms7969

Interpretation of Solution X-Ray Scattering by Explicit-Solvent Molecular Dynamics
journal, May 2015


On scattered waves and lipid domains: detecting membrane rafts with X-rays and neutrons
journal, January 2015

  • Marquardt, Drew; Heberle, Frederick A.; Nickels, Jonathan D.
  • Soft Matter, Vol. 11, Issue 47
  • DOI: 10.1039/C5SM01807B

Subnanometer Structure of an Asymmetric Model Membrane: Interleaflet Coupling Influences Domain Properties
journal, May 2016


Lipid Composition and the Lateral Pressure Profile in Bilayers
journal, May 1999


Tuning lipid mixtures to induce or suppress domain formation across leaflets of unsupported asymmetric bilayers
journal, January 2008

  • Collins, M. D.; Keller, S. L.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 1
  • DOI: 10.1073/pnas.0702970105

Transbilayer asymmetry and sphingomyelin composition modulate the preferential membrane partitioning of the nicotinic acetylcholine receptor in Lo domains
journal, February 2016

  • Perillo, Vanesa L.; Peñalva, Daniel A.; Vitale, Alejandro J.
  • Archives of Biochemistry and Biophysics, Vol. 591
  • DOI: 10.1016/j.abb.2015.12.003

Effect of Lipid Characteristics on the Structure of Transmembrane Proteins
journal, September 1998


Monolayer spontaneous curvature of raft-forming membrane lipids
journal, January 2013

  • Kollmitzer, Benjamin; Heftberger, Peter; Rappolt, Michael
  • Soft Matter, Vol. 9, Issue 45
  • DOI: 10.1039/c3sm51829a

Elliptical Structure of Phospholipid Bilayer Nanodiscs Encapsulated by Scaffold Proteins: Casting the Roles of the Lipids and the Protein
journal, October 2010

  • Skar-Gislinge, Nicholas; Simonsen, Jens Bæk; Mortensen, Kell
  • Journal of the American Chemical Society, Vol. 132, Issue 39
  • DOI: 10.1021/ja1030613

Phase behavior and domain size in sphingomyelin-containing lipid bilayers
journal, April 2013

  • Petruzielo, Robin S.; Heberle, Frederick A.; Drazba, Paul
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1828, Issue 4
  • DOI: 10.1016/j.bbamem.2013.01.007

Model-based approaches for the determination of lipid bilayer structure from small-angle neutron and X-ray scattering data
journal, May 2012

  • Heberle, Frederick A.; Pan, Jianjun; Standaert, Robert F.
  • European Biophysics Journal, Vol. 41, Issue 10
  • DOI: 10.1007/s00249-012-0817-5

Neutron diffraction studies on phosphatidylcholine model membranes
journal, November 1979


Thermodynamics and Mechanics of Membrane Curvature Generation and Sensing by Proteins and Lipids
journal, May 2011


Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes
journal, July 2009

  • Lundbæk, Jens A.; Collingwood, Shemille A.; Ingólfsson, Helgi I.
  • Journal of The Royal Society Interface, Vol. 7, Issue 44
  • DOI: 10.1098/rsif.2009.0443

Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
journal, September 2006


Neutron diffraction studies on selectively deuterated phospholipid bilayers
journal, January 1978

  • BÜLdt, G.; Gally, H. U.; Seelig, A.
  • Nature, Vol. 271, Issue 5641
  • DOI: 10.1038/271182a0

Revisiting the bilayer structures of fluid phase phosphatidylglycerol lipids: Accounting for exchangeable hydrogens
journal, November 2014

  • Pan, Jianjun; Marquardt, Drew; Heberle, Frederick A.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1838, Issue 11
  • DOI: 10.1016/j.bbamem.2014.08.009

Sensing isothermal changes in the lateral pressure in model membranes using di-pyrenyl phosphatidylcholine
journal, January 1999

  • Templer, Richard H.; Castle, Saffron J.; Rachael Curran, A.
  • Faraday Discussions, Vol. 111
  • DOI: 10.1039/a806472e

Lattice simulations of phase morphology on lipid bilayers: Renormalization, membrane shape, and electrostatic dipole interactions
journal, February 2014


Bending Rigidities and Interdomain Forces in Membranes with Coexisting Lipid Domains
journal, June 2015

  • Kollmitzer, Benjamin; Heftberger, Peter; Podgornik, Rudolf
  • Biophysical Journal, Vol. 108, Issue 12
  • DOI: 10.1016/j.bpj.2015.05.003

How lipids affect the activities of integral membrane proteins
journal, November 2004


Scattering from phase-separated vesicles. I. An analytical form factor for multiple static domains
journal, August 2015

  • Heberle, Frederick A.; Anghel, Vinicius N. P.; Katsaras, John
  • Journal of Applied Crystallography, Vol. 48, Issue 5
  • DOI: 10.1107/S160057671501362X

The study of biological structures by neutron scattering from solution
journal, October 1976


High-resolution structure of coexisting nanoscopic and microscopic lipid domains
journal, January 2017

  • Belička, Michal; Weitzer, Anna; Pabst, Georg
  • Soft Matter, Vol. 13, Issue 9
  • DOI: 10.1039/c6sm02727j

Modulation of Elasticity and Interactions in Charged Lipid Multibilayers: Monovalent Salt Solutions
journal, December 2016


Supported Lipid Bilayers on Mica and Silicon Oxide: Comparison of the Main Phase Transition Behavior
journal, July 2010

  • Seeger, Heiko M.; Cerbo, Alessandro Di; Alessandrini, Andrea
  • The Journal of Physical Chemistry B, Vol. 114, Issue 27
  • DOI: 10.1021/jp1026477

Raft Partitioning and Dynamic Behavior of Human Placental Alkaline Phosphatase in Giant Unilamellar Vesicles
journal, May 2005

  • Kahya, Nicoletta; Brown, Deborah A.; Schwille, Petra
  • Biochemistry, Vol. 44, Issue 20
  • DOI: 10.1021/bi047429d

Competition between line tension and curvature stabilizes modulated phase patterns on the surface of giant unilamellar vesicles: A simulation study
journal, February 2013


Simulation-Based Methods for Interpreting X-Ray Data from Lipid Bilayers
journal, April 2006


Bilayer Thickness Mismatch Controls Domain Size in Model Membranes
journal, February 2013

  • Heberle, Frederick A.; Petruzielo, Robin S.; Pan, Jianjun
  • Journal of the American Chemical Society, Vol. 135, Issue 18
  • DOI: 10.1021/ja3113615

Dynamic Molecular Structure of DPPC-DLPC-Cholesterol Ternary Lipid System by Spin-Label Electron Spin Resonance
journal, October 2004


Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers
journal, April 2017

  • Usery, Rebecca D.; Enoki, Thais A.; Wickramasinghe, Sanjula P.
  • Biophysical Journal, Vol. 112, Issue 7
  • DOI: 10.1016/j.bpj.2017.02.033

Comparison of Actin- and Glass-Supported Phospholipid Bilayer Diffusion Coefficients
journal, April 2015

  • Sterling, Sarah M.; Dawes, Ryan; Allgeyer, Edward S.
  • Biophysical Journal, Vol. 108, Issue 8
  • DOI: 10.1016/j.bpj.2015.02.033

Lipid Rafts Reconstituted in Model Membranes
journal, March 2001


The molecular structure of a phosphatidylserine bilayer determined by scattering and molecular dynamics simulations
journal, January 2014

  • Pan, Jianjun; Cheng, Xiaolin; Monticelli, Luca
  • Soft Matter, Vol. 10, Issue 21
  • DOI: 10.1039/c4sm00066h

Determination of Tie-Line Fields for Coexisting Lipid Phases: An ESR Study
journal, March 2009

  • Smith, Andrew K.; Freed, Jack H.
  • The Journal of Physical Chemistry B, Vol. 113, Issue 12
  • DOI: 10.1021/jp808412x

Lipid Domain Co-localization Induced by Membrane Undulations
journal, February 2017


Measurement of forces between lecithin bilayers
journal, February 1976

  • Leneveu, D. M.; Rand, R. P.; Parsegian, V. A.
  • Nature, Vol. 259, Issue 5544
  • DOI: 10.1038/259601a0

Phase transition behaviors of the supported DPPC bilayer investigated by sum frequency generation (SFG) vibrational spectroscopy and atomic force microscopy (AFM)
journal, January 2016

  • Wu, Heng-Liang; Tong, Yujin; Peng, Qiling
  • Physical Chemistry Chemical Physics, Vol. 18, Issue 3
  • DOI: 10.1039/c5cp04960a

Condensed complexes of cholesterol and phospholipids
journal, March 2003

  • McConnell, Harden M.; Radhakrishnan, Arun
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1610, Issue 2
  • DOI: 10.1016/S0005-2736(03)00015-4

Neutron Diffraction Studies on the Location of Water in Lecithin Bilayer Model Membranes
journal, January 1975

  • Zaccai, G.; Blasie, J. K.; Schoenborn, B. P.
  • Proceedings of the National Academy of Sciences, Vol. 72, Issue 1
  • DOI: 10.1073/pnas.72.1.376

Transmembrane Asymmetry and Lateral Domains in Biological Membranes: Rafts and Transmembrane Asymmetry
journal, March 2004


Phase Separation in Lipid Membranes
journal, March 2011


Seeing spots: Complex phase behavior in simple membranes
journal, December 2005

  • Veatch, Sarah L.; Keller, Sarah L.
  • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1746, Issue 3
  • DOI: 10.1016/j.bbamcr.2005.06.010

Flicker Spectroscopy of Thermal Lipid Bilayer Domain Boundary Fluctuations
journal, November 2007


Asymmetrical Lipid Bilayer Structure for Biological Membranes
journal, March 1972


Nanodiscs for structural and functional studies of membrane proteins
journal, June 2016

  • Denisov, Ilia G.; Sligar, Stephen G.
  • Nature Structural & Molecular Biology, Vol. 23, Issue 6
  • DOI: 10.1038/nsmb.3195

Free Energy and Entropy of Activation for Phospholipid Flip-Flop in Planar Supported Lipid Bilayers
journal, February 2010

  • Anglin, Timothy C.; Cooper, Michael P.; Li, Hao
  • The Journal of Physical Chemistry B, Vol. 114, Issue 5
  • DOI: 10.1021/jp909134g

The Immunological Synapse: A Molecular Machine Controlling T Cell Activation
journal, July 1999


Nano-meter-sized domain formation in lipid membranes observed by small angle neutron scattering
journal, December 2008


Protein modulation of lipids, and vice-versa, in membranes
journal, July 2008


Diffusion in supported lipid bilayers: Influence of substrate and preparation technique on the internal dynamics
journal, December 2008


Lipid–protein interactions in biological membranes: A dynamic perspective
journal, February 2012


Long-range interlayer alignment of intralayer domains in stacked lipid bilayers
journal, October 2012

  • Tayebi, Lobat; Ma, Yicong; Vashaee, Daryoosh
  • Nature Materials, Vol. 11, Issue 12
  • DOI: 10.1038/NMAT3451

Scattering from laterally heterogeneous vesicles. II. The form factor
journal, May 2007

  • Anghel, Vinicius N. P.; Kučerka, Norbert; Pencer, Jeremy
  • Journal of Applied Crystallography, Vol. 40, Issue 3
  • DOI: 10.1107/S002188980701206X

The organization of n-alkanes in lipid bilayers
journal, April 1980

  • McIntosh, T. J.; Simon, S. A.; MacDonald, R. C.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 597, Issue 3
  • DOI: 10.1016/0005-2736(80)90219-9

Lateral Pressures in Cell Membranes:  A Mechanism for Modulation of Protein Function
journal, March 1997

  • Cantor, Robert S.
  • The Journal of Physical Chemistry B, Vol. 101, Issue 10
  • DOI: 10.1021/jp963911x

Lipid rafts and membrane dynamics
journal, February 2005


Small-angle neutron scattering study of lipid phase diagrams by the contrast variation method
journal, October 1981

  • Knoll, Wolfgang; Ibel, Konrad; Sackmann, Erich
  • Biochemistry, Vol. 20, Issue 22
  • DOI: 10.1021/bi00525a015

Short-range interactions between lipid bilayers measured by X-ray diffraction
journal, August 2000


Use of Cyclodextrins to Monitor Transbilayer Movement and Differential Lipid Affinities of Cholesterol
journal, October 2001


Membrane-induced interactions between inclusions
journal, November 1993


Specific volumes of unsaturated phosphatidylcholines in the liquid crystalline lamellar phase
journal, August 2005

  • Koenig, Bernd W.; Gawrisch, Klaus
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1715, Issue 1
  • DOI: 10.1016/j.bbamem.2005.07.006

Direct observation of the nanoscale dynamics of membrane lipids in a living cell
journal, December 2008

  • Eggeling, Christian; Ringemann, Christian; Medda, Rebecca
  • Nature, Vol. 457, Issue 7233
  • DOI: 10.1038/nature07596

1 H NMR Shows Slow Phospholipid Flip-Flop in Gel and Fluid Bilayers
journal, February 2017


Critical fluctuations in domain-forming lipid mixtures
journal, October 2007

  • Veatch, S. L.; Soubias, O.; Keller, S. L.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 45
  • DOI: 10.1073/pnas.0703513104

SNAREs Prefer Liquid-disordered over “Raft” (Liquid-ordered) Domains When Reconstituted into Giant Unilamellar Vesicles
journal, June 2004

  • Bacia, Kirsten; Schuette, Christina G.; Kahya, Nicoletta
  • Journal of Biological Chemistry, Vol. 279, Issue 36
  • DOI: 10.1074/jbc.M407020200

Interactions between Ether Phospholipids and Cholesterol As Determined by Scattering and Molecular Dynamics Simulations
journal, December 2012

  • Pan, Jianjun; Cheng, Xiaolin; Heberle, Frederick A.
  • The Journal of Physical Chemistry B, Vol. 116, Issue 51
  • DOI: 10.1021/jp310345j

Component volumes of unsaturated phosphatidylcholines in fluid bilayers: a densitometric study
journal, February 2007


In Situ Determination of Structure and Fluctuations of Coexisting Fluid Membrane Domains
journal, February 2015

  • Heftberger, Peter; Kollmitzer, Benjamin; Rieder, Alexander A.
  • Biophysical Journal, Vol. 108, Issue 4
  • DOI: 10.1016/j.bpj.2014.11.3488

Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol
journal, September 2013

  • Konyakhina, Tatyana M.; Wu, Jing; Mastroianni, James D.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1828, Issue 9
  • DOI: 10.1016/j.bbamem.2013.05.020

Molecular and component volumes of saturated n-alkanols in DOPC+DOPS bilayers
journal, June 2010


Phase Diagram of Ternary Cholesterol/Palmitoylsphingomyelin/Palmitoyloleoyl-Phosphatidylcholine Mixtures: Spin-Label EPR Study of Lipid-Raft Formation
journal, April 2012

  • Ionova, Irina V.; Livshits, Vsevolod A.; Marsh, Derek
  • Biophysical Journal, Vol. 102, Issue 8
  • DOI: 10.1016/j.bpj.2012.03.043

Lipid Rafts, Detergent-Resistant Membranes, and Raft Targeting Signals
journal, December 2006


Structure of Detergent-Resistant Membrane Domains: Does Phase Separation Occur in Biological Membranes?
journal, November 1997

  • Brown, Deborah A.; London, Erwin
  • Biochemical and Biophysical Research Communications, Vol. 240, Issue 1
  • DOI: 10.1006/bbrc.1997.7575

Hybrid and Nonhybrid Lipids Exert Common Effects on Membrane Raft Size and Morphology
journal, September 2013

  • Heberle, Frederick A.; Doktorova, Milka; Goh, Shih Lin
  • Journal of the American Chemical Society, Vol. 135, Issue 40
  • DOI: 10.1021/ja407624c

Phase diagrams and lipid domains in multicomponent lipid bilayer mixtures
journal, January 2009


Structure and polymorphism of the hydrocarbon chains of lipids: A study of lecithin-water phases
journal, April 1973