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This content will become publicly available on March 5, 2018

Title: Kaposi’s sarcoma–associated herpesvirus stably clusters its genomes across generations to maintain itself extrachromosomally

Genetic elements that replicate extrachromosomally are rare in mammals; however, several human tumor viruses, including the papillomaviruses and the gammaherpesviruses, maintain their plasmid genomes by tethering them to cellular chromosomes. We have uncovered an unprecedented mechanism of viral replication: Kaposi’s sarcoma–associated herpesvirus (KSHV) stably clusters its genomes across generations to maintain itself extrachromosomally. To identify and characterize this mechanism, we developed two complementary, independent approaches: live-cell imaging and a predictive computational model. The clustering of KSHV requires the viral protein, LANA1, to bind viral genomes to nucleosomes arrayed on both cellular and viral DNA. Clustering affects both viral partitioning and viral genome numbers of KSHV. The clustering of KSHV plasmids provides it with an effective evolutionary strategy to rapidly increase copy numbers of genomes per cell at the expense of the total numbers of cells infected.
Authors:
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Publication Date:
Type:
Published Article
Journal Name:
Journal of Cell Biology
Additional Journal Information:
Related Information: CHORUS Timestamp: 2017-07-10 11:05:29; Journal ID: ISSN 0021-9525
Publisher:
Rockefeller University Press
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
OSTI Identifier:
1368608