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Title: Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses

Abstract

This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. AerosolizedOmono River virusparticles of ~40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ~35 to ~300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012photons per µm2per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under thesemore » conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. Finally, the results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.« less

Authors:
ORCiD logo [1];  [1];  [1];  [2];  [1];  [1];  [1]; ORCiD logo [1];  [3];  [1]; ORCiD logo [1];  [4];  [5];  [1];  [1];  [6];  [1];  [7];  [8];  [4] more »;  [1];  [1];  [1];  [9];  [4];  [10];  [11];  [12];  [11]; ORCiD logo [1];  [13];  [1] « less
  1. Uppsala Univ., Uppsala (Sweden)
  2. Uppsala Univ., Uppsala (Sweden); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. National Univ. of Singapore (Singapore)
  6. Uppsala Univ., Uppsala (Sweden); Bhabha Atomic Research Center, Mumbai (India)
  7. Arizona State Univ., Tempe, AZ (United States); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  9. Uppsala Univ., Uppsala (Sweden); KTH Royal Institute of Technology, Stockholm (Sweden)
  10. Uppsala Univ., Uppsala (Sweden); Czech Academy of Science, Prague (Czech Republic)
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  12. Brookhaven National Lab. (BNL), Upton, NY (United States)
  13. Uppsala Univ., Uppsala (Sweden); Institute of Physics AS CR, Prague (Czech Republic)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1368570
Alternate Identifier(s):
OSTI ID: 1376140; OSTI ID: 1413724; OSTI ID: 1525396
Report Number(s):
BNL-114080-2017-JA; BNL-114080-2017-JAAM
Journal ID: ISSN 2052-2525; IUCRAJ; PII: S2052252517003591
Grant/Contract Number:  
AC02-76SF00515; SC00112704; AC02-05CH11231; SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 4; Journal Issue: 3; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; X-ray diffraction; free-electron laser; flash X-ray imaging; diffraction before destruction; virus; Omono River virus; OmRV; 59 BASIC BIOLOGICAL SCIENCES; omono river virus

Citation Formats

Daurer, Benedikt J., Okamoto, Kenta, Bielecki, Johan, Maia, Filipe R. N. C., Muhlig, Kerstin, Seibert, M. Marvin, Hantke, Max F., Nettelblad, Carl, Benner, W. Henry, Svenda, Martin, Timneanu, Nicusor, Ekeberg, Tomas, Loh, N. Duane, Pietrini, Alberto, Zani, Alessandro, Rath, Asawari D., Westphal, Daniel, Kirian, Richard A., Awel, Salah, Wiedorn, Max O., van der Schot, Gijs, Carlsson, Gunilla H., Hasse, Dirk, Sellberg, Jonas A., Barty, Anton, Andreasson, Jakob, Boutet, Sebastien, Williams, Garth, Koglin, Jason, Andersson, Inger, Hajdu, Janos, and Larsson, Daniel S. D. Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. United States: N. p., 2017. Web. doi:10.1107/S2052252517003591.
Daurer, Benedikt J., Okamoto, Kenta, Bielecki, Johan, Maia, Filipe R. N. C., Muhlig, Kerstin, Seibert, M. Marvin, Hantke, Max F., Nettelblad, Carl, Benner, W. Henry, Svenda, Martin, Timneanu, Nicusor, Ekeberg, Tomas, Loh, N. Duane, Pietrini, Alberto, Zani, Alessandro, Rath, Asawari D., Westphal, Daniel, Kirian, Richard A., Awel, Salah, Wiedorn, Max O., van der Schot, Gijs, Carlsson, Gunilla H., Hasse, Dirk, Sellberg, Jonas A., Barty, Anton, Andreasson, Jakob, Boutet, Sebastien, Williams, Garth, Koglin, Jason, Andersson, Inger, Hajdu, Janos, & Larsson, Daniel S. D. Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. United States. doi:10.1107/S2052252517003591.
Daurer, Benedikt J., Okamoto, Kenta, Bielecki, Johan, Maia, Filipe R. N. C., Muhlig, Kerstin, Seibert, M. Marvin, Hantke, Max F., Nettelblad, Carl, Benner, W. Henry, Svenda, Martin, Timneanu, Nicusor, Ekeberg, Tomas, Loh, N. Duane, Pietrini, Alberto, Zani, Alessandro, Rath, Asawari D., Westphal, Daniel, Kirian, Richard A., Awel, Salah, Wiedorn, Max O., van der Schot, Gijs, Carlsson, Gunilla H., Hasse, Dirk, Sellberg, Jonas A., Barty, Anton, Andreasson, Jakob, Boutet, Sebastien, Williams, Garth, Koglin, Jason, Andersson, Inger, Hajdu, Janos, and Larsson, Daniel S. D. Fri . "Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses". United States. doi:10.1107/S2052252517003591. https://www.osti.gov/servlets/purl/1368570.
@article{osti_1368570,
title = {Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses},
author = {Daurer, Benedikt J. and Okamoto, Kenta and Bielecki, Johan and Maia, Filipe R. N. C. and Muhlig, Kerstin and Seibert, M. Marvin and Hantke, Max F. and Nettelblad, Carl and Benner, W. Henry and Svenda, Martin and Timneanu, Nicusor and Ekeberg, Tomas and Loh, N. Duane and Pietrini, Alberto and Zani, Alessandro and Rath, Asawari D. and Westphal, Daniel and Kirian, Richard A. and Awel, Salah and Wiedorn, Max O. and van der Schot, Gijs and Carlsson, Gunilla H. and Hasse, Dirk and Sellberg, Jonas A. and Barty, Anton and Andreasson, Jakob and Boutet, Sebastien and Williams, Garth and Koglin, Jason and Andersson, Inger and Hajdu, Janos and Larsson, Daniel S. D.},
abstractNote = {This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. AerosolizedOmono River virusparticles of ~40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ~35 to ~300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012photons per µm2per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. Finally, the results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.},
doi = {10.1107/S2052252517003591},
journal = {IUCrJ},
number = 3,
volume = 4,
place = {United States},
year = {2017},
month = {4}
}

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    Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
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    • Daurer, Benedikt
    • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); University of Gothenburg, University of Jyvaskyla, University of Helsinki, CFEL, The University of Tokyo, Arizona State University, La Trobe University, University of Wisconsin, SLAC National Accelerator Laboratory, RIKEN SPring-8 Center, University of Chicago, Argonne National Laboratory, Northeastern Illinois University
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    Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
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    • Daurer, Benedikt
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