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Title: Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.
Authors:
 [1] ; ORCiD logo [2] ;  [3] ;  [4] ;  [4] ;  [1] ;  [5]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Nuclear Engineering
  2. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Physics and Astronomy
  3. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Nuclear Engineering; Escuela Politecnica Nacional, Quito (Ecuador). Dept. de Ciencias Nucleares
  4. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
  5. Univ. of Chicago, IL (United States)
Publication Date:
Grant/Contract Number:
NA0001983; GM112496
Type:
Published Article
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
Univ. of Tennessee, Knoxville, TN (United States)
Sponsoring Org:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1368434
Alternate Identifier(s):
OSTI ID: 1393145