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Title: A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings

Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. But, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes. We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital negative profiles, to adjust the white balance, and to calibrate the exposure rate differences caused by the environment so that this method is applicable in any environment. We chose an exponential function model to estimate chlorophyll content from the RGB values, and fitted the model parameters with physical measurements of chlorophyll contents. As further proof of utility, this method was used to estimate chlorophyll content of G protein mutants grown on different sugar to nitrogen ratios. Our method is a simple, fast, inexpensive, and nondestructive estimation of chlorophyll content of Arabidopsis seedlings. Thismore » method lead to the discovery that G proteins are important in sensing the C/N balance to control chlorophyll content in Arabidopsis.« less
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [3] ; ORCiD logo [4]
  1. Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Biology; Northwest A&F Univ., Shaanxi (China)
  2. Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Biology
  3. Northwest A&F Univ., Shaanxi (China)
  4. Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Biology and Dept. of Pharmacology
Publication Date:
Grant/Contract Number:
FG02-05ER15671
Type:
Accepted Manuscript
Journal Name:
Plant Methods
Additional Journal Information:
Journal Volume: 13; Journal Issue: 1; Journal ID: ISSN 1746-4811
Publisher:
BioMed Central
Research Org:
Univ. of North Carolina, Chapel Hill, NC (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Arabidopsis thaliana; C/N sensing; Chlorophyll content; ColorChecker chart; Heterotrimeric G protein complex; Stress assay
OSTI Identifier:
1368388

Liang, Ying, Urano, Daisuke, Liao, Kang-Ling, Hedrick, Tyson L., Gao, Yajun, and Jones, Alan M.. A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings. United States: N. p., Web. doi:10.1186/s13007-017-0174-6.
Liang, Ying, Urano, Daisuke, Liao, Kang-Ling, Hedrick, Tyson L., Gao, Yajun, & Jones, Alan M.. A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings. United States. doi:10.1186/s13007-017-0174-6.
Liang, Ying, Urano, Daisuke, Liao, Kang-Ling, Hedrick, Tyson L., Gao, Yajun, and Jones, Alan M.. 2017. "A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings". United States. doi:10.1186/s13007-017-0174-6. https://www.osti.gov/servlets/purl/1368388.
@article{osti_1368388,
title = {A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings},
author = {Liang, Ying and Urano, Daisuke and Liao, Kang-Ling and Hedrick, Tyson L. and Gao, Yajun and Jones, Alan M.},
abstractNote = {Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. But, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes. We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital negative profiles, to adjust the white balance, and to calibrate the exposure rate differences caused by the environment so that this method is applicable in any environment. We chose an exponential function model to estimate chlorophyll content from the RGB values, and fitted the model parameters with physical measurements of chlorophyll contents. As further proof of utility, this method was used to estimate chlorophyll content of G protein mutants grown on different sugar to nitrogen ratios. Our method is a simple, fast, inexpensive, and nondestructive estimation of chlorophyll content of Arabidopsis seedlings. This method lead to the discovery that G proteins are important in sensing the C/N balance to control chlorophyll content in Arabidopsis.},
doi = {10.1186/s13007-017-0174-6},
journal = {Plant Methods},
number = 1,
volume = 13,
place = {United States},
year = {2017},
month = {4}
}