Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]
Abstract
Carbon nanotube porins (CNTPs), small segments of carbon nanotubes capable of forming defined pores in lipid membranes, are important future components for bionanoelectronic devices as they could provide a robust analog of biological membrane channels. Furthermore, in order to control the incorporation of these CNT channels into lipid bilayers, it is important to understand the structure of the CNTPs before and after insertion into the lipid bilayer as well as the impact of such insertion on the bilayer structure. Here we employed a noninvasive in situ probe, small-angle X-ray scattering, to study the integration of CNT porins into dioleoylphosphatidylcholine bilayers. These results show that CNTPs in solution are stabilized by a monolayer of lipid molecules wrapped around their outer surface. We also demonstrate that insertion of CNTPs into the lipid bilayer results in decreased bilayer thickness with the magnitude of this effect increasing with the concentration of CNTPs.
- Authors:
-
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1367965
- Report Number(s):
- LLNL-JRNL-679134
Journal ID: ISSN 1530-6984
- Grant/Contract Number:
- AC52-07NA27344; SCW0972; AC02-76SF00515; P41GM103393
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nano Letters
- Additional Journal Information:
- Journal Volume: 16; Journal Issue: 7; Journal ID: ISSN 1530-6984
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; carbon nanotube; Phospholipid; porins; small-angle X-ray scattering (SAXS)
Citation Formats
Tran, Ich C., Tunuguntla, Ramya H., Kim, Kyunghoon, Lee, Jonathan R. I., Willey, Trevor M., Weiss, Thomas M., Noy, Aleksandr, and van Buuren, Tony. Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]. United States: N. p., 2016.
Web. doi:10.1021/acs.nanolett.6b00466.
Tran, Ich C., Tunuguntla, Ramya H., Kim, Kyunghoon, Lee, Jonathan R. I., Willey, Trevor M., Weiss, Thomas M., Noy, Aleksandr, & van Buuren, Tony. Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]. United States. https://doi.org/10.1021/acs.nanolett.6b00466
Tran, Ich C., Tunuguntla, Ramya H., Kim, Kyunghoon, Lee, Jonathan R. I., Willey, Trevor M., Weiss, Thomas M., Noy, Aleksandr, and van Buuren, Tony. Mon .
"Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]". United States. https://doi.org/10.1021/acs.nanolett.6b00466. https://www.osti.gov/servlets/purl/1367965.
@article{osti_1367965,
title = {Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]},
author = {Tran, Ich C. and Tunuguntla, Ramya H. and Kim, Kyunghoon and Lee, Jonathan R. I. and Willey, Trevor M. and Weiss, Thomas M. and Noy, Aleksandr and van Buuren, Tony},
abstractNote = {Carbon nanotube porins (CNTPs), small segments of carbon nanotubes capable of forming defined pores in lipid membranes, are important future components for bionanoelectronic devices as they could provide a robust analog of biological membrane channels. Furthermore, in order to control the incorporation of these CNT channels into lipid bilayers, it is important to understand the structure of the CNTPs before and after insertion into the lipid bilayer as well as the impact of such insertion on the bilayer structure. Here we employed a noninvasive in situ probe, small-angle X-ray scattering, to study the integration of CNT porins into dioleoylphosphatidylcholine bilayers. These results show that CNTPs in solution are stabilized by a monolayer of lipid molecules wrapped around their outer surface. We also demonstrate that insertion of CNTPs into the lipid bilayer results in decreased bilayer thickness with the magnitude of this effect increasing with the concentration of CNTPs.},
doi = {10.1021/acs.nanolett.6b00466},
journal = {Nano Letters},
number = 7,
volume = 16,
place = {United States},
year = {2016},
month = {6}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 12 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
The unique role of nanoparticles in nanomedicine: imaging, drug delivery and therapy
journal, January 2012
- Doane, Tennyson L.; Burda, Clemens
- Chemical Society Reviews, Vol. 41, Issue 7
Carbon nanotubes in biology and medicine: In vitro and in vivo detection, imaging and drug delivery
journal, February 2009
- Liu, Zhuang; Tabakman, Scott; Welsher, Kevin
- Nano Research, Vol. 2, Issue 2
Promises, facts and challenges for carbon nanotubes in imaging and therapeutics
journal, September 2009
- Kostarelos, K.; Bianco, A.; Prato, M.
- Nature Nanotechnology, Vol. 4, Issue 10
Functionalized Carbon Nanotubes in Drug Design and Discovery
journal, January 2008
- Prato, Maurizio; Kostarelos, Kostas; Bianco, Alberto
- Accounts of Chemical Research, Vol. 41, Issue 1
Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type
journal, January 2007
- Kostarelos, Kostas; Lacerda, Lara; Pastorin, Giorgia
- Nature Nanotechnology, Vol. 2, Issue 2
Carbon nanotube applications for tissue engineering
journal, January 2007
- Harrison, Benjamin S.; Atala, Anthony
- Biomaterials, Vol. 28, Issue 2
Carbon nanotubes as nanomedicines: From toxicology to pharmacology☆
journal, December 2006
- Lacerda, L.; Bianco, A.; Prato, M.
- Advanced Drug Delivery Reviews, Vol. 58, Issue 14
Functionalized carbon nanotubes as emerging nanovectors for the delivery of therapeutics
journal, March 2006
- Klumpp, Cédric; Kostarelos, Kostas; Prato, Maurizio
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 3, p. 404-412
Targeted Delivery of Amphotericin B to Cells by Using Functionalized Carbon Nanotubes
journal, October 2005
- Wu, Wei; Wieckowski, Sébastien; Pastorin, Giorgia
- Angewandte Chemie International Edition, Vol. 44, Issue 39
Binding and Condensation of Plasmid DNA onto Functionalized Carbon Nanotubes: Toward the Construction of Nanotube-Based Gene Delivery Vectors
journal, March 2005
- Singh, Ravi; Pantarotto, Davide; McCarthy, David
- Journal of the American Chemical Society, Vol. 127, Issue 12
Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction
journal, August 2005
- Shi Kam, N. W.; O'Connell, M.; Wisdom, J. A.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 33, p. 11600-11605
Applications of carbon nanotubes in drug delivery
journal, December 2005
- Bianco, Alberto; Kostarelos, Kostas; Prato, Maurizio
- Current Opinion in Chemical Biology, Vol. 9, Issue 6
Biomedical applications of functionalised carbon nanotubes
journal, January 2005
- Bianco, Alberto; Kostarelos, Kostas; Partidos, Charalambos D.
- Chemical Communications, Issue 5
Near-infrared optical sensors based on single-walled carbon nanotubes
journal, December 2004
- Barone, Paul W.; Baik, Seunghyun; Heller, Daniel A.
- Nature Materials, Vol. 4, Issue 1
Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery
journal, October 2004
- Pantarotto, Davide; Singh, Ravi; McCarthy, David
- Angewandte Chemie International Edition, Vol. 43, Issue 39
Can Carbon Nanotubes be Considered Useful Tools for Biological Applications?
journal, October 2003
- Bianco, A.; Prato, M.
- Advanced Materials, Vol. 15, Issue 20
Osmotically-Driven Transport in Carbon Nanotube Porins
journal, November 2014
- Kim, Kyunghoon; Geng, Jia; Tunuguntla, Ramya
- Nano Letters, Vol. 14, Issue 12
Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes
journal, October 2014
- Geng, Jia; Kim, Kyunghoon; Zhang, Jianfei
- Nature, Vol. 514, Issue 7524
Carbon Nanomaterials for Biological Imaging and Nanomedicinal Therapy
journal, April 2015
- Hong, Guosong; Diao, Shuo; Antaris, Alexander L.
- Chemical Reviews, Vol. 115, Issue 19
Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging
journal, January 2011
- Al-Jamal, Khuloud T.; Nerl, Hannah; Müller, Karin H.
- Nanoscale, Vol. 3, Issue 6
Direct imaging of single-walled carbon nanotubes in cells
journal, October 2007
- Porter, Alexandra E.; Gass, Mhairi; Muller, Karin
- Nature Nanotechnology, Vol. 2, Issue 11
Nanotube Molecular Transporters: Internalization of Carbon Nanotube−Protein Conjugates into Mammalian Cells
journal, June 2004
- Shi Kam, Nadine Wong; Jessop, Theodore C.; Wender, Paul A.
- Journal of the American Chemical Society, Vol. 126, Issue 22
Single wall carbon nanotubes enter cells by endocytosis and not membrane penetration
journal, January 2011
- Yaron, Peter N.; Holt, Brian D.; Short, Philip A.
- Journal of Nanobiotechnology, Vol. 9, Issue 1
Understanding nature's design for a nanosyringe
journal, March 2004
- Lopez, C. F.; Nielsen, S. O.; Moore, P. B.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 13
Interactions of End-functionalized Nanotubes with Lipid Vesicles: Spontaneous Insertion and Nanotube Self-Organization
journal, October 2011
- Dutt, Meenakshi; J. Nayhouse, Michael; Kuksenok, Olga
- Current Nanoscience, Vol. 7, Issue 5
Forming transmembrane channels using end-functionalized nanotubes
journal, January 2011
- Dutt, Meenakshi; Kuksenok, Olga; Little, Steven R.
- Nanoscale, Vol. 3, Issue 1
Single-Walled Carbon Nanotubes Noncovalently Functionalized with Lipid Modified Polyethylenimine for siRNA Delivery in Vitro and in Vivo
journal, September 2014
- Siu, King S.; Zheng, Xiufen; Liu, Yanling
- Bioconjugate Chemistry, Vol. 25, Issue 10
Dispersing single-walled carbon nanotubes using common phospholipids with a small amount of polyethylene glycol-phospholipid additives
journal, January 2014
- Sato, Yu-uki; Sano, Masahito
- Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 441
Can carbon nanotube–liposome conjugates address the issues associated with carbon nanotubes in drug delivery?
journal, April 2013
- Regev, Oren; Barenholz, Yechezkel; Peretz, Sivan
- Future Medicinal Chemistry, Vol. 5, Issue 5
Hybrid charge transfer complexes based on archaeal glycolipids wrapping single walled carbon nanotubes
journal, January 2013
- Ingrosso, C.; Bianco, G. V.; Corricelli, M.
- Chemical Communications, Vol. 49, Issue 62
Polyglycerol-Derived Amphiphiles for the Solubilization of Single-Walled Carbon Nanotubes in Water: A Structure-Property Study
journal, November 2011
- Popeney, Chris S.; Setaro, Antonio; Mutihac, Radu-Cristian
- ChemPhysChem, Vol. 13, Issue 1
Dynamic Behavior of Carbon Nanotube and Bio-/Artificial Surfactants Complexes in an Aqueous Environment
journal, September 2011
- Obata, Shigeaki; Honda, Kazumasa
- The Journal of Physical Chemistry C, Vol. 115, Issue 40
Individually Suspended Single-Walled Carbon Nanotubes in Various Surfactants
journal, October 2003
- Moore, Valerie C.; Strano, Michael S.; Haroz, Erik H.
- Nano Letters, Vol. 3, Issue 10, p. 1379-1382
Amino acid functionalisation of water soluble carbon nanotubes
journal, November 2002
- Georgakilas, Vasilios; Tagmatarchis, Nikos; Pantarotto, Davide
- Chemical Communications, Issue 24
High Weight Fraction Surfactant Solubilization of Single-Wall Carbon Nanotubes in Water
journal, February 2003
- Islam, M. F.; Rojas, E.; Bergey, D. M.
- Nano Letters, Vol. 3, Issue 2, p. 269-273
Coating Single-Walled Carbon Nanotubes with Phospholipids
journal, February 2006
- Wu, Yonnie; Hudson, JoAn S.; Lu, Qi
- The Journal of Physical Chemistry B, Vol. 110, Issue 6
Small-Angle Neutron Scattering from Surfactant-Assisted Aqueous Dispersions of Carbon Nanotubes
journal, August 2004
- Yurekli, Koray; Mitchell, Cynthia A.; Krishnamoorti, Ramanan
- Journal of the American Chemical Society, Vol. 126, Issue 32, p. 9902-9903
Dispersion of Single-Walled Carbon Nanotubes in Aqueous Solutions of the Anionic Surfactant NaDDBS
journal, December 2003
- Matarredona, Olga; Rhoads, Heather; Li, Zhongrui
- The Journal of Physical Chemistry B, Vol. 107, Issue 48
Insertion of Short Amino-Functionalized Single-Walled Carbon Nanotubes into Phospholipid Bilayer Occurs by Passive Diffusion
journal, July 2012
- Kraszewski, Sebastian; Bianco, Alberto; Tarek, Mounir
- PLoS ONE, Vol. 7, Issue 7
Interparticle Dispersion, Membrane Curvature, and Penetration Induced by Single-Walled Carbon Nanotubes Wrapped with Lipids and PEGylated Lipids
journal, January 2013
- Lee, Hwankyu
- The Journal of Physical Chemistry B, Vol. 117, Issue 5
Embedded Single-Walled Carbon Nanotubes Locally Perturb DOPC Phospholipid Bilayers
journal, October 2012
- Parthasarathi, R.; Tummala, N. R.; Striolo, A.
- The Journal of Physical Chemistry B, Vol. 116, Issue 42
Interaction of Pristine and Functionalized Carbon Nanotubes with Lipid Membranes
journal, September 2013
- Baoukina, Svetlana; Monticelli, Luca; Tieleman, D. Peter
- The Journal of Physical Chemistry B, Vol. 117, Issue 40
Membrane Perturbation by Carbon Nanotube Insertion: Pathways to Internalization
journal, February 2013
- Lelimousin, Mickaël; Sansom, Mark S. P.
- Small, Vol. 9, Issue 21
Spontaneous insertion of carbon nanotube bundles inside biomembranes: A hybrid particle-field coarse-grained molecular dynamics study
journal, March 2014
- Sarukhanyan, Edita; De Nicola, Antonio; Roccatano, Danilo
- Chemical Physics Letters, Vol. 595-596
Introductory Lecture: Basic quantities in model biomembranes
journal, January 2013
- Nagle, John F.
- Faraday Discuss., Vol. 161
Temperature Dependence of Structure, Bending Rigidity, and Bilayer Interactions of Dioleoylphosphatidylcholine Bilayers
journal, January 2008
- Pan, Jianjun; Tristram-Nagle, Stephanie; Kučerka, Norbert
- Biophysical Journal, Vol. 94, Issue 1
The study of liposomes, lamellae and membranes using neutrons and X-rays
journal, February 2007
- Kucerka, N.; Nieh, M.; Pencer, J.
- Current Opinion in Colloid & Interface Science, Vol. 12, Issue 1
Curvature Effect on the Structure of Phospholipid Bilayers
journal, January 2007
- Kučerka, Norbert; Pencer, Jeremy; Sachs, Jonathan N.
- Langmuir, Vol. 23, Issue 3
Effects of cholesterol and unsaturated DOPC lipid on chain packing of saturated gel-phase DPPC bilayers
journal, January 2009
- Mills, T.; Huang, J.; Feigenson, G.
- General Physiology and Biophysics, Vol. 28, Issue 2
Order Parameters and Areas in Fluid-Phase Oriented Lipid Membranes Using Wide Angle X-Ray Scattering
journal, July 2008
- Mills, Thalia T.; Toombes, Gilman E. S.; Tristram-Nagle, Stephanie
- Biophysical Journal, Vol. 95, Issue 2
Liquid-Liquid Domains in Bilayers Detected by Wide Angle X-Ray Scattering
journal, July 2008
- Mills, Thalia T.; Tristram-Nagle, Stephanie; Heberle, Frederick A.
- Biophysical Journal, Vol. 95, Issue 2
Dispersing Single-Walled Carbon Nanotubes with Surfactants: A Small Angle Neutron Scattering Study
journal, September 2004
- Wang, Howard; Zhou, Wei; Ho, Derek L.
- Nano Letters, Vol. 4, Issue 9
Small angle neutron scattering from single-wall carbon nanotube suspensions: evidence for isolated rigid rods and rod networks
journal, January 2004
- Zhou, W.; Islam, M. F.; Wang, H.
- Chemical Physics Letters, Vol. 384, Issue 1-3
Shear-SANS study of single-walled carbon nanotube suspensions
journal, November 2005
- Wang, H.; Christopherson, G. T.; Xu, Z. Y.
- Chemical Physics Letters, Vol. 416, Issue 1-3
Small-Angle Neutron Scattering from Labeled Single-Wall Carbon Nanotubes
journal, April 2006
- Bauer, Barry J.; Hobbie, Erik K.; Becker, Matthew L.
- Macromolecules, Vol. 39, Issue 7
Carbon nanotube self-assembly with lipids and detergent: a molecular dynamics study
journal, December 2008
- Wallace, E. Jayne; Sansom, Mark S. P.
- Nanotechnology, Vol. 20, Issue 4
Irena : tool suite for modeling and analysis of small-angle scattering
journal, February 2009
- Ilavsky, Jan; Jemian, Peter R.
- Journal of Applied Crystallography, Vol. 42, Issue 2
Elucidating the Mechanism of Lipid Membrane-Induced IAPP Fibrillogenesis and Its Inhibition by the Red Wine Compound Resveratrol: A Synchrotron X-ray Reflectivity Study
journal, June 2009
- Evers, Florian; Jeworrek, Christoph; Tiemeyer, Sebastian
- Journal of the American Chemical Society, Vol. 131, Issue 27
Supramolecular Self-Assembly of Lipid Derivatives on Carbon Nanotubes
journal, May 2003
- Richard, C.
- Science, Vol. 300, Issue 5620
Determination of bilayer thickness and lipid surface area in unilamellar dimyristoylphosphatidylcholine vesicles from small-angle neutron scattering curves: a comparison of evaluation methods
journal, September 2003
- Kučerka, Norbert; Kiselev, Mikhail A.; Balgavý, Pavol
- European Biophysics Journal, Vol. 33, Issue 4
Theory of the structure factor of lipid bilayers
journal, December 1994
- Zhang, Ruitian; Suter, Robert M.; Nagle, John F.
- Physical Review E, Vol. 50, Issue 6
Asymmetric Lipid Membranes: Towards More Realistic Model Systems
journal, May 2015
- Marquardt, Drew; Geier, Barbara; Pabst, Georg
- Membranes, Vol. 5, Issue 2
An integrated high-throughput data acquisition system for biological solution X-ray scattering studies
journal, March 2012
- Martel, Anne; Liu, Ping; Weiss, Thomas M.
- Journal of Synchrotron Radiation, Vol. 19, Issue 3
Works referencing / citing this record:
Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy
journal, June 2017
- Zhang, Yuliang; Tunuguntla, Ramya H.; Choi, Pyung-On
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 372, Issue 1726