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Title: Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]

Abstract

Carbon nanotube porins (CNTPs), small segments of carbon nanotubes capable of forming defined pores in lipid membranes, are important future components for bionanoelectronic devices as they could provide a robust analog of biological membrane channels. Furthermore, in order to control the incorporation of these CNT channels into lipid bilayers, it is important to understand the structure of the CNTPs before and after insertion into the lipid bilayer as well as the impact of such insertion on the bilayer structure. Here we employed a noninvasive in situ probe, small-angle X-ray scattering, to study the integration of CNT porins into dioleoylphosphatidylcholine bilayers. These results show that CNTPs in solution are stabilized by a monolayer of lipid molecules wrapped around their outer surface. We also demonstrate that insertion of CNTPs into the lipid bilayer results in decreased bilayer thickness with the magnitude of this effect increasing with the concentration of CNTPs.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1367965
Report Number(s):
LLNL-JRNL-679134
Journal ID: ISSN 1530-6984
Grant/Contract Number:  
AC52-07NA27344; SCW0972; AC02-76SF00515; P41GM103393
Resource Type:
Accepted Manuscript
Journal Name:
Nano Letters
Additional Journal Information:
Journal Volume: 16; Journal Issue: 7; Journal ID: ISSN 1530-6984
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; carbon nanotube; Phospholipid; porins; small-angle X-ray scattering (SAXS)

Citation Formats

Tran, Ich C., Tunuguntla, Ramya H., Kim, Kyunghoon, Lee, Jonathan R. I., Willey, Trevor M., Weiss, Thomas M., Noy, Aleksandr, and van Buuren, Tony. Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]. United States: N. p., 2016. Web. https://doi.org/10.1021/acs.nanolett.6b00466.
Tran, Ich C., Tunuguntla, Ramya H., Kim, Kyunghoon, Lee, Jonathan R. I., Willey, Trevor M., Weiss, Thomas M., Noy, Aleksandr, & van Buuren, Tony. Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]. United States. https://doi.org/10.1021/acs.nanolett.6b00466
Tran, Ich C., Tunuguntla, Ramya H., Kim, Kyunghoon, Lee, Jonathan R. I., Willey, Trevor M., Weiss, Thomas M., Noy, Aleksandr, and van Buuren, Tony. Mon . "Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]". United States. https://doi.org/10.1021/acs.nanolett.6b00466. https://www.osti.gov/servlets/purl/1367965.
@article{osti_1367965,
title = {Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study [Atomic-level structure of carbon nanotube porins in lipid bilayers: an in-situ small-angle x-ray scattering (SAXS) study]},
author = {Tran, Ich C. and Tunuguntla, Ramya H. and Kim, Kyunghoon and Lee, Jonathan R. I. and Willey, Trevor M. and Weiss, Thomas M. and Noy, Aleksandr and van Buuren, Tony},
abstractNote = {Carbon nanotube porins (CNTPs), small segments of carbon nanotubes capable of forming defined pores in lipid membranes, are important future components for bionanoelectronic devices as they could provide a robust analog of biological membrane channels. Furthermore, in order to control the incorporation of these CNT channels into lipid bilayers, it is important to understand the structure of the CNTPs before and after insertion into the lipid bilayer as well as the impact of such insertion on the bilayer structure. Here we employed a noninvasive in situ probe, small-angle X-ray scattering, to study the integration of CNT porins into dioleoylphosphatidylcholine bilayers. These results show that CNTPs in solution are stabilized by a monolayer of lipid molecules wrapped around their outer surface. We also demonstrate that insertion of CNTPs into the lipid bilayer results in decreased bilayer thickness with the magnitude of this effect increasing with the concentration of CNTPs.},
doi = {10.1021/acs.nanolett.6b00466},
journal = {Nano Letters},
number = 7,
volume = 16,
place = {United States},
year = {2016},
month = {6}
}

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