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Title: Tracking microbial interactions with NanoSIMS

The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms. Here, we highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont–host nutrient sharing.
Authors:
 [1] ;  [1] ;  [2] ;  [2]
  1. Helmholtz Centre for Environmental Research - UFZ, Leipzig (Germany)
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Publication Date:
Report Number(s):
LLNL-JRNL-700939
Journal ID: ISSN 0958-1669
Grant/Contract Number:
AC52-07NA27344; SCW1039
Type:
Accepted Manuscript
Journal Name:
Current Opinion in Biotechnology
Additional Journal Information:
Journal Volume: 41; Journal Issue: C; Journal ID: ISSN 0958-1669
Publisher:
Elsevier
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 77 NANOSCIENCE AND NANOTECHNOLOGY
OSTI Identifier:
1366963
Alternate Identifier(s):
OSTI ID: 1434211