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Title: Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation

Mechanical forces in the cell’s natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual cells and cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on cells, particularly with sub-cellular resolution, in controlled in vitro experiments. Here we report a new type of active cell culture material that allows highly localized, directional and reversible deformation of the cell growth substrate, with control at scales ranging from the entire surface to the subcellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single cell micro-manipulation and 2D cell sheet mechanical stimulation techniques.
Authors:
 [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [3] ;  [1] ;  [4] ;  [1] ;  [1]
  1. Harvard Univ., Cambridge, MA (United States)
  2. Harvard Univ., Cambridge, MA (United States); Aalto Univ., Otaniemi (Finland)
  3. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
  4. Harvard Univ., Cambridge, MA (United States); Pennsylvania State Univ., University Park, PA (United States)
Publication Date:
Grant/Contract Number:
SC0005247
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 60 APPLIED LIFE SCIENCES
OSTI Identifier:
1366541

Sutton, Amy, Shirman, Tanya, Timonen, Jaakko V. I., England, Grant T., Kim, Philseok, Kolle, Mathias, Ferrante, Thomas, Zarzar, Lauren D., Strong, Elizabeth, and Aizenberg, Joanna. Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation. United States: N. p., Web. doi:10.1038/ncomms14700.
Sutton, Amy, Shirman, Tanya, Timonen, Jaakko V. I., England, Grant T., Kim, Philseok, Kolle, Mathias, Ferrante, Thomas, Zarzar, Lauren D., Strong, Elizabeth, & Aizenberg, Joanna. Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation. United States. doi:10.1038/ncomms14700.
Sutton, Amy, Shirman, Tanya, Timonen, Jaakko V. I., England, Grant T., Kim, Philseok, Kolle, Mathias, Ferrante, Thomas, Zarzar, Lauren D., Strong, Elizabeth, and Aizenberg, Joanna. 2017. "Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation". United States. doi:10.1038/ncomms14700. https://www.osti.gov/servlets/purl/1366541.
@article{osti_1366541,
title = {Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation},
author = {Sutton, Amy and Shirman, Tanya and Timonen, Jaakko V. I. and England, Grant T. and Kim, Philseok and Kolle, Mathias and Ferrante, Thomas and Zarzar, Lauren D. and Strong, Elizabeth and Aizenberg, Joanna},
abstractNote = {Mechanical forces in the cell’s natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual cells and cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on cells, particularly with sub-cellular resolution, in controlled in vitro experiments. Here we report a new type of active cell culture material that allows highly localized, directional and reversible deformation of the cell growth substrate, with control at scales ranging from the entire surface to the subcellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single cell micro-manipulation and 2D cell sheet mechanical stimulation techniques.},
doi = {10.1038/ncomms14700},
journal = {Nature Communications},
number = ,
volume = 8,
place = {United States},
year = {2017},
month = {3}
}

Works referenced in this record:

Hydrogel-actuated integrated responsive systems (HAIRS): Moving towards adaptive materials
journal, December 2011
  • Kim, Philseok; Zarzar, Lauren D.; He, Ximin
  • Current Opinion in Solid State and Materials Science, Vol. 15, Issue 6, p. 236-245
  • DOI: 10.1016/j.cossms.2011.05.004

Fabrication of Bioinspired Actuated Nanostructures with Arbitrary Geometry and Stiffness
journal, January 2009
  • Pokroy, Boaz; Epstein, Alexander K.; Persson-Gulda, Maria C. M.
  • Advanced Materials, Vol. 21, Issue 4, p. 463-469
  • DOI: 10.1002/adma.200801432

Matrix Elasticity Directs Stem Cell Lineage Specification
journal, August 2006