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Title: Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility

Sustainable utilization of lignocellulosic perennial grass feedstocks will be enabled by high biomass production and optimized cell wall chemistry for efficient conversion into biofuels. MicroRNAs are regulatory elements that modulate the expression of genes involved in various biological functions in plants, including growth and development. In greenhouse studies, overexpressing a microRNA (miR156) gene in switchgrass had dramatic effects on plant architecture and flowering, which appeared to be driven by transgene expression levels. High expressing lines were extremely dwarfed, whereas low and moderate-expressing lines had higher biomass yields, improved sugar release and delayed flowering. Four lines with moderate or low miR156 overexpression from the prior greenhouse study were selected for a field experiment to assess the relationship between miR156 expression and biomass production over three years. We also analysed important bioenergy feedstock traits such as flowering, disease resistance, cell wall chemistry and biofuel production. Phenotypes of the transgenic lines were inconsistent between the greenhouse and the field as well as among different field growing seasons. One low expressing transgenic line consistently produced more biomass (25%–56%) than the control across all three seasons, which translated to the production of 30% more biofuel per plant during the final season. The other three transgenicmore » lines produced less biomass than the control by the final season, and the two lines with moderate expression levels also exhibited altered disease susceptibilities. Results of this study emphasize the importance of performing multiyear field studies for plants with altered regulatory transgenes that target plant growth and development.« less
Authors:
 [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [3] ;  [3] ;  [4] ;  [4] ; ORCiD logo [4] ;  [2] ; ORCiD logo [2] ;  [3] ;  [1]
  1. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Samuel Roberts Noble Foundation, Ardmore, OK (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Report Number(s):
NREL/JA-5100-68393
Journal ID: ISSN 1467-7644
Grant/Contract Number:
AC05-00OR22725; AC36-08GO28308
Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 16; Journal Issue: 1; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; microRNA156; biomass; transgene regulation; switchgrass; flowering; bioconfinement; 09 BIOMASS FUELS; gene expression
OSTI Identifier:
1364675
Alternate Identifier(s):
OSTI ID: 1364676; OSTI ID: 1376550; OSTI ID: 1395091; OSTI ID: 1460175

Baxter, Holly L., Mazarei, Mitra, Dumitrache, Alexandru, Natzke, Jace M., Rodriguez, Miguel, Gou, Jiqing, Fu, Chunxiang, Sykes, Robert W., Turner, Geoffrey B., Davis, Mark F., Brown, Steven D., Davison, Brian H., Wang, Zeng-Yu, and Stewart, Jr., C. Neal. Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility. United States: N. p., Web. doi:10.1111/pbi.12747.
Baxter, Holly L., Mazarei, Mitra, Dumitrache, Alexandru, Natzke, Jace M., Rodriguez, Miguel, Gou, Jiqing, Fu, Chunxiang, Sykes, Robert W., Turner, Geoffrey B., Davis, Mark F., Brown, Steven D., Davison, Brian H., Wang, Zeng-Yu, & Stewart, Jr., C. Neal. Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility. United States. doi:10.1111/pbi.12747.
Baxter, Holly L., Mazarei, Mitra, Dumitrache, Alexandru, Natzke, Jace M., Rodriguez, Miguel, Gou, Jiqing, Fu, Chunxiang, Sykes, Robert W., Turner, Geoffrey B., Davis, Mark F., Brown, Steven D., Davison, Brian H., Wang, Zeng-Yu, and Stewart, Jr., C. Neal. 2017. "Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility". United States. doi:10.1111/pbi.12747.
@article{osti_1364675,
title = {Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility},
author = {Baxter, Holly L. and Mazarei, Mitra and Dumitrache, Alexandru and Natzke, Jace M. and Rodriguez, Miguel and Gou, Jiqing and Fu, Chunxiang and Sykes, Robert W. and Turner, Geoffrey B. and Davis, Mark F. and Brown, Steven D. and Davison, Brian H. and Wang, Zeng-Yu and Stewart, Jr., C. Neal},
abstractNote = {Sustainable utilization of lignocellulosic perennial grass feedstocks will be enabled by high biomass production and optimized cell wall chemistry for efficient conversion into biofuels. MicroRNAs are regulatory elements that modulate the expression of genes involved in various biological functions in plants, including growth and development. In greenhouse studies, overexpressing a microRNA (miR156) gene in switchgrass had dramatic effects on plant architecture and flowering, which appeared to be driven by transgene expression levels. High expressing lines were extremely dwarfed, whereas low and moderate-expressing lines had higher biomass yields, improved sugar release and delayed flowering. Four lines with moderate or low miR156 overexpression from the prior greenhouse study were selected for a field experiment to assess the relationship between miR156 expression and biomass production over three years. We also analysed important bioenergy feedstock traits such as flowering, disease resistance, cell wall chemistry and biofuel production. Phenotypes of the transgenic lines were inconsistent between the greenhouse and the field as well as among different field growing seasons. One low expressing transgenic line consistently produced more biomass (25%–56%) than the control across all three seasons, which translated to the production of 30% more biofuel per plant during the final season. The other three transgenic lines produced less biomass than the control by the final season, and the two lines with moderate expression levels also exhibited altered disease susceptibilities. Results of this study emphasize the importance of performing multiyear field studies for plants with altered regulatory transgenes that target plant growth and development.},
doi = {10.1111/pbi.12747},
journal = {Plant Biotechnology Journal},
number = 1,
volume = 16,
place = {United States},
year = {2017},
month = {4}
}

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