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Title: RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production

Sugarcane is a subtropical crop that produces large amounts of biomass annually. It is a key agricultural crop in many countries for the production of sugar and other products. Residual bagasse following sucrose extraction is currently underutilized and it has potential as a carbohydrate source for the production of biofuels. As with all lignocellulosic crops, lignin acts as a barrier to accessing the polysaccharides, and as such, is the focus of transgenic efforts. In this study, we used RNAi to individually reduce the expression of three key genes in the lignin biosynthetic pathway in sugarcane. Furthermore, these genes, caffeoyl-CoA O-methyltransferase ( CCoAOMT), ferulate 5-hydroxylase ( F5H) and caffeic acid O-methyltransferase ( COMT), impact lignin content and/or composition.
Authors:
 [1] ; ORCiD logo [2] ; ORCiD logo [3] ; ORCiD logo [3] ; ORCiD logo [2]
  1. Queensland Univ. of Technology, Brisbane, QLD (Australia)
  2. Syracuse Univ., Syracuse, NY (United States)
  3. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Grant/Contract Number:
FC02-07ER64494
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Syracuse Univ., Syracuse, NY (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; lignin biosynthesis; ferulate 5-hydroxylase; caffeic acid O-methyltransferase; caffeoyl-CoA O-methyltransferase; sugarcane; RNAi
OSTI Identifier:
1363952