Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production
Abstract
Summary Microbial cell wall‐deconstructing enzymes are widely used in the food, wine, pulp and paper, textile, and detergent industries and will be heavily utilized by cellulosic biorefineries in the production of fuels and chemicals. Due to their ability to use freely available solar energy, genetically engineered bioenergy crops provide an attractive alternative to microbial bioreactors for the production of cell wall‐deconstructing enzymes. This review article summarizes the efforts made within the last decade on the production of cell wall‐deconstructing enzymes in planta for use in the deconstruction of lignocellulosic biomass. A number of strategies have been employed to increase enzyme yields and limit negative impacts on plant growth and development including targeting heterologous enzymes into specific subcellular compartments using signal peptides, using tissue‐specific or inducible promoters to limit the expression of enzymes to certain portions of the plant or certain times, and fusion of amplification sequences upstream of the coding region to enhance expression. We also summarize methods that have been used to access and maintain activity of plant‐generated enzymes when used in conjunction with thermochemical pretreatments for the production of lignocellulosic biofuels.
- Authors:
-
[2];
- Department of Plant, Soil and Microbial Sciences Michigan State University East Lansing MI USA
- Department of Chemical Engineering and Materials Science DOE Great Lakes Bioenergy Research Center Michigan State University Lansing MI USA, Department of Chemical Engineering Michigan Technological University Houghton MI USA
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1227588
- Alternate Identifier(s):
- OSTI ID: 1227591; OSTI ID: 1363935
- Grant/Contract Number:
- FC02-07ER64494
- Resource Type:
- Published Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 14 Journal Issue: 6; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; cell wall-deconstructing enzymes; lignocellulosic biomass; biofuels; promoter; plant subcellular compartments; signal peptides
Citation Formats
Park, Sang‐Hyuck, Ong, Rebecca Garlock, and Sticklen, Mariam. Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production. United Kingdom: N. p., 2015.
Web. doi:10.1111/pbi.12505.
Park, Sang‐Hyuck, Ong, Rebecca Garlock, & Sticklen, Mariam. Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production. United Kingdom. https://doi.org/10.1111/pbi.12505
Park, Sang‐Hyuck, Ong, Rebecca Garlock, and Sticklen, Mariam. Wed .
"Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production". United Kingdom. https://doi.org/10.1111/pbi.12505.
@article{osti_1227588,
title = {Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production},
author = {Park, Sang‐Hyuck and Ong, Rebecca Garlock and Sticklen, Mariam},
abstractNote = {Summary Microbial cell wall‐deconstructing enzymes are widely used in the food, wine, pulp and paper, textile, and detergent industries and will be heavily utilized by cellulosic biorefineries in the production of fuels and chemicals. Due to their ability to use freely available solar energy, genetically engineered bioenergy crops provide an attractive alternative to microbial bioreactors for the production of cell wall‐deconstructing enzymes. This review article summarizes the efforts made within the last decade on the production of cell wall‐deconstructing enzymes in planta for use in the deconstruction of lignocellulosic biomass. A number of strategies have been employed to increase enzyme yields and limit negative impacts on plant growth and development including targeting heterologous enzymes into specific subcellular compartments using signal peptides, using tissue‐specific or inducible promoters to limit the expression of enzymes to certain portions of the plant or certain times, and fusion of amplification sequences upstream of the coding region to enhance expression. We also summarize methods that have been used to access and maintain activity of plant‐generated enzymes when used in conjunction with thermochemical pretreatments for the production of lignocellulosic biofuels.},
doi = {10.1111/pbi.12505},
journal = {Plant Biotechnology Journal},
number = 6,
volume = 14,
place = {United Kingdom},
year = {Wed Dec 02 00:00:00 EST 2015},
month = {Wed Dec 02 00:00:00 EST 2015}
}
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In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
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Heterologous Acidothermus cellulolyticus 1,4-β-endoglucanase E1 produced within the corn biomass converts corn stover into glucose
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Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides
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Assessment of field-grown cellulase-expressing corn
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Hyperthermophilic endoglucanase for in planta lignocellulose conversion
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Identification of Novel Plant Peroxisomal Targeting Signals by a Combination of Machine Learning Methods and in Vivo Subcellular Targeting Analyses
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Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea)
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Microbial Cellulases and Their Industrial Applications
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Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes
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Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon Pyrococcus horikoshii by Tobacco Chloroplast Engineering
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Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
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Optimization of Acidothermus cellulolyticus Endoglucanase (E1) Production in Transgenic Tobacco Plants by Transcriptional, Post-transcription and Post-translational Modification
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Effects of an exogenous xylanase gene expression on the growth of transgenic rice and the expression level of endogenous xylanase inhibitor gene RIXI : Rice expressing an exogenous xylanase gene
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Stable expression of a thermostable xylanase of Clostridium thermocellum in cultured tobacco cells
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Analysis of five novel putative constitutive gene promoters in transgenic rice plants
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Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast
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Controlled expression of pectic enzymes in Arabidopsis thaliana enhances biomass conversion without adverse effects on growth
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Arabidopsis and Brachypodium distachyon Transgenic Plants Expressing Aspergillus nidulans Acetylesterases Have Decreased Degree of Polysaccharide Acetylation and Increased Resistance to Pathogens
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Expression of xylanase with high specific activity from Streptomyces olivaceoviridis A1 in transgenic potato plants (Solanum tuberosum L.)
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High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome
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Hydrophobin Fusions for High-Level Transient Protein Expression and Purification in Nicotiana benthamiana
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Targeting a heterologous protein to multiple plant organelles via rationally designed 5[prime] mRNA tags
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Expression of a Trichoderma reesei β-1,4 endo-xylanase in tall fescue modifies cell wall structure and digestibility and elicits pathogen defence responses
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