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Title: Optimization of one-step in situ transesterification method for accurate quantification of EPA in Nannochloropsis gaditana

Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA) due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA), thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 °C; 1.5 h), the maximum fatty acid methyl ester (FAME) yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58- and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform). Furthermore, this one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME) yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [3]
  1. Nanjing Forestry Univ., Nanjing (China); Johns Hopkins Univ., Baltimore, MD (United States)
  2. Johns Hopkins Univ., Baltimore, MD (United States)
  3. Nanjing Forestry Univ., Nanjing (China)
Publication Date:
Grant/Contract Number:
SC0012658
Type:
Accepted Manuscript
Journal Name:
Applied Sciences
Additional Journal Information:
Journal Volume: 6; Journal Issue: 11; Journal ID: ISSN 2076-3417
Publisher:
MDPI
Research Org:
Johns Hopkins Univ., Baltimore, MD (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; EPA; FAME; one-step in situ transesterification; Nannochloropsis gaditana
OSTI Identifier:
1362285

Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Betenbaugh, Michael J., and Wang, Fei. Optimization of one-step in situ transesterification method for accurate quantification of EPA in Nannochloropsis gaditana. United States: N. p., Web. doi:10.3390/app6110343.
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Betenbaugh, Michael J., & Wang, Fei. Optimization of one-step in situ transesterification method for accurate quantification of EPA in Nannochloropsis gaditana. United States. doi:10.3390/app6110343.
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Betenbaugh, Michael J., and Wang, Fei. 2016. "Optimization of one-step in situ transesterification method for accurate quantification of EPA in Nannochloropsis gaditana". United States. doi:10.3390/app6110343. https://www.osti.gov/servlets/purl/1362285.
@article{osti_1362285,
title = {Optimization of one-step in situ transesterification method for accurate quantification of EPA in Nannochloropsis gaditana},
author = {Tang, Yuting and Zhang, Yue and Rosenberg, Julian N. and Betenbaugh, Michael J. and Wang, Fei},
abstractNote = {Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA) due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA), thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 °C; 1.5 h), the maximum fatty acid methyl ester (FAME) yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58- and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform). Furthermore, this one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME) yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae.},
doi = {10.3390/app6110343},
journal = {Applied Sciences},
number = 11,
volume = 6,
place = {United States},
year = {2016},
month = {11}
}