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Title: Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa

Abstract

The bacterium Clostridium thermocellum offers a distinct and integrated approach to ethanol production through consolidated bioprocessing (CBP). The Simons’ stain technique, which assays the accessibility of lignocellulosic biomass, has been traditionally applied to fungal cellulase systems; however, its application to CBP has not been fully explored. For this reason, the structural properties of eight Populus trichocarpa with either high or low biomass densities were compared in this paper to determine bioconversion differences during separate hydrolysis and fermentation (SHF) and CBP with C. thermocellum. Simons’ staining generally identifies low density poplar as more accessible than high density poplar. Additionally, low density P. trichocarpa generally contained less Klason lignin than high density poplar. SHF and CBP treatments consistently identified BESC-7 (high density, low accessibility, low surface roughness) as a low ethanol yielding biomass and GW-9914 (low density, high accessibility, high surface roughness) as a high ethanol yielding biomass. Upon further investigation, BESC-7 also contained a high Klason lignin content (~25%), while GW-9914 had a low lignin content (~20%). Cellulose degree of polymerization (DP) measurements exhibited a weak linear correlation with accessibility (r2 = 0.17). Finally, therefore, the ethanol yields were correlated with accessibility and lignin content extremes but not cellulose DP.

Authors:
 [1];  [2];  [2];  [3];  [3];  [3];  [2]; ORCiD logo [4]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Georgia Inst. of Technology, Atlanta, GA (United States). School of Chemistry and Biochemistry. Renewable BioProducts Inst.
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center. Biosciences Division. UT-ORNL Joint Inst. of Biological Science
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioSciences Division. Plant Systems Biology Group
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center. Biosciences Division. UT-ORNL Joint Inst. of Biological Science; Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon. Dept. of Forestry. Dept. of Chemical and Biomolecular Engineering
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Contributing Org.:
Georgia Inst. of Technology, Atlanta, GA (United States); Univ. of Tennessee, Knoxville, TN (United States)
OSTI Identifier:
1362259
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
ACS Sustainable Chemistry & Engineering
Additional Journal Information:
Journal Volume: 5; Journal Issue: 6; Journal ID: ISSN 2168-0485
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Clostridium thermocellum; consolidated bioprocessing; ethanol; Klason lignin; Simons’ stain; wood density

Citation Formats

Akinosho, Hannah, Dumitrache, Alexandru, Natzke, Jace, Muchero, Wellington, Jawdy, Sara S., Tuskan, Gerald A., Brown, Steven D., and Ragauskas, Arthur J.. Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa. United States: N. p., 2017. Web. doi:10.1021/acssuschemeng.7b00449.
Akinosho, Hannah, Dumitrache, Alexandru, Natzke, Jace, Muchero, Wellington, Jawdy, Sara S., Tuskan, Gerald A., Brown, Steven D., & Ragauskas, Arthur J.. Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa. United States. https://doi.org/10.1021/acssuschemeng.7b00449
Akinosho, Hannah, Dumitrache, Alexandru, Natzke, Jace, Muchero, Wellington, Jawdy, Sara S., Tuskan, Gerald A., Brown, Steven D., and Ragauskas, Arthur J.. Wed . "Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa". United States. https://doi.org/10.1021/acssuschemeng.7b00449. https://www.osti.gov/servlets/purl/1362259.
@article{osti_1362259,
title = {Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa},
author = {Akinosho, Hannah and Dumitrache, Alexandru and Natzke, Jace and Muchero, Wellington and Jawdy, Sara S. and Tuskan, Gerald A. and Brown, Steven D. and Ragauskas, Arthur J.},
abstractNote = {The bacterium Clostridium thermocellum offers a distinct and integrated approach to ethanol production through consolidated bioprocessing (CBP). The Simons’ stain technique, which assays the accessibility of lignocellulosic biomass, has been traditionally applied to fungal cellulase systems; however, its application to CBP has not been fully explored. For this reason, the structural properties of eight Populus trichocarpa with either high or low biomass densities were compared in this paper to determine bioconversion differences during separate hydrolysis and fermentation (SHF) and CBP with C. thermocellum. Simons’ staining generally identifies low density poplar as more accessible than high density poplar. Additionally, low density P. trichocarpa generally contained less Klason lignin than high density poplar. SHF and CBP treatments consistently identified BESC-7 (high density, low accessibility, low surface roughness) as a low ethanol yielding biomass and GW-9914 (low density, high accessibility, high surface roughness) as a high ethanol yielding biomass. Upon further investigation, BESC-7 also contained a high Klason lignin content (~25%), while GW-9914 had a low lignin content (~20%). Cellulose degree of polymerization (DP) measurements exhibited a weak linear correlation with accessibility (r2 = 0.17). Finally, therefore, the ethanol yields were correlated with accessibility and lignin content extremes but not cellulose DP.},
doi = {10.1021/acssuschemeng.7b00449},
journal = {ACS Sustainable Chemistry & Engineering},
number = 6,
volume = 5,
place = {United States},
year = {Wed Apr 26 00:00:00 EDT 2017},
month = {Wed Apr 26 00:00:00 EDT 2017}
}

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Works referenced in this record:

Do Enzymatic Hydrolyzability and Simons' Stain Reflect the Changes in the Accessibility of Lignocellulosic Substrates to Cellulase Enzymes?
journal, December 2001

  • Esteghlalian, A. R.; Bilodeau, M.; Mansfield, S. D.
  • Biotechnology Progress, Vol. 17, Issue 6
  • DOI: 10.1021/bp0101177

Synergistic interactions in cellulose hydrolysis
journal, January 2012


The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility
journal, January 2012


A staining technique for evaluating the pore structure variations of microcrystalline cellulose powders
journal, August 1998


Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates
journal, January 2011

  • Arantes, Valdeir; Saddler, Jack N.
  • Biotechnology for Biofuels, Vol. 4, Issue 1, Article No. 3
  • DOI: 10.1186/1754-6834-4-3

Steam pretreatment of agricultural residues facilitates hemicellulose recovery while enhancing enzyme accessibility to cellulose
journal, June 2015


How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure
journal, February 2016

  • Dumitrache, Alexandru; Akinosho, Hannah; Rodriguez, Miguel
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0445-x

Porosity and pore size distribution of different wood types as determined by mercury intrusion porosimetry
journal, November 2010


Effect of Wood Preservatives on Surface Properties of Coated Wood
journal, January 2015

  • Ozdemir, Turgay; Temiz, Ali; Aydin, Ismail
  • Advances in Materials Science and Engineering, Vol. 2015
  • DOI: 10.1155/2015/631835

High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus
journal, January 2015


The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique
journal, September 2008

  • Chandra, Richard; Ewanick, Shannon; Hsieh, Carmen
  • Biotechnology Progress, Vol. 24, Issue 5
  • DOI: 10.1002/btpr.33

The Influence of Wood Anatomy on Evaluating the Roughness of Sanded Solid Wood
journal, October 2005

  • Gurau, L.; Mansfield-Williams, H.; Irle, M.
  • Journal of the Institute of Wood Science, Vol. 17, Issue 2
  • DOI: 10.1179/wsc.2005.17.2.65

Comparison of laboratory delignification methods, their selectivity, and impacts on physiochemical characteristics of cellulosic biomass
journal, February 2013


Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment
journal, October 2014


Determination of porosity of lignocellulosic biomass before and after pretreatment by using Simons’ stain and NMR techniques
journal, September 2013


Physicochemical Structural Changes of Poplar and Switchgrass during Biomass Pretreatment and Enzymatic Hydrolysis
journal, July 2016


A critical review of analytical methods in pretreatment of lignocelluloses: Composition, imaging, and crystallinity
journal, January 2016


Adsorption of Clostridium thermocellum cellulases onto pretreated mixed hardwood, avicel, and lignin
journal, September 1993

  • Bernardez, Timothy D.; Lyford, Kimberly; Hogsett, David A.
  • Biotechnology and Bioengineering, Vol. 42, Issue 7, p. 899-907
  • DOI: 10.1002/bit.260420715

Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae
journal, September 2009

  • Ding, Junmei; Huang, Xiaowei; Zhang, Lemin
  • Applied Microbiology and Biotechnology, Vol. 85, Issue 2
  • DOI: 10.1007/s00253-009-2223-1

Enhanced ethanol production via electrostatically accelerated fermentation of glucose using Saccharomyces cerevisiae
journal, October 2015

  • Mathew, Anup Sam; Wang, Jiapeng; Luo, Jieling
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep15713

Lignin content in natural Populus variants affects sugar release
journal, March 2011

  • Studer, M. H.; DeMartini, J. D.; Davis, M. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
  • DOI: 10.1073/pnas.1009252108

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellum strain
journal, January 2014

  • Yee, Kelsey L.; Rodriguez Jr, Miguel; Thompson, Olivia A.
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/1754-6834-7-75

Cellulose and lignin colocalization at the plant cell wall surface limits microbial hydrolysis of Populus biomass
journal, January 2017

  • Dumitrache, Alexandru; Tolbert, Allison; Natzke, Jace
  • Green Chemistry, Vol. 19, Issue 9
  • DOI: 10.1039/C7GC00346C

Cell wall fermentation kinetics are impacted more by lignin content and ferulate cross-linking than by lignin composition
journal, January 2009

  • Grabber, John H.; Mertens, David R.; Kim, Hoon
  • Journal of the Science of Food and Agriculture, Vol. 89, Issue 1
  • DOI: 10.1002/jsfa.3418

Works referencing / citing this record:

Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum
journal, August 2018

  • Kothari, Ninad; Holwerda, Evert K.; Cai, Charles M.
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1216-7