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Title: MultispeQ Beta: a tool for large-scale plant phenotyping connected to the open PhotosynQ network

Large-scale high-throughput plant phenotyping (sometimes called phenomics) is becoming increasingly important in plant biology and agriculture and is essential to cutting-edge plant breeding and management approaches needed to meet the food and fuel needs for the next century. Currently, the application of these approaches is severely limited by the availability of appropriate instrumentation and by the ability to communicate experimental protocols, results and analyses. To address these issues, we have developed a low-cost, yet sophisticated open-source scientific instrument designed to enable communities of researchers, plant breeders, educators, farmers and citizen scientists to collect high-quality field data on a large scale. The MultispeQ provides measurements in the field or laboratory of both, environmental conditions (light intensity and quality, temperature, humidity, CO 2 levels, time and location) and useful plant phenotypes, including photosynthetic parameters—photosystem II quantum yield (Φ II), non-photochemical exciton quenching (NPQ), photosystem II photoinhibition, light-driven proton translocation and thylakoid proton motive force, regulation of the chloroplast ATP synthase and potentially many others—and leaf chlorophyll and other pigments. Plant phenotype data are transmitted from the MultispeQ to mobile devices, laptops or desktop computers together with key metadata that gets saved to the PhotosynQ platform ( and provides a suite of web-basedmore » tools for sharing, visualization, filtering, dissemination and analyses. We present validation experiments, comparing MultispeQ results with established platforms, and show that it can be usefully deployed in both laboratory and field settings. We present evidence that MultispeQ can be used by communities of researchers to rapidly measure, store and analyse multiple environmental and plant properties, allowing for deeper understanding of the complex interactions between plants and their environment.« less
ORCiD logo [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [1] ;  [1] ;  [3] ;  [1]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE-Plant Research Lab.
  2. Michigan State Univ., East Lansing, MI (United States). Dept. of Plant, Soil and Microbial Sciences. Genetics Graduate Program
  3. Venturit Inc., East Lansing, MI (United States)
Publication Date:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Royal Society Open Science
Additional Journal Information:
Journal Volume: 3; Journal Issue: 10; Journal ID: ISSN 2054-5703
Research Org:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); United States Agency for International Development (USAID); McKnight Foundation (United States)
Contributing Orgs:
Venturit Inc., East Lansing, MI (United States)
Country of Publication:
United States
97 MATHEMATICS AND COMPUTING; 47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES; agriculture; chlorophyll fluorescence; open-source hardware; phenomics; photosynthesis
OSTI Identifier: