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Title: Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy

Functional changes in rat kidneys during the induced ischemic injury and recovery phases were explored using multimodal autofluorescence and light scattering imaging. We aim to evaluate the use of noncontact optical signatures for rapid assessment of tissue function and viability. Specifically, autofluorescence images were acquired in vivo under 355, 325, and 266 nm illumination while light scattering images were collected at the excitation wavelengths as well as using relatively narrowband light centered at 500 nm. The images were simultaneously recorded using a multimodal optical imaging system. We also analyzed to obtain time constants, which were correlated to kidney dysfunction as determined by a subsequent survival study and histopathological analysis. This analysis of both the light scattering and autofluorescence images suggests that changes in tissue microstructure, fluorophore emission, and blood absorption spectral characteristics, coupled with vascular response, contribute to the behavior of the observed signal, which may be used to obtain tissue functional information and offer the ability to predict posttransplant kidney function.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Davis, CA (United States). Center for Biophotonics Science and Technology
  2. Univ. of California, Davis, CA (United States). Davis Medical Center
  3. California Northstate Univ. College of Medicine, Elk Grove, CA (United States)
Publication Date:
Report Number(s):
LLNL-JRNL-694546
Journal ID: ISSN 1083-3668
Grant/Contract Number:
AC52-07NA27344
Type:
Accepted Manuscript
Journal Name:
Journal of Biomedical Optics
Additional Journal Information:
Journal Volume: 22; Journal Issue: 5; Journal ID: ISSN 1083-3668
Publisher:
SPIE
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE; National Science Foundation (NSF)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1361609

Raman, Rajesh N., Pivetti, Christopher D., Ramsamooj, Rajendra, Troppmann, Christoph, and Demos, Stavros G.. Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy. United States: N. p., Web. doi:10.1117/1.JBO.22.5.056001.
Raman, Rajesh N., Pivetti, Christopher D., Ramsamooj, Rajendra, Troppmann, Christoph, & Demos, Stavros G.. Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy. United States. doi:10.1117/1.JBO.22.5.056001.
Raman, Rajesh N., Pivetti, Christopher D., Ramsamooj, Rajendra, Troppmann, Christoph, and Demos, Stavros G.. 2017. "Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy". United States. doi:10.1117/1.JBO.22.5.056001. https://www.osti.gov/servlets/purl/1361609.
@article{osti_1361609,
title = {Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy},
author = {Raman, Rajesh N. and Pivetti, Christopher D. and Ramsamooj, Rajendra and Troppmann, Christoph and Demos, Stavros G.},
abstractNote = {Functional changes in rat kidneys during the induced ischemic injury and recovery phases were explored using multimodal autofluorescence and light scattering imaging. We aim to evaluate the use of noncontact optical signatures for rapid assessment of tissue function and viability. Specifically, autofluorescence images were acquired in vivo under 355, 325, and 266 nm illumination while light scattering images were collected at the excitation wavelengths as well as using relatively narrowband light centered at 500 nm. The images were simultaneously recorded using a multimodal optical imaging system. We also analyzed to obtain time constants, which were correlated to kidney dysfunction as determined by a subsequent survival study and histopathological analysis. This analysis of both the light scattering and autofluorescence images suggests that changes in tissue microstructure, fluorophore emission, and blood absorption spectral characteristics, coupled with vascular response, contribute to the behavior of the observed signal, which may be used to obtain tissue functional information and offer the ability to predict posttransplant kidney function.},
doi = {10.1117/1.JBO.22.5.056001},
journal = {Journal of Biomedical Optics},
number = 5,
volume = 22,
place = {United States},
year = {2017},
month = {5}
}