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Title: Glycomic and glycoproteomic analysis of glycoproteins—a tutorial

The structural analysis of glycoproteins is a challenging endeavor and is under steadily increasing demand, but only a very limited number of labs have the expertise required to accomplish this task. This tutorial is aimed at researchers from the fields of molecular biology and biochemistry that have discovered that glycoproteins are important in their biological research and are looking for the tools to elucidate their structure. It provides brief descriptions of the major and most common analytical techniques used in glycomics and glycoproteomics analysis, including explanations of the rationales for individual steps and references to published literature containing the experimental details necessary to carry out the analyses. Glycomics includes the comprehensive study of the structure and function of the glycans expressed in a given cell or organism along with identification of all the genes that encode glycoproteins and glycosyltransferases. Glycoproteomics which is subset of both glycomics and proteomics is the identification and characterization of proteins bearing carbohydrates as posttranslational modification. This tutorial is designed to ease entry into the glycomics and glycoproteomics field for those without prior carbohydrate analysis experience.
Authors:
 [1] ;  [1] ;  [1] ;  [1]
  1. Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
Publication Date:
Grant/Contract Number:
SC0015662
Type:
Published Article
Journal Name:
Analytical and Bioanalytical Chemistry
Additional Journal Information:
Journal Volume: 409; Journal Issue: 19; Journal ID: ISSN 1618-2642
Publisher:
Springer
Research Org:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Glycomics; Glycoproteomics; Mass spectrometry; Glycan analysis; Glycopeptide; Glycosylation site mapping
OSTI Identifier:
1361436
Alternate Identifier(s):
OSTI ID: 1426154