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Title: Characterization of a novel, ubiquitous fungal endophyte from the rhizosphere and root endosphere of Populus trees

Here, we examined variation in growth rate, patterns of nitrogen utilization, and competitive interactions of Atractiellarhizophila isolates from the roots of Populus hosts. Atractiella grew significantly faster on media substituted with inorganic nitrogen sources and slower in the presence of another fungal genus. In order to determine plausible causal mechanisms we used metabolomics to explore competitive interactions between Atractiella strains and Fusarium oxysporum or Leptosphaerulina chartarum. Metabolomic screening of potential microbial inhibitors showed increased levels of glycosides produced in vitro by Atractiella when grown with a different fungal genus, relative to when grown alone. Overall, our results suggest Atractiella is a poor competitor with other fungi via direct routes e.g. faster growth rates, but may utilize chemical interactions and possibly nitrogen sources to defend itself, and niche partition its way to abundance in the plant host root and rhizosphere.
Authors:
 [1] ;  [1] ;  [2] ;  [1] ;  [2] ;  [3] ;  [2] ;  [1] ;  [1] ;  [4]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  2. Duke Univ., Durham, NC (United States). Dept. of Biology
  3. (United States). Dept. of Plant, Soil and Microbial Sciences
  4. (United States). Dept. of Civil and Environmental Engineering
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Fungal Ecology
Additional Journal Information:
Journal Volume: 27; Journal Issue: PA; Journal ID: ISSN 1754-5048
Publisher:
Elsevier
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Atractiella; Fungal endophytes; Competition; Metabolomics; Organic and inorganic nitrogen; Symbiosis; Pathogen; Mutualist
OSTI Identifier:
1361315
Alternate Identifier(s):
OSTI ID: 1415540

Vélez, Jessica M., Tschaplinski, Timothy J., Vilgalys, Rytas, Schadt, Christopher W., Bonito, Gregory, Michigan State Univ., East Lansing, MI, Hameed, Khalid, Engle, Nancy, Hamilton, Cyd E., and Univ. of Tennessee, Knoxville, TN. Characterization of a novel, ubiquitous fungal endophyte from the rhizosphere and root endosphere of Populus trees. United States: N. p., Web. doi:10.1016/j.funeco.2017.03.001.
Vélez, Jessica M., Tschaplinski, Timothy J., Vilgalys, Rytas, Schadt, Christopher W., Bonito, Gregory, Michigan State Univ., East Lansing, MI, Hameed, Khalid, Engle, Nancy, Hamilton, Cyd E., & Univ. of Tennessee, Knoxville, TN. Characterization of a novel, ubiquitous fungal endophyte from the rhizosphere and root endosphere of Populus trees. United States. doi:10.1016/j.funeco.2017.03.001.
Vélez, Jessica M., Tschaplinski, Timothy J., Vilgalys, Rytas, Schadt, Christopher W., Bonito, Gregory, Michigan State Univ., East Lansing, MI, Hameed, Khalid, Engle, Nancy, Hamilton, Cyd E., and Univ. of Tennessee, Knoxville, TN. 2017. "Characterization of a novel, ubiquitous fungal endophyte from the rhizosphere and root endosphere of Populus trees". United States. doi:10.1016/j.funeco.2017.03.001. https://www.osti.gov/servlets/purl/1361315.
@article{osti_1361315,
title = {Characterization of a novel, ubiquitous fungal endophyte from the rhizosphere and root endosphere of Populus trees},
author = {Vélez, Jessica M. and Tschaplinski, Timothy J. and Vilgalys, Rytas and Schadt, Christopher W. and Bonito, Gregory and Michigan State Univ., East Lansing, MI and Hameed, Khalid and Engle, Nancy and Hamilton, Cyd E. and Univ. of Tennessee, Knoxville, TN},
abstractNote = {Here, we examined variation in growth rate, patterns of nitrogen utilization, and competitive interactions of Atractiellarhizophila isolates from the roots of Populus hosts. Atractiella grew significantly faster on media substituted with inorganic nitrogen sources and slower in the presence of another fungal genus. In order to determine plausible causal mechanisms we used metabolomics to explore competitive interactions between Atractiella strains and Fusarium oxysporum or Leptosphaerulina chartarum. Metabolomic screening of potential microbial inhibitors showed increased levels of glycosides produced in vitro by Atractiella when grown with a different fungal genus, relative to when grown alone. Overall, our results suggest Atractiella is a poor competitor with other fungi via direct routes e.g. faster growth rates, but may utilize chemical interactions and possibly nitrogen sources to defend itself, and niche partition its way to abundance in the plant host root and rhizosphere.},
doi = {10.1016/j.funeco.2017.03.001},
journal = {Fungal Ecology},
number = PA,
volume = 27,
place = {United States},
year = {2017},
month = {4}
}