Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst. Biosciences Division. Stanford Synchrotron Radiation Lightsource
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst. Biosciences Division
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst.
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source. Biosciences Division
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Biosciences Division
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource; Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics
We provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination. Se-B SA was chosen as the model system for its high affinity between biotin and streptavidin where the sulfur atom in the biotin molecule (C10H16N2O3S) is substituted with selenium. The dataset was collected at three different transmissions (100, 50, and 10%) using a serial sample chamber setup which allows for two sample chambers, a front chamber and a back chamber, to operate simultaneously. Diffraction patterns from Se-B SA were recorded to a resolution of 1.9 Å. The dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) and also on LCLS compute nodes as a resource for research and algorithm development.
- Research Organization:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Laboratory Directed Research and Development (LDRD) Program; National Inst. of Health (NIH) (United States)
- Contributing Organization:
- Univ. of California, San Francisco, CA (United States)
- Grant/Contract Number:
- AC02-76SF00515; P41GM103393; P41RR001209
- OSTI ID:
- 1361134
- Journal Information:
- Scientific Data, Vol. 4; ISSN 2052-4463
- Publisher:
- Nature Publishing GroupCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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