Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

Abstract

© 2017 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.

Authors:
 [1];  [1];  [1];  [2];  [1];  [3];  [3];  [1];  [1];  [4]; ORCiD logo [5];  [6];  [1];  [7];  [6];  [8];  [6];  [9];  [10];  [9] more »;  [11];  [12];  [13];  [1];  [14];  [4];  [15];  [16];  [17];  [1]; ORCiD logo [18];  [13];  [18];  [18]; ORCiD logo [18];  [18];  [18]; ORCiD logo [18]; ORCiD logo [19];  [18];  [10]; ORCiD logo [18];  [6];  [20]; ORCiD logo [9];  [21];  [21];  [21];  [22];  [23];  [2];  [1];  [13]; ORCiD logo [24];  [1]; ORCiD logo [1] « less
+ Show Author Affiliations
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
  2. Washington Univ., St. Louis, MO (United States). Dept. of Biology
  3. Stockholm Univ. (Sweden). Dept. of Biochemistry and Biophysics
  4. Rice Univ., Houston, TX (United States). Dept. of BioSciences
  5. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
  6. Humboldt Univ. of Berlin (Germany). Inst. fur Biologie
  7. Umea Univ. (Sweden). Inst. for Kemi, Kemiskt Biologiskt Centrum
  8. Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemistry
  9. SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE)
  10. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE); SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  12. Helmholtz-Zentrum Berlin (HZB), (Germany). German Research Centre for Materials and Energy, Inst. for Methods and Instrumentation on Synchrotron Radiation Research
  13. Science and Technology Facilities Council (STFC), Harwell Campus, Oxford (United Kingdom). Diamond Light Source, Ltd.
  14. Science and Technology Facilities Council (STFC), Harwell Campus, Oxford (United Kingdom). Diamond Light Source, Ltd.; Univ. of Oxford (United Kingdom). Dept. of Biochemistry
  15. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; Zewail City of Science and Technology, Giza (Egypt). Center for Photonics and Smart Materials (CPSM)
  16. Umea Univ. (Sweden). Inst. for Kemi, Kemiskt Biologiskt Centrum; SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE)
  17. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II); Ventana Medical Systems, Inc., Tucson, AZ (United States)
  18. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  19. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS); Paul Scherrer Inst. (PSI), Villigen (Switzerland). SwissFEL
  20. Umea Univ. (Sweden). Inst. for Kemi, Kemiskt Biologiskt Centrum; Uppsala Univ. (Sweden). Dept. of Chemistry - Angstrom, Molecular Biomimetics
  21. Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemistry; Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology
  22. Stockholm Univ. (Sweden). Dept. of Biochemistry and Biophysics; Stanford Univ., CA (United States). Dept. of Chemistry
  23. Rice Univ., Houston, TX (United States). Dept. of BioSciences; Rice Univ., Houston, TX (United States). Dept. of Chemistry
  24. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); National Institutes of Health (NIH)
OSTI Identifier:
1360909
Alternate Identifier(s):
OSTI ID: 1379743; OSTI ID: 1392209
Report Number(s):
BNL-114091-2017-JA
Journal ID: ISSN 1548-7091; nmeth.4195
Grant/Contract Number:  
AC02-76SF00515; AC02-05CH11231; AC02-98CH10886; SC0012704; GM110501; P41GM111244; P41GM103393; 2013-541; 2013-5884; 8P41GM103473-16; 2-P41-RR012408
Resource Type:
Accepted Manuscript
Journal Name:
Nature Methods
Additional Journal Information:
Journal Volume: 14; Journal Issue: 4; Journal ID: ISSN 1548-7091
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biocatalysis; Biophysical methods; Enzymes; Molecular biophysics; Nanocrystallography

Citation Formats

Fuller, Franklin D., Gul, Sheraz, Chatterjee, Ruchira, Burgie, E. Sethe, Young, Iris D., Lebrette, Hugo, Srinivas, Vivek, Brewster, Aaron S., Michels-Clark, Tara, Clinger, Jonathan A., Andi, Babak, Ibrahim, Mohamed, Pastor, Ernest, de Lichtenberg, Casper, Hussein, Rana, Pollock, Christopher J., Zhang, Miao, Stan, Claudiu A., Kroll, Thomas, Fransson, Thomas, Weninger, Clemens, Kubin, Markus, Aller, Pierre, Lassalle, Louise, Bräuer, Philipp, Miller, Mitchell D., Amin, Muhamed, Koroidov, Sergey, Roessler, Christian G., Allaire, Marc, Sierra, Raymond G., Docker, Peter T., Glownia, James M., Nelson, Silke, Koglin, Jason E., Zhu, Diling, Chollet, Matthieu, Song, Sanghoon, Lemke, Henrik, Liang, Mengning, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Zouni, Athina, Messinger, Johannes, Bergmann, Uwe, Boal, Amie K., Bollinger, J. Martin, Krebs, Carsten, Högbom, Martin, Phillips, George N., Vierstra, Richard D., Sauter, Nicholas K., Orville, Allen M., Kern, Jan, Yachandra, Vittal K., and Yano, Junko. Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. United States: N. p., 2017. Web. doi:10.1038/nmeth.4195.
Copy to clipboard
Fuller, Franklin D., Gul, Sheraz, Chatterjee, Ruchira, Burgie, E. Sethe, Young, Iris D., Lebrette, Hugo, Srinivas, Vivek, Brewster, Aaron S., Michels-Clark, Tara, Clinger, Jonathan A., Andi, Babak, Ibrahim, Mohamed, Pastor, Ernest, de Lichtenberg, Casper, Hussein, Rana, Pollock, Christopher J., Zhang, Miao, Stan, Claudiu A., Kroll, Thomas, Fransson, Thomas, Weninger, Clemens, Kubin, Markus, Aller, Pierre, Lassalle, Louise, Bräuer, Philipp, Miller, Mitchell D., Amin, Muhamed, Koroidov, Sergey, Roessler, Christian G., Allaire, Marc, Sierra, Raymond G., Docker, Peter T., Glownia, James M., Nelson, Silke, Koglin, Jason E., Zhu, Diling, Chollet, Matthieu, Song, Sanghoon, Lemke, Henrik, Liang, Mengning, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Zouni, Athina, Messinger, Johannes, Bergmann, Uwe, Boal, Amie K., Bollinger, J. Martin, Krebs, Carsten, Högbom, Martin, Phillips, George N., Vierstra, Richard D., Sauter, Nicholas K., Orville, Allen M., Kern, Jan, Yachandra, Vittal K., & Yano, Junko. Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. United States. https://doi.org/10.1038/nmeth.4195
Copy to clipboard
Fuller, Franklin D., Gul, Sheraz, Chatterjee, Ruchira, Burgie, E. Sethe, Young, Iris D., Lebrette, Hugo, Srinivas, Vivek, Brewster, Aaron S., Michels-Clark, Tara, Clinger, Jonathan A., Andi, Babak, Ibrahim, Mohamed, Pastor, Ernest, de Lichtenberg, Casper, Hussein, Rana, Pollock, Christopher J., Zhang, Miao, Stan, Claudiu A., Kroll, Thomas, Fransson, Thomas, Weninger, Clemens, Kubin, Markus, Aller, Pierre, Lassalle, Louise, Bräuer, Philipp, Miller, Mitchell D., Amin, Muhamed, Koroidov, Sergey, Roessler, Christian G., Allaire, Marc, Sierra, Raymond G., Docker, Peter T., Glownia, James M., Nelson, Silke, Koglin, Jason E., Zhu, Diling, Chollet, Matthieu, Song, Sanghoon, Lemke, Henrik, Liang, Mengning, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Zouni, Athina, Messinger, Johannes, Bergmann, Uwe, Boal, Amie K., Bollinger, J. Martin, Krebs, Carsten, Högbom, Martin, Phillips, George N., Vierstra, Richard D., Sauter, Nicholas K., Orville, Allen M., Kern, Jan, Yachandra, Vittal K., and Yano, Junko. Mon . "Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers". United States. https://doi.org/10.1038/nmeth.4195. https://www.osti.gov/servlets/purl/1360909.
Copy to clipboard
@article{osti_1360909,
title = {Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers},
author = {Fuller, Franklin D. and Gul, Sheraz and Chatterjee, Ruchira and Burgie, E. Sethe and Young, Iris D. and Lebrette, Hugo and Srinivas, Vivek and Brewster, Aaron S. and Michels-Clark, Tara and Clinger, Jonathan A. and Andi, Babak and Ibrahim, Mohamed and Pastor, Ernest and de Lichtenberg, Casper and Hussein, Rana and Pollock, Christopher J. and Zhang, Miao and Stan, Claudiu A. and Kroll, Thomas and Fransson, Thomas and Weninger, Clemens and Kubin, Markus and Aller, Pierre and Lassalle, Louise and Bräuer, Philipp and Miller, Mitchell D. and Amin, Muhamed and Koroidov, Sergey and Roessler, Christian G. and Allaire, Marc and Sierra, Raymond G. and Docker, Peter T. and Glownia, James M. and Nelson, Silke and Koglin, Jason E. and Zhu, Diling and Chollet, Matthieu and Song, Sanghoon and Lemke, Henrik and Liang, Mengning and Sokaras, Dimosthenis and Alonso-Mori, Roberto and Zouni, Athina and Messinger, Johannes and Bergmann, Uwe and Boal, Amie K. and Bollinger, J. Martin and Krebs, Carsten and Högbom, Martin and Phillips, George N. and Vierstra, Richard D. and Sauter, Nicholas K. and Orville, Allen M. and Kern, Jan and Yachandra, Vittal K. and Yano, Junko},
abstractNote = {© 2017 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.},
doi = {10.1038/nmeth.4195},
journal = {Nature Methods},
number = 4,
volume = 14,
place = {United States},
year = {2017},
month = {2}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/nmeth.4195
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 14 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Kinetics of Electron Transfer from Q A to Q B in Photosystem II †
journal, October 2001

  • de Wijn, Rik; van Gorkom, Hans J.
  • Biochemistry, Vol. 40, Issue 39
  • DOI: 10.1021/bi010852r

High Resolution Structure of Deinococcus Bacteriophytochrome Yields New Insights into Phytochrome Architecture and Evolution
journal, February 2007

  • Wagner, Jeremiah R.; Zhang, Junrui; Brunzelle, Joseph S.
  • Journal of Biological Chemistry, Vol. 282, Issue 16
  • DOI: 10.1074/jbc.M611824200

Probing the 3d Spin Momentum with X-ray Emission Spectroscopy:  The Case of Molecular-Spin Transitions
journal, June 2006

  • Vankó, György; Neisius, Thomas; Molnár, Gábor
  • The Journal of Physical Chemistry B, Vol. 110, Issue 24
  • DOI: 10.1021/jp0615961

Origins of Fluorescence in Evolved Bacteriophytochromes
journal, September 2014

  • Bhattacharya, Shyamosree; Auldridge, Michele E.; Lehtivuori, Heli
  • Journal of Biological Chemistry, Vol. 289, Issue 46
  • DOI: 10.1074/jbc.M114.589739

Native-like Photosystem II Superstructure at 2.44 Å Resolution through Detergent Extraction from the Protein Crystal
journal, November 2014

Grease matrix as a versatile carrier of proteins for serial crystallography
journal, November 2014

  • Sugahara, Michihiro; Mizohata, Eiichi; Nango, Eriko
  • Nature Methods, Vol. 12, Issue 1
  • DOI: 10.1038/nmeth.3172

X-ray Radiation Induces Deprotonation of the Bilin Chromophore in Crystalline D. radiodurans Phytochrome
journal, February 2015

  • Li, Feifei; Burgie, E. Sethe; Yu, Tao
  • Journal of the American Chemical Society, Vol. 137, Issue 8
  • DOI: 10.1021/ja510923m

Time-resolved absorption studies of native etiolated oat phytochrome
journal, June 1992

  • Zhang, Chian Fan; Farrens, David L.; Bjorling, Sofie C.
  • Journal of the American Chemical Society, Vol. 114, Issue 12
  • DOI: 10.1021/ja00038a019

New Python-based methods for data processing
journal, June 2013

  • Sauter, Nicholas K.; Hattne, Johan; Grosse-Kunstleve, Ralf W.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
  • DOI: 10.1107/S0907444913000863

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
journal, May 2012

Femtosecond X-ray protein nanocrystallography
journal, February 2011

  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
  • Nature, Vol. 470, Issue 7332, p. 73-77
  • DOI: 10.1038/nature09750

High resolution 1s core hole X-ray spectroscopy in 3d transition metal complexes—electronic and structural information
journal, January 2005

Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy
journal, July 2014

  • Kern, Jan; Tran, Rosalie; Alonso-Mori, Roberto
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms5371

Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography
journal, July 2016

  • Oghbaey, Saeed; Sarracini, Antoine; Ginn, Helen M.
  • Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 8, p. 944-955
  • DOI: 10.1107/S2059798316010834

A Manganese(IV)/Iron(III) Cofactor in Chlamydia trachomatis Ribonucleotide Reductase
journal, May 2007

Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature
journal, February 2013

The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
journal, October 2016

  • Edlund, Petra; Takala, Heikki; Claesson, Elin
  • Scientific Reports, Vol. 6, Article No. 35279
  • DOI: 10.1038/srep35279

Towards characterization of photo-excited electron transfer and catalysis in natural and artificial systems using XFELs
journal, January 2016

  • Alonso-Mori, R.; Asa, K.; Bergmann, U.
  • Faraday Discussions, Vol. 194
  • DOI: 10.1039/C6FD00084C

The Chromophore Structural Changes during the Photocycle of Phytochrome:  A Combined Resonance Raman and Quantum Chemical Approach
journal, April 2007

  • Mroginski, Maria Andrea; Murgida, Daniel H.; Hildebrandt, Peter
  • Accounts of Chemical Research, Vol. 40, Issue 4
  • DOI: 10.1021/ar6000523

Robust indexing for automatic data collection
journal, May 2004

  • Sauter, Nicholas K.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 37, Issue 3
  • DOI: 10.1107/S0021889804005874

Energy-dispersive X-ray emission spectroscopy using an X-ray free-electron laser in a shot-by-shot mode
journal, November 2012

  • Alonso-Mori, R.; Kern, J.; Gildea, R. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 47, p. 19103-19107
  • DOI: 10.1073/pnas.1211384109

Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules
journal, December 2015

  • Levantino, Matteo; Yorke, Briony A.; Monteiro, Diana CF
  • Current Opinion in Structural Biology, Vol. 35
  • DOI: 10.1016/j.sbi.2015.07.017

Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers
journal, March 2014

  • Hattne, Johan; Echols, Nathaniel; Tran, Rosalie
  • Nature Methods, Vol. 11, Issue 5
  • DOI: 10.1038/nmeth.2887

Purification, characterisation and crystallisation of photosystem II from Thermosynechococcus elongatus cultivated in a new type of photobioreactor
journal, January 2005

  • Kern, J.; Loll, B.; Lüneberg, C.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1706, Issue 1-2
  • DOI: 10.1016/j.bbabio.2004.10.007

A Perspective on Enzyme Catalysis
journal, August 2003

Structure of photosystem II and substrate binding at room temperature
journal, November 2016

  • Young, Iris D.; Ibrahim, Mohamed; Chatterjee, Ruchira
  • Nature, Vol. 540, Issue 7633
  • DOI: 10.1038/nature20161

First lasing and operation of an ångstrom-wavelength free-electron laser
journal, August 2010

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
journal, February 2014

  • Weierstall, Uwe; James, Daniel; Wang, Chong
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4309

Mn 4 Ca Cluster in Photosynthesis: Where and How Water is Oxidized to Dioxygen
journal, March 2014

  • Yano, Junko; Yachandra, Vittal
  • Chemical Reviews, Vol. 114, Issue 8
  • DOI: 10.1021/cr4004874

MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

  • Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
  • DOI: 10.1107/S0907444909042073

Coot model-building tools for molecular graphics
journal, November 2004

  • Emsley, Paul; Cowtan, Kevin
  • Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
  • DOI: 10.1107/S0907444904019158

Fixed-target protein serial microcrystallography with an x-ray free electron laser
journal, August 2014

  • Hunter, Mark S.; Segelke, Brent; Messerschmidt, Marc
  • Scientific Reports, Vol. 4, Issue 1
  • DOI: 10.1038/srep06026

Local moments and electronic correlations in Fe-based Heusler alloys: Kα x-ray emission spectra measurements
journal, September 2016

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
journal, August 2013

  • Roessler, Christian G.; Kuczewski, Anthony; Stearns, Richard
  • Journal of Synchrotron Radiation, Vol. 20, Issue 5
  • DOI: 10.1107/S0909049513020372

High-density grids for efficient data collection from multiple crystals
journal, January 2016

  • Baxter, Elizabeth L.; Aguila, Laura; Alonso-Mori, Roberto
  • Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 1, p. 2-11
  • DOI: 10.1107/S2059798315020847

A multi-crystal wavelength dispersive x-ray spectrometer
journal, July 2012

  • Alonso-Mori, Roberto; Kern, Jan; Sokaras, Dimosthenis
  • Review of Scientific Instruments, Vol. 83, Issue 7
  • DOI: 10.1063/1.4737630

CSPAD-140k: A versatile detector for LCLS experiments
journal, August 2013

  • Herrmann, Sven; Boutet, Sébastien; Duda, Brian
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 718
  • DOI: 10.1016/j.nima.2013.01.057

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
journal, May 2016

  • Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan
  • Journal of Applied Crystallography, Vol. 49, Issue 3
  • DOI: 10.1107/S1600576716006348

Phytochrome photoconversion
journal, June 1997

Divergent assembly mechanisms of the manganese/iron cofactors in R2lox and R2c proteins
journal, September 2016

Assembly of nonheme Mn/Fe active sites in heterodinuclear metalloproteins
journal, April 2014

  • Griese, Julia J.; Srinivas, Vivek; Högbom, Martin
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 19, Issue 6
  • DOI: 10.1007/s00775-014-1140-7

Photon-in photon-out hard X-ray spectroscopy at the Linac Coherent Light Source
journal, April 2015

  • Alonso-Mori, Roberto; Sokaras, Dimosthenis; Zhu, Diling
  • Journal of Synchrotron Radiation, Vol. 22, Issue 3
  • DOI: 10.1107/S1600577515004488

O 2 -Evolving Chlorite Dismutase as a Tool for Studying O 2 -Utilizing Enzymes
journal, February 2012

  • Dassama, Laura M. K.; Yosca, Timothy H.; Conner, Denise A.
  • Biochemistry, Vol. 51, Issue 8
  • DOI: 10.1021/bi201906x

The X-ray Pump–Probe instrument at the Linac Coherent Light Source
journal, April 2015

  • Chollet, Matthieu; Alonso-Mori, Roberto; Cammarata, Marco
  • Journal of Synchrotron Radiation, Vol. 22, Issue 3
  • DOI: 10.1107/S1600577515005135

COOPERATION OF CHARGES IN PHOTOSYNTHETIC O 2 EVOLUTION–I. A LINEAR FOUR STEP MECHANISM
journal, June 1970

Improvements in serial femtosecond crystallography of photosystem II by optimizing crystal uniformity using microseeding procedures
journal, July 2015

  • Ibrahim, Mohamed; Chatterjee, Ruchira; Hellmich, Julia
  • Structural Dynamics, Vol. 2, Issue 4
  • DOI: 10.1063/1.4919741

Crystallographic and Electron Microscopic Analyses of a Bacterial Phytochrome Reveal Local and Global Rearrangements during Photoconversion
journal, July 2014

  • Burgie, E. Sethe; Wang, Tong; Bussell, Adam N.
  • Journal of Biological Chemistry, Vol. 289, Issue 35
  • DOI: 10.1074/jbc.M114.571661

Gas dynamic virtual nozzle for generation of microscopic droplet streams
journal, September 2008

  • DePonte, D. P.; Weierstall, U.; Schmidt, K.
  • Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505
  • DOI: 10.1088/0022-3727/41/19/195505

Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography
journal, April 2016

Nanoflow electrospinning serial femtosecond crystallography
journal, October 2012

  • Sierra, Raymond G.; Laksmono, Hartawan; Kern, Jan
  • Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 11, p. 1584-1587
  • DOI: 10.1107/S0907444912038152

Droplet On Tape: Protocol
journal, February 2017

The Chromophore Structural Changes During the Photocycle of Phytochrome: A Combined Resonance Raman and Quantum Chemical Approach
journal, July 2007

  • Mroginski, Maria Andrea; Murgida, Daniel H.; Hildebrandt, Peter
  • ChemInform, Vol. 38, Issue 29
  • DOI: 10.1002/chin.200729277

Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen
journal, August 2020

  • Zacarias, Sónia; Temporão, Adriana; Carpentier, Philippe
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 25, Issue 6
  • DOI: 10.1007/s00775-020-01814-y

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
journal, February 2021

Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering
text, January 2016

  • Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan
  • Deutsches Elektronen-Synchrotron, DESY, Hamburg
  • DOI: 10.3204/pubdb-2016-02690

The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
text, January 2016

  • Edlund, Petra; Takala, Heikki; Claesson, Elin
  • Deutsches Elektronen-Synchrotron, DESY, Hamburg
  • DOI: 10.3204/pubdb-2016-04471

PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.45787
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    DIY XES-development of an inexpensive, versatile, and easy to fabricate XES analyzer and sample delivery system
    journal, February 2019

    • Jensen, Scott C.; Sullivan, Brendan; Hartzler, Daniel A.
    • X-Ray Spectrometry, Vol. 48, Issue 5
    • DOI: 10.1002/xrs.3005

    In Situ Electrochemical Cells to Study the Oxygen Evolution Reaction by Near Ambient Pressure X-ray Photoelectron Spectroscopy
    journal, October 2018

    Crystal-on-crystal chips for in situ serial diffraction at room temperature
    journal, January 2018

    • Ren, Zhong; Ayhan, Medine; Bandara, Sepalika
    • Lab on a Chip, Vol. 18, Issue 15
    • DOI: 10.1039/c8lc00489g

    Perspective: Opportunities for ultrafast science at SwissFEL
    journal, November 2017

    • Abela, Rafael; Beaud, Paul; van Bokhoven, Jeroen A.
    • Structural Dynamics, Vol. 4, Issue 6
    • DOI: 10.1063/1.4997222

    Rapid mixing of colliding picoliter liquid droplets delivered through-space from piezoelectric-actuated pipettes characterized by time-resolved fluorescence monitoring
    journal, May 2019

    • Tellkamp, Friedjof; Khajehpour, Mazdak; Robertson, Wesley D.
    • Review of Scientific Instruments, Vol. 90, Issue 5
    • DOI: 10.1063/1.5050270

    On-demand droplet loading of ultrasonic acoustic levitator and its application for protein crystallography experiments
    journal, May 2019

    • Tsujino, Soichiro; Shinoda, Akira; Tomizaki, Takashi
    • Applied Physics Letters, Vol. 114, Issue 21
    • DOI: 10.1063/1.5095574

    Photoreversible interconversion of a phytochrome photosensory module in the crystalline state
    journal, December 2019

    • Burgie, E. Sethe; Clinger, Jonathan A.; Miller, Mitchell D.
    • Proceedings of the National Academy of Sciences, Vol. 117, Issue 1
    • DOI: 10.1073/pnas.1912041116

    An outlook on using serial femtosecond crystallography in drug discovery
    journal, May 2019

    Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography
    journal, November 2017

    • Schulz, Eike C.; Kaub, Johannes; Busse, Frederik
    • Journal of Applied Crystallography, Vol. 50, Issue 6
    • DOI: 10.1107/s1600576717014479

    A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies
    journal, March 2019

    • Sharma, Amit; Berntsen, Peter; Harimoorthy, Rajiv
    • Journal of Applied Crystallography, Vol. 52, Issue 2
    • DOI: 10.1107/s1600576719001900

    3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating
    journal, August 2019

    • Echelmeier, Austin; Kim, Daihyun; Cruz Villarreal, Jorvani
    • Journal of Applied Crystallography, Vol. 52, Issue 5
    • DOI: 10.1107/s1600576719010343

    Successful sample preparation for serial crystallography experiments
    journal, November 2019

    • Beale, John H.; Bolton, Rachel; Marshall, Stephen A.
    • Journal of Applied Crystallography, Vol. 52, Issue 6
    • DOI: 10.1107/s1600576719013517

    A convolutional neural network-based screening tool for X-ray serial crystallography
    journal, April 2018

    • Ke, Tsung-Wei; Brewster, Aaron S.; Yu, Stella X.
    • Journal of Synchrotron Radiation, Vol. 25, Issue 3, p. 655-670
    • DOI: 10.1107/s1600577518004873

    The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source
    journal, February 2019

    • Sierra, Raymond G.; Batyuk, Alexander; Sun, Zhibin
    • Journal of Synchrotron Radiation, Vol. 26, Issue 2
    • DOI: 10.1107/s1600577519001577

    XANES and EXAFS of dilute solutions of transition metals at XFELs
    journal, August 2019

    • Chatterjee, Ruchira; Weninger, Clemens; Loukianov, Anton
    • Journal of Synchrotron Radiation, Vol. 26, Issue 5
    • DOI: 10.1107/s1600577519007550

    Reducing sample consumption for serial crystallography using acoustic drop ejection
    journal, August 2019

    • Davy, Bradley; Axford, Danny; Beale, John H.
    • Journal of Synchrotron Radiation, Vol. 26, Issue 5
    • DOI: 10.1107/s1600577519009329

    XFELs for structure and dynamics in biology
    journal, May 2017

    Solving protein structure from sparse serial microcrystal diffraction data at a storage-ring synchrotron source
    journal, July 2018

    A fixed-target platform for serial femtosecond crystallography in a hydrated environment
    journal, January 2020

    Where is crystallography going?
    journal, February 2018

    • Grimes, Jonathan M.; Hall, David R.; Ashton, Alun W.
    • Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 2
    • DOI: 10.1107/s2059798317016709

    Using sound pulses to solve the crystal-harvesting bottleneck
    journal, October 2018

    • Samara, Yasmin N.; Brennan, Haley M.; McCarthy, Liam
    • Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10
    • DOI: 10.1107/s2059798318011506

    Crystallography on a chip – without the chip: sheet-on-sheet sandwich
    journal, October 2018

    • Doak, R. Bruce; Nass Kovacs, Gabriela; Gorel, Alexander
    • Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10
    • DOI: 10.1107/s2059798318011634

    Sample delivery for serial crystallography at free-electron lasers and synchrotrons
    journal, January 2019

    • Grünbein, Marie Luise; Nass Kovacs, Gabriela
    • Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
    • DOI: 10.1107/s205979831801567x

    Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography
    journal, January 2019

    Strategies for sample delivery for femtosecond crystallography
    journal, February 2019

    • Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E.
    • Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
    • DOI: 10.1107/s2059798318017953

    A guide to sample delivery systems for serial crystallography
    journal, August 2019

    • Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai
    • The FEBS Journal, Vol. 286, Issue 22
    • DOI: 10.1111/febs.15099

    Structural isomers of the S 2 state in photosystem II: do they exist at room temperature and are they important for function?
    journal, March 2019

    • Chatterjee, Ruchira; Lassalle, Louise; Gul, Sheraz
    • Physiologia Plantarum, Vol. 166, Issue 1
    • DOI: 10.1111/ppl.12947

    Acoustophoretic printing
    journal, August 2018

    • Foresti, Daniele; Kroll, Katharina T.; Amissah, Robert
    • Science Advances, Vol. 4, Issue 8
    • DOI: 10.1126/sciadv.aat1659

    Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies
    journal, December 2019

    • Nango, Eriko; Kubo, Minoru; Tono, Kensuke
    • Applied Sciences, Vol. 9, Issue 24
    • DOI: 10.3390/app9245505

    Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate
    journal, November 2017

    • Tosha, Takehiko; Nomura, Takashi; Nishida, Takuma
    • Nature Communications, Vol. 8, Article No. 1585
    • DOI: 10.1038/s41467-017-01702-1

    Structures of the intermediates of Kok’s photosynthetic water oxidation clock
    journal, November 2018

    Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation
    journal, August 2019

    On-demand sample injection: combining acoustic actuation with a tear-drop shaped nozzle to generate droplets with precise spatial and temporal control
    journal, January 2020

    • Brenker, Jason C.; Devendran, Citsabehsan; Neild, Adrian
    • Lab on a Chip, Vol. 20, Issue 2
    • DOI: 10.1039/c9lc00837c

    A fixed-target platform for serial femtosecond crystallography in a hydrated environment
    text, January 2020

    • Shelby, M. L.; Gilbile, D.; Grant, T. D.
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-05316

    Acoustophoretic printing
    text, January 2018

    Perspective: Opportunities for ultrafast science at SwissFEL
    text, January 2017

    Strategies for sample delivery for femtosecond crystallography
    text, January 2019

    Perspective: Opportunities for ultrafast science at SwissFEL
    text, January 2017

      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: