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Title: Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass

Abstract

This study explored integrated conversion of corn stover to ethanol and highlights techniques for accurate yield calculations. Acid pretreated corn stover (PCS) produced in a pilot-scale reactor was enzymatically hydrolyzed and the resulting sugars were fermented to ethanol by the glucose–xylose fermenting bacteria, Zymomonas mobilis 8b. The calculations account for high solids operation and oligomeric sugars produced during pretreatment, enzymatic hydrolysis, and fermentation, which, if not accounted for, leads to overestimating ethanol yields. The calculations are illustrated for enzymatic hydrolysis and fermentation of PCS at 17.5% and 20.0% total solids achieving 80.1% and 77.9% conversion of cellulose and xylan to ethanol and ethanol titers of 63 g/L and 69 g/L, respectively. In the future, these techniques, including the TEA results, will be applied to fully integrated pilot-scale runs.

Authors:
 [1];  [1];  [2];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
  2. P2 Science, Woodbridge, CT (United States)
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
OSTI Identifier:
1237439
Alternate Identifier(s):
OSTI ID: 1358840
Report Number(s):
NREL/JA-5100-65494
Journal ID: ISSN 0960-8524
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 205; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; pretreatment; enzymatic hydrolysis; fermentation; ethanol; corn stover; yields; mass balance

Citation Formats

Schell, Daniel J., Dowe, Nancy, Chapeaux, Alexandre, Nelson, Robert S., and Jennings, Edward W. Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass. United States: N. p., 2016. Web. doi:10.1016/j.biortech.2016.01.024.
Schell, Daniel J., Dowe, Nancy, Chapeaux, Alexandre, Nelson, Robert S., & Jennings, Edward W. Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass. United States. https://doi.org/10.1016/j.biortech.2016.01.024
Schell, Daniel J., Dowe, Nancy, Chapeaux, Alexandre, Nelson, Robert S., and Jennings, Edward W. Tue . "Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass". United States. https://doi.org/10.1016/j.biortech.2016.01.024. https://www.osti.gov/servlets/purl/1237439.
@article{osti_1237439,
title = {Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass},
author = {Schell, Daniel J. and Dowe, Nancy and Chapeaux, Alexandre and Nelson, Robert S. and Jennings, Edward W.},
abstractNote = {This study explored integrated conversion of corn stover to ethanol and highlights techniques for accurate yield calculations. Acid pretreated corn stover (PCS) produced in a pilot-scale reactor was enzymatically hydrolyzed and the resulting sugars were fermented to ethanol by the glucose–xylose fermenting bacteria, Zymomonas mobilis 8b. The calculations account for high solids operation and oligomeric sugars produced during pretreatment, enzymatic hydrolysis, and fermentation, which, if not accounted for, leads to overestimating ethanol yields. The calculations are illustrated for enzymatic hydrolysis and fermentation of PCS at 17.5% and 20.0% total solids achieving 80.1% and 77.9% conversion of cellulose and xylan to ethanol and ethanol titers of 63 g/L and 69 g/L, respectively. In the future, these techniques, including the TEA results, will be applied to fully integrated pilot-scale runs.},
doi = {10.1016/j.biortech.2016.01.024},
journal = {Bioresource Technology},
number = C,
volume = 205,
place = {United States},
year = {Tue Jan 19 00:00:00 EST 2016},
month = {Tue Jan 19 00:00:00 EST 2016}
}

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Cited by: 14 works
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Works referenced in this record:

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Works referencing / citing this record:

Enzyme production and oil palm empty fruit bunch bioconversion to ethanol using a hybrid yeast strain
journal, September 2019

  • Zhai, Lili; Manglekar, Rupali Rahul; Geng, Anli
  • Biotechnology and Applied Biochemistry, Vol. 67, Issue 5
  • DOI: 10.1002/bab.1816

Zymomonas mobilis as a model system for production of biofuels and biochemicals
journal, September 2016


Ternary Blended Chitosan/Chitin/ $$\hbox {FE}_{3}\hbox {O}_{4}$$ FE 3 O 4 Nanosupport for Lipase Activation and Stabilization
journal, February 2019

  • Rahman, Ida Nurhazwani Abdul; Wahab, Roswanira Abdul; Mahat, Naji Arafat
  • Arabian Journal for Science and Engineering, Vol. 44, Issue 7
  • DOI: 10.1007/s13369-019-03771-4

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis
journal, February 2018


Zymomonas mobilis as a model system for production of biofuels and biochemicals
journal, September 2016