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Title: Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes

Modern microbial mats provide remarkable insights into assembly, function and origin of complex microbial ecosystems. An excellent model of such systems is located in Shark Bay, Australia. Although bacteria have been extensively investigated in these communities, the role of archaea in microbial mats is poorly understood. Delineating the spatial distribution of archaea with mat depth will enable resolution of specific niches associated with this domain. In the present study, high throughput amplicon sequencing was undertaken in conjunction with key biogeochemical properties of two mat types (smooth and pustular). A total of 13,547,552 unfiltered sequences were obtained, and classified sequences were affiliated to three archaeal and candidate phyla, Parvarchaeota, Euryarchaeota and Crenarchaeota. One way analysis of similarity tests (ANOSIM) indicated the archaeal community structures of smooth and pustular mats were significantly different (global R = 1, p = 0.1 %). Smooth mats possessed higher archaeal diversity, dominated by Parvarchaeota, followed by Thermoplasmata, Class marine benthic group B and Halobacteria. The methanogenic community in smooth mats was dominated by hydrogenotrophic Methanomicrobiales, as well as methylotrophic Methanosarcinales, Methanococcales, Methanobacteriales and Methanomassiliicoccaceae. Conversely, pustular mats were enriched with Halobacteria and Parvarchaeota. The rates of oxygen production/consumption as well as sulphate reduction were up tomore » four times higher in smooth than in pustular mats. Methane production peaked in the oxic part of mats and was up to seven-fold higher in smooth than in pustular mats. Metabolic cooperation in putative surface anoxic niches is proposed to be key in efficient cycling of key nutrients in these systems.« less
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [1]
  1. The Univ. of New South Wales, Sydney (Australia)
  2. Univ. of Connecticut, Storrs, CT (United States); Univ. of New South Wales, Sydney (Australia)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Univ. of Connecticut, Storrs, CT (United States)
Publication Date:
Report Number(s):
PNNL-SA-120053
Journal ID: ISSN 2045-2322
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; archaea; microbial ecology
OSTI Identifier:
1358501

Wong, Hon Lun, Visscher, Pieter T., White, III, Richard Allen, Smith, Daniela -Lee, Patterson, Molly M., and Burns, Brendan P.. Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes. United States: N. p., Web. doi:10.1038/srep46160.
Wong, Hon Lun, Visscher, Pieter T., White, III, Richard Allen, Smith, Daniela -Lee, Patterson, Molly M., & Burns, Brendan P.. Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes. United States. doi:10.1038/srep46160.
Wong, Hon Lun, Visscher, Pieter T., White, III, Richard Allen, Smith, Daniela -Lee, Patterson, Molly M., and Burns, Brendan P.. 2017. "Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes". United States. doi:10.1038/srep46160. https://www.osti.gov/servlets/purl/1358501.
@article{osti_1358501,
title = {Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes},
author = {Wong, Hon Lun and Visscher, Pieter T. and White, III, Richard Allen and Smith, Daniela -Lee and Patterson, Molly M. and Burns, Brendan P.},
abstractNote = {Modern microbial mats provide remarkable insights into assembly, function and origin of complex microbial ecosystems. An excellent model of such systems is located in Shark Bay, Australia. Although bacteria have been extensively investigated in these communities, the role of archaea in microbial mats is poorly understood. Delineating the spatial distribution of archaea with mat depth will enable resolution of specific niches associated with this domain. In the present study, high throughput amplicon sequencing was undertaken in conjunction with key biogeochemical properties of two mat types (smooth and pustular). A total of 13,547,552 unfiltered sequences were obtained, and classified sequences were affiliated to three archaeal and candidate phyla, Parvarchaeota, Euryarchaeota and Crenarchaeota. One way analysis of similarity tests (ANOSIM) indicated the archaeal community structures of smooth and pustular mats were significantly different (global R = 1, p = 0.1 %). Smooth mats possessed higher archaeal diversity, dominated by Parvarchaeota, followed by Thermoplasmata, Class marine benthic group B and Halobacteria. The methanogenic community in smooth mats was dominated by hydrogenotrophic Methanomicrobiales, as well as methylotrophic Methanosarcinales, Methanococcales, Methanobacteriales and Methanomassiliicoccaceae. Conversely, pustular mats were enriched with Halobacteria and Parvarchaeota. The rates of oxygen production/consumption as well as sulphate reduction were up to four times higher in smooth than in pustular mats. Methane production peaked in the oxic part of mats and was up to seven-fold higher in smooth than in pustular mats. Metabolic cooperation in putative surface anoxic niches is proposed to be key in efficient cycling of key nutrients in these systems.},
doi = {10.1038/srep46160},
journal = {Scientific Reports},
number = ,
volume = 7,
place = {United States},
year = {2017},
month = {4}
}