Metabolic engineering strategies for microbial synthesis of oleochemicals
Abstract
Microbial synthesis of oleochemicals has advanced significantly in the last decade. Microbes have been engineered to convert renewable substrates to a wide range of molecules that are ordinarily made from plant oils. This approach is attractive because it can reduce a motivation for converting tropical rainforest into farmland while simultaneously enabling access to molecules that are currently expensive to produce from oil crops. In the last decade, enzymes responsible for producing oleochemicals in nature have been identified, strategies to circumvent native regulation have been developed, and high yielding strains have been designed, built, and successfully demonstrated. Furthermore, this review will describe the metabolic pathways that lead to the diverse molecular features found in natural oleochemicals, highlight successful metabolic engineering strategies, and comment on areas where future work could further advance the field.
- Authors:
-
- University of Wisconsin-Madison, WI (United States)
- Chalmers University of Technology, Göteborg (Sweden)
- Publication Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); University of Wisconsin-Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 2274735
- Alternate Identifier(s):
- OSTI ID: 1357837; OSTI ID: 2279199
- Grant/Contract Number:
- SC0008744
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Metabolic Engineering
- Additional Journal Information:
- Journal Volume: 29; Journal ID: ISSN 1096-7176
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Oleochemical; Fatty acid; Metabolic engineering; Synthetic biology; Biodisesel
Citation Formats
Pfleger, Brian F., Gossing, Michael, and Nielsen, Jens. Metabolic engineering strategies for microbial synthesis of oleochemicals. United States: N. p., 2015.
Web. doi:10.1016/j.ymben.2015.01.009.
Pfleger, Brian F., Gossing, Michael, & Nielsen, Jens. Metabolic engineering strategies for microbial synthesis of oleochemicals. United States. https://doi.org/10.1016/j.ymben.2015.01.009
Pfleger, Brian F., Gossing, Michael, and Nielsen, Jens. Sat .
"Metabolic engineering strategies for microbial synthesis of oleochemicals". United States. https://doi.org/10.1016/j.ymben.2015.01.009. https://www.osti.gov/servlets/purl/2274735.
@article{osti_2274735,
title = {Metabolic engineering strategies for microbial synthesis of oleochemicals},
author = {Pfleger, Brian F. and Gossing, Michael and Nielsen, Jens},
abstractNote = {Microbial synthesis of oleochemicals has advanced significantly in the last decade. Microbes have been engineered to convert renewable substrates to a wide range of molecules that are ordinarily made from plant oils. This approach is attractive because it can reduce a motivation for converting tropical rainforest into farmland while simultaneously enabling access to molecules that are currently expensive to produce from oil crops. In the last decade, enzymes responsible for producing oleochemicals in nature have been identified, strategies to circumvent native regulation have been developed, and high yielding strains have been designed, built, and successfully demonstrated. Furthermore, this review will describe the metabolic pathways that lead to the diverse molecular features found in natural oleochemicals, highlight successful metabolic engineering strategies, and comment on areas where future work could further advance the field.},
doi = {10.1016/j.ymben.2015.01.009},
journal = {Metabolic Engineering},
number = ,
volume = 29,
place = {United States},
year = {Sat Feb 07 00:00:00 EST 2015},
month = {Sat Feb 07 00:00:00 EST 2015}
}
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