Efficient Cisplatin Pro-Drug Delivery Visualized with Sub-100 nm Resolution: Interfacing Engineered Thermosensitive Magnetomicelles with a Living System
Abstract
Temperature-responsive magnetic nanomicelles can serve as thermal energy and cargo carriers with controlled drug release functionality. In view of their potential biomedical applications, understanding the modes of interaction between nanomaterials and living systems and evaluation of efficiency of cargo delivery is of the utmost importance. In this paper, we investigate the interaction between the hybrid magnetic nanomicelles engineered for controlled platinum complex drug delivery and a biological system at three fundamental levels: subcellular compartments, a single cell and whole living animal. Nanomicelles with polymeric P(NIPAAm-co-AAm)-b-PCL core-shell were loaded with a hydrophobic Pt(IV) complex and Fe3O4 nanoparticles though self-assembly. The distribution of a platinum complex on subcellular level is visualized using hard X-ray fluorescence microscopy with unprecedented level of detail at sub-100 nm spatial resolution. We then study the cytotoxic effects of platinum complex-loaded micelles in vitro on a head and neck cancer cell culture model SQ20B. In conclusion, by employing the magnetic functionality of the micelles and additionally loading them with a near infrared fluorescent dye, we magnetically target them to a tumor site in a live animal xenografted model which allows to visualize their biodistribution in vivo.
- Authors:
-
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials. Materials Science Division
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials. Advanced Photon Source
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
- Univ. of Chicago, IL (United States). Dept. of Medicine
- Univ. of Chicago, IL (United States). Dept. of Radiology
- Argonne National Lab. (ANL), Argonne, IL (United States). Materials Science Division
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- Contributing Org.:
- Univ. of Chicago, IL (United States)
- OSTI Identifier:
- 1357470
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Advanced Materials Interfaces
- Additional Journal Information:
- Journal Volume: 1; Journal Issue: 7; Journal ID: ISSN 2196-7350
- Publisher:
- Wiley-VCH
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; nano-bio interfaces; thermosensitive magnetomicelles; drug delivery; hard x-ray fluorescence nanoprobe; sub-100 nm resolution
Citation Formats
Vitol, Elina A., Rozhkova, Elena A., Rose, Volker, Stripe, Benjamin D., Young, Natalie R., Cohen, Ezra E. W., Leoni, Lara, and Novosad, Valentyn. Efficient Cisplatin Pro-Drug Delivery Visualized with Sub-100 nm Resolution: Interfacing Engineered Thermosensitive Magnetomicelles with a Living System. United States: N. p., 2014.
Web. doi:10.1002/admi.201400182.
Vitol, Elina A., Rozhkova, Elena A., Rose, Volker, Stripe, Benjamin D., Young, Natalie R., Cohen, Ezra E. W., Leoni, Lara, & Novosad, Valentyn. Efficient Cisplatin Pro-Drug Delivery Visualized with Sub-100 nm Resolution: Interfacing Engineered Thermosensitive Magnetomicelles with a Living System. United States. https://doi.org/10.1002/admi.201400182
Vitol, Elina A., Rozhkova, Elena A., Rose, Volker, Stripe, Benjamin D., Young, Natalie R., Cohen, Ezra E. W., Leoni, Lara, and Novosad, Valentyn. Fri .
"Efficient Cisplatin Pro-Drug Delivery Visualized with Sub-100 nm Resolution: Interfacing Engineered Thermosensitive Magnetomicelles with a Living System". United States. https://doi.org/10.1002/admi.201400182. https://www.osti.gov/servlets/purl/1357470.
@article{osti_1357470,
title = {Efficient Cisplatin Pro-Drug Delivery Visualized with Sub-100 nm Resolution: Interfacing Engineered Thermosensitive Magnetomicelles with a Living System},
author = {Vitol, Elina A. and Rozhkova, Elena A. and Rose, Volker and Stripe, Benjamin D. and Young, Natalie R. and Cohen, Ezra E. W. and Leoni, Lara and Novosad, Valentyn},
abstractNote = {Temperature-responsive magnetic nanomicelles can serve as thermal energy and cargo carriers with controlled drug release functionality. In view of their potential biomedical applications, understanding the modes of interaction between nanomaterials and living systems and evaluation of efficiency of cargo delivery is of the utmost importance. In this paper, we investigate the interaction between the hybrid magnetic nanomicelles engineered for controlled platinum complex drug delivery and a biological system at three fundamental levels: subcellular compartments, a single cell and whole living animal. Nanomicelles with polymeric P(NIPAAm-co-AAm)-b-PCL core-shell were loaded with a hydrophobic Pt(IV) complex and Fe3O4 nanoparticles though self-assembly. The distribution of a platinum complex on subcellular level is visualized using hard X-ray fluorescence microscopy with unprecedented level of detail at sub-100 nm spatial resolution. We then study the cytotoxic effects of platinum complex-loaded micelles in vitro on a head and neck cancer cell culture model SQ20B. In conclusion, by employing the magnetic functionality of the micelles and additionally loading them with a near infrared fluorescent dye, we magnetically target them to a tumor site in a live animal xenografted model which allows to visualize their biodistribution in vivo.},
doi = {10.1002/admi.201400182},
journal = {Advanced Materials Interfaces},
number = 7,
volume = 1,
place = {United States},
year = {Fri Jun 06 00:00:00 EDT 2014},
month = {Fri Jun 06 00:00:00 EDT 2014}
}
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