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Title: Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis

Eukaryotic cells routinely compartmentalize metabolic pathways to particular organelles for biosynthetic purposes. Relatively few studies have addressed the cellular localization of pathways for secondary metabolites synthesis. In this study, the phytopathogenic fungus Fusarium graminearum reorganized its endoplasmic reticulum (ER) when triggered to produce mycotoxins, both in vitro and in planta. Fluorescence tagged biosynthetic proteins were found to co-localize with the modified ER as confirmed by co-fluorescence and co-purification with known ER proteins. Microscopy, cell sorting, and proteomics were applied in this FICUS collaborative effort.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [4] ;  [4] ;  [3] ;  [5]
  1. Univ. of Minnesota, St. Paul, MN (United States)
  2. USDA ARS Cereal Disease Lab., St. Paul, MN (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Oregon State Univ., Corvallis, OR (United States)
  5. USDA ARS Cereal Disease Lab., St. Paul, MN (United States); Univ. of Minnesota, St. Paul, MN (United States)
Publication Date:
Report Number(s):
PNNL-SA-125650
Journal ID: ISSN 2045-2322; KP1704020
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; endoplasmic reticulum; fungi; trichothecenes; fluorescence; proteomics; differentiation; fungal biology
OSTI Identifier:
1356473