Structure and specificity of a permissive bacterial C-prenyltransferase
Abstract
This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. In this work, PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug.
- Authors:
-
- Univ. of Kentucky, Lexington, KY (United States)
- Rice Univ., Houston, TX (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- National Institutes of Health (NIH); National Center for Advancing Translational Sciences (NCATS); Merck; USDOE Office of Science (SC); Eli Lilly and Company
- OSTI Identifier:
- 1356427
- Grant/Contract Number:
- R37 AI52188; R01 CA203257; U01 GM098248; UL1TR001998; AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Chemical Biology
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 4; Journal ID: ISSN 1552-4450
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biocatalysis; Biosynthesis; Enzymes; X-ray crystallography
Citation Formats
Elshahawi, Sherif I., Cao, Hongnan, Shaaban, Khaled A., Ponomareva, Larissa V., Subramanian, Thangaiah, Farman, Mark L., Spielmann, H. Peter, Phillips Jr., George N., Thorson, Jon S., and Singh, Shanteri. Structure and specificity of a permissive bacterial C-prenyltransferase. United States: N. p., 2017.
Web. doi:10.1038/nchembio.2285.
Elshahawi, Sherif I., Cao, Hongnan, Shaaban, Khaled A., Ponomareva, Larissa V., Subramanian, Thangaiah, Farman, Mark L., Spielmann, H. Peter, Phillips Jr., George N., Thorson, Jon S., & Singh, Shanteri. Structure and specificity of a permissive bacterial C-prenyltransferase. United States. https://doi.org/10.1038/nchembio.2285
Elshahawi, Sherif I., Cao, Hongnan, Shaaban, Khaled A., Ponomareva, Larissa V., Subramanian, Thangaiah, Farman, Mark L., Spielmann, H. Peter, Phillips Jr., George N., Thorson, Jon S., and Singh, Shanteri. Mon .
"Structure and specificity of a permissive bacterial C-prenyltransferase". United States. https://doi.org/10.1038/nchembio.2285. https://www.osti.gov/servlets/purl/1356427.
@article{osti_1356427,
title = {Structure and specificity of a permissive bacterial C-prenyltransferase},
author = {Elshahawi, Sherif I. and Cao, Hongnan and Shaaban, Khaled A. and Ponomareva, Larissa V. and Subramanian, Thangaiah and Farman, Mark L. and Spielmann, H. Peter and Phillips Jr., George N. and Thorson, Jon S. and Singh, Shanteri},
abstractNote = {This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. In this work, PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug.},
doi = {10.1038/nchembio.2285},
journal = {Nature Chemical Biology},
number = 4,
volume = 13,
place = {United States},
year = {Mon Feb 06 00:00:00 EST 2017},
month = {Mon Feb 06 00:00:00 EST 2017}
}
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