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Title: Light-sheet microscopy by confocal line scanning of dual-Bessel beams

Here, we have developed a light-sheet microscope that uses confocal scanning of dual-Bessel beams for illumination. A digital micromirror device (DMD) is placed in the intermediate image plane of the objective used to collect fluorescence and is programmed with two lines of pixels in the “on” state such that the DMD functions as a spatial filter to reject the out-of-focus background generated by the side-lobes of the Bessel beams. The optical sectioning and out-of-focus background rejection capabilities of this microscope were demonstrated by imaging of fluorescently stained actin in human A431 cells. The dual-Bessel beam system enables twice as many photons to be detected per imaging scan, which is useful for low light applications (e.g., single-molecule localization) or imaging at high speed with a superior signal to noise. While demonstrated for two Bessel beams, this approach is scalable to a larger number of beams.
 [1] ;  [2] ; ORCiD logo [2] ; ORCiD logo [2]
  1. Washington Univ., St. Louis, MO (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Report Number(s):
Journal ID: ISSN 1083-3688
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Journal of Biomedical Optics
Additional Journal Information:
Journal Volume: 21; Journal Issue: 10; Journal ID: ISSN 1083-3688
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE Laboratory Directed Research and Development (LDRD) Program
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; biological science; light sheet microscopy; fluorescence microscopy; three-dimensional microscopy; confocal microscopy
OSTI Identifier: