Light-sheet microscopy by confocal line scanning of dual-Bessel beams
Abstract
Here, we have developed a light-sheet microscope that uses confocal scanning of dual-Bessel beams for illumination. A digital micromirror device (DMD) is placed in the intermediate image plane of the objective used to collect fluorescence and is programmed with two lines of pixels in the “on” state such that the DMD functions as a spatial filter to reject the out-of-focus background generated by the side-lobes of the Bessel beams. The optical sectioning and out-of-focus background rejection capabilities of this microscope were demonstrated by imaging of fluorescently stained actin in human A431 cells. The dual-Bessel beam system enables twice as many photons to be detected per imaging scan, which is useful for low light applications (e.g., single-molecule localization) or imaging at high speed with a superior signal to noise. While demonstrated for two Bessel beams, this approach is scalable to a larger number of beams.
- Authors:
-
[2];
[2]
- Washington Univ., St. Louis, MO (United States)
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1356125
- Report Number(s):
- LA-UR-15-24283
Journal ID: ISSN 1083-3688
- Grant/Contract Number:
- AC52-06NA25396
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biomedical Optics
- Additional Journal Information:
- Journal Volume: 21; Journal Issue: 10; Journal ID: ISSN 1083-3688
- Publisher:
- SPIE
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; biological science; light sheet microscopy; fluorescence microscopy; three-dimensional microscopy; confocal microscopy
Citation Formats
Zhang, Pengfei, Phipps, Mary Elizabeth, Goodwin, Peter Marvin, and Werner, James H. Light-sheet microscopy by confocal line scanning of dual-Bessel beams. United States: N. p., 2016.
Web. doi:10.1117/1.JBO.21.10.100502.
Zhang, Pengfei, Phipps, Mary Elizabeth, Goodwin, Peter Marvin, & Werner, James H. Light-sheet microscopy by confocal line scanning of dual-Bessel beams. United States. https://doi.org/10.1117/1.JBO.21.10.100502
Zhang, Pengfei, Phipps, Mary Elizabeth, Goodwin, Peter Marvin, and Werner, James H. Tue .
"Light-sheet microscopy by confocal line scanning of dual-Bessel beams". United States. https://doi.org/10.1117/1.JBO.21.10.100502. https://www.osti.gov/servlets/purl/1356125.
@article{osti_1356125,
title = {Light-sheet microscopy by confocal line scanning of dual-Bessel beams},
author = {Zhang, Pengfei and Phipps, Mary Elizabeth and Goodwin, Peter Marvin and Werner, James H.},
abstractNote = {Here, we have developed a light-sheet microscope that uses confocal scanning of dual-Bessel beams for illumination. A digital micromirror device (DMD) is placed in the intermediate image plane of the objective used to collect fluorescence and is programmed with two lines of pixels in the “on” state such that the DMD functions as a spatial filter to reject the out-of-focus background generated by the side-lobes of the Bessel beams. The optical sectioning and out-of-focus background rejection capabilities of this microscope were demonstrated by imaging of fluorescently stained actin in human A431 cells. The dual-Bessel beam system enables twice as many photons to be detected per imaging scan, which is useful for low light applications (e.g., single-molecule localization) or imaging at high speed with a superior signal to noise. While demonstrated for two Bessel beams, this approach is scalable to a larger number of beams.},
doi = {10.1117/1.JBO.21.10.100502},
journal = {Journal of Biomedical Optics},
number = 10,
volume = 21,
place = {United States},
year = {Tue Oct 25 00:00:00 EDT 2016},
month = {Tue Oct 25 00:00:00 EDT 2016}
}
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Works referenced in this record:
Spinning-disk Confocal Microscopy — A Cutting-Edge Tool for Imaging of Membrane Traffic
journal, January 2002
- Nakano, Akihiko
- Cell Structure and Function, Vol. 27, Issue 5
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
journal, August 2004
- Huisken, J.; Swoger, J.; Del Bene, F.
- Science, Vol. 305, Issue 5686, p. 1007-1009
Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation
journal, January 2013
- Fahrbach, Florian O.; Gurchenkov, Vasily; Alessandri, Kevin
- Optics Express, Vol. 21, Issue 11
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
journal, March 2011
- Planchon, Thomas A.; Gao, Liang; Milkie, Daniel E.
- Nature Methods, Vol. 8, Issue 5
Light Sheet Microscopy for Single Molecule Tracking in Living Tissue
journal, July 2010
- Ritter, Jörg Gerhard; Veith, Roman; Veenendaal, Andreas
- PLoS ONE, Vol. 5, Issue 7
Single-molecule imaging of transcription factor binding to DNA in live mammalian cells
journal, March 2013
- Gebhardt, J. Christof M.; Suter, David M.; Roy, Rahul
- Nature Methods, Vol. 10, Issue 5
Live-cell 3D super-resolution imaging in thick biological samples
journal, October 2011
- Cella Zanacchi, Francesca; Lavagnino, Zeno; Perrone Donnorso, Michela
- Nature Methods, Vol. 8, Issue 12
Single-Molecule Imaging in Living Drosophila Embryos with Reflected Light-Sheet Microscopy
journal, February 2016
- Greiss, Ferdinand; Deligiannaki, Myrto; Jung, Christophe
- Biophysical Journal, Vol. 110, Issue 4
Extend the field of view of selective plan illumination microscopy by tiling the excitation light sheet
journal, January 2015
- Gao, Liang
- Optics Express, Vol. 23, Issue 5
Light-sheet microscopy using an Airy beam
journal, April 2014
- Vettenburg, Tom; Dalgarno, Heather I. C.; Nylk, Jonathan
- Nature Methods, Vol. 11, Issue 5
Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
journal, January 2012
- Olarte, Omar E.; Licea-Rodriguez, Jacob; Palero, Jonathan A.
- Biomedical Optics Express, Vol. 3, Issue 7
Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media
journal, January 2012
- Fahrbach, Florian O.; Rohrbach, Alexander
- Nature Communications, Vol. 3, Issue 1
Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain
journal, January 2012
- Silvestri, L.; Bria, A.; Sacconi, L.
- Optics Express, Vol. 20, Issue 18
Scanned light sheet microscopy with confocal slit detection
journal, January 2012
- Baumgart, Eugen; Kubitscheck, Ulrich
- Optics Express, Vol. 20, Issue 19
Confocal line scanning of a Bessel beam for fast 3D Imaging
journal, January 2014
- Zhang, P.; Phipps, M. E.; Goodwin, P. M.
- Optics Letters, Vol. 39, Issue 12
Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish
journal, January 2015
- Yang, Zhe; Mei, Li; Xia, Fei
- Biomedical Optics Express, Vol. 6, Issue 5
Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens
journal, December 2012
- Gao, Liang; Shao, Lin; Higgins, Christopher D.
- Cell, Vol. 151, Issue 6
Programmable Illumination and High-Speed, Multi-Wavelength, Confocal Microscopy Using a Digital Micromirror
journal, August 2012
- Martial, Franck P.; Hartell, Nicholas A.
- PLoS ONE, Vol. 7, Issue 8
Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy
journal, May 2012
- York, Andrew G.; Parekh, Sapun H.; Nogare, Damian Dalle
- Nature Methods, Vol. 9, Issue 7
Fast, 3D imaging via confocal line scanning of a Bessel beam using a single galvo mirror
conference, March 2014
- Zhang, Pengfei; Goodwin, Peter M.; Werner, James H.
- SPIE BiOS, SPIE Proceedings
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
journal, September 2006
- Betzig, E.; Patterson, G. H.; Sougrat, R.
- Science, Vol. 313, Issue 5793
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
journal, August 2006
- Rust, Michael J.; Bates, Mark; Zhuang, Xiaowei
- Nature Methods, Vol. 3, Issue 10
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
journal, December 2006
- Hess, Samuel T.; Girirajan, Thanu P. K.; Mason, Michael D.
- Biophysical Journal, Vol. 91, Issue 11
Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy
journal, January 2014
- Han, Jason J.; Kunde, Yuliya A.; Hong-Geller, Elizabeth
- Journal of Biomedical Optics, Vol. 19, Issue 1
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
journal, October 2014
- Chen, Bi-Chang; Legant, Wesley R.; Wang, Kai
- Science, Vol. 346, Issue 6208
Works referencing / citing this record:
Technical implementations of light sheet microscopy
journal, January 2018
- Elisa, Zagato; Toon, Brans; De Smedt, Stefaan C.
- Microscopy Research and Technique, Vol. 81, Issue 9
Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy
journal, April 2020
- Fang, Chunyu; Chu, Tingting; Yu, Tingting
- Nature Communications
Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited]
journal, January 2018
- Gustavsson, Anna-Karin; Petrov, Petar N.; Moerner, W. E.
- Optics Express, Vol. 26, Issue 10