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Title: Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood

Significant variation in the inherent degree of acetylation naturally exists in the xylem cell walls of Populus trichocarpa. During pretreatment, endogenous acetate hydrolyzes to acetic acid that can subsequently catalyze the breakdown of poplar wood, increasing the efficiency of biomass pretreatment. Poplar genotypes varying in cell wall composition were pretreated in 0.3% H 2SO 4 in non-isothermal batch reactors. Acetic acid released from the wood was positively related to sugar release during pretreatment ( R ≥ 0.9), and inversely proportional to the lignin content of the poplar wood ( R = 0.6). There is significant variation in wood chemistry among P. trichocarpa genotypes. As a result, this study elucidated patterns of cell wall deconstruction and clearly links carbohydrate solubilization to acetate release. Tailoring biomass feedstocks for acetate release could enhance pretreatment efficiencies.
 [1] ;  [2] ;  [2] ;  [3]
  1. Univ. of British Columbia, Vancouver, BC (Canada)
  2. Univ. of Wisconsin, Madison, WI (United States); Wisconsin Energy Institute, Madison, WI (United States)
  3. Univ. of British Columbia, Vancouver, BC (Canada); Wisconsin Energy Institute, Madison, WI (United States)
Publication Date:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
BioMed Central
Research Org:
Univ. of British Columbia, Vancouver, BC (Canada)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; plant-cell wall; hardwood prehydrolysis; enzymatic-hydrolysis; Arabidopsis-thaliana; acid pretreatment; corn stover; aspen wood; birch wood; autohydrolysis; acetate; acetyl group; xylan; pretreatment; biorefinery; advanced biofuels; 09 BIOMASS FUELS
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1358356