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Title: Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood

Abstract

Significant variation in the inherent degree of acetylation naturally exists in the xylem cell walls of Populus trichocarpa. During pretreatment, endogenous acetate hydrolyzes to acetic acid that can subsequently catalyze the breakdown of poplar wood, increasing the efficiency of biomass pretreatment. Poplar genotypes varying in cell wall composition were pretreated in 0.3% H 2SO 4 in non-isothermal batch reactors. Acetic acid released from the wood was positively related to sugar release during pretreatment ( R ≥ 0.9), and inversely proportional to the lignin content of the poplar wood ( R = 0.6). There is significant variation in wood chemistry among P. trichocarpa genotypes. As a result, this study elucidated patterns of cell wall deconstruction and clearly links carbohydrate solubilization to acetate release. Tailoring biomass feedstocks for acetate release could enhance pretreatment efficiencies.

Authors:
 [1];  [2];  [2];  [3]
  1. Univ. of British Columbia, Vancouver, BC (Canada)
  2. Univ. of Wisconsin, Madison, WI (United States); Wisconsin Energy Institute, Madison, WI (United States)
  3. Univ. of British Columbia, Vancouver, BC (Canada); Wisconsin Energy Institute, Madison, WI (United States)
Publication Date:
Research Org.:
Univ. of British Columbia, Vancouver, BC (Canada)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1356075
Alternate Identifier(s):
OSTI ID: 1358356
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; plant-cell wall; hardwood prehydrolysis; enzymatic-hydrolysis; Arabidopsis-thaliana; acid pretreatment; corn stover; aspen wood; birch wood; autohydrolysis; acetate; acetyl group; xylan; pretreatment; biorefinery; advanced biofuels; 09 BIOMASS FUELS

Citation Formats

Johnson, Amanda M., Kim, Hoon, Ralph, John, and Mansfield, Shawn D. Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0734-z.
Johnson, Amanda M., Kim, Hoon, Ralph, John, & Mansfield, Shawn D. Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood. United States. doi:10.1186/s13068-017-0734-z.
Johnson, Amanda M., Kim, Hoon, Ralph, John, and Mansfield, Shawn D. Thu . "Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood". United States. doi:10.1186/s13068-017-0734-z. https://www.osti.gov/servlets/purl/1356075.
@article{osti_1356075,
title = {Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood},
author = {Johnson, Amanda M. and Kim, Hoon and Ralph, John and Mansfield, Shawn D.},
abstractNote = {Significant variation in the inherent degree of acetylation naturally exists in the xylem cell walls of Populus trichocarpa. During pretreatment, endogenous acetate hydrolyzes to acetic acid that can subsequently catalyze the breakdown of poplar wood, increasing the efficiency of biomass pretreatment. Poplar genotypes varying in cell wall composition were pretreated in 0.3% H2SO4 in non-isothermal batch reactors. Acetic acid released from the wood was positively related to sugar release during pretreatment (R ≥ 0.9), and inversely proportional to the lignin content of the poplar wood (R = 0.6). There is significant variation in wood chemistry among P. trichocarpa genotypes. As a result, this study elucidated patterns of cell wall deconstruction and clearly links carbohydrate solubilization to acetate release. Tailoring biomass feedstocks for acetate release could enhance pretreatment efficiencies.},
doi = {10.1186/s13068-017-0734-z},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

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Cited by: 1 work
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Figures / Tables:

Table 1 Table 1: Associations between  wood acetate content and  other cell wall components or traits among  P. trichocarpa genotypes

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    Works referencing / citing this record:

    Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood [Supplemental Data]
    dataset, February 2017

    • Johnson, Amanda; Kim, Hoon; Ralph, John
    • figshare-Supplementary information for journal article at DOI: 10.1186/s13068-017-0734-z, 1 DOCX file (55.83 kB)
    • DOI: 10.6084/m9.figshare.c.3700927

      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.